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pCMV-PEmaxRNaseHtrunc

Plasmid

#207858

Purpose

Mammalian expression of PEmaxDRNaseH prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PEmaxDRNaseH

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

M-MLVRTDRNAseHrt

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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myrAkt delta4-129

Plasmid

#10841

Depositor

Richard Roth

Article

Kohn et al J Biol Chem. 1996 Sep 6. 271(36):21920-6

Insert

Akt (AKT1 Human)

Tags

HA and myr

Expression

Mammalian

Mutation

Deletion of PH domain (aa4-129)

Promoter

SV40

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GO

Plasmid

#204340

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-s/Gbetagamma ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET29b-dspB(E184Q)-6xHis

Plasmid

#175802

Purpose

Plasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

Insert

Dispersin B

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1D-eIF3bgi

Plasmid

#240040

Purpose

Human eIF3b, eIF3g and eIF3i subunits for further cloning or expression in insect cells for purification

Depositor

Venki Ramakrishnan

Article

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3b, g, i

Tags

eIF3i: HIS10

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-mGSDMD

Plasmid

#111560

Purpose

Express mouse GSDMD in E.coli

Depositor

Hongbo Luo

Article

Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

Insert

Gasdermin D (Gsdmd Mouse)

Tags

His, SUMO

Expression

Bacterial

Promoter

T7

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-LaminA-C-18

Plasmid

#55068

Purpose

Localization: Nuclear Envelope, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Lamin A (LMNA Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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iCAP-ROOT

Plasmid

#192262

Purpose

AAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsid

Depositor

Li Ye

Article

Wang et al Nature. 2022 Sep;609(7927):569-574. doi:

Insert

Synthetic construct for ROOT VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-NME3

Plasmid

#23574

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NME3 (NME3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDV06.2c_DART-VADAR_iRFP720-sensor

Plasmid

#200410

Purpose

pDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

Closed-loop DART VADAR sensor detecting iRFP720

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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