We narrowed to 1,249 results for: vent

1. pCX-SpiCee-Kras

   Plasmid

   #140838

   Purpose

   mammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-Kras
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. E407V Nuclear Myosin I YFP

   Plasmid

   #60242

   Purpose

   Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

   Depositor

   Primal de Lanerolle

   Article

   Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

   Insert

   Nuclear Myosin 1 (Myo1c Mouse)
   

   Use

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   E407V

   Promoter

   Available since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pAT-WT-HA-GFP11

   Plasmid

   #177719

   Purpose

   ER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFP

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   alpha-1-antitrypsin (SERPINA1 Human)
   

   Use

   Tags

   GFP11 and HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMALcPP-MS2cp

   Plasmid

   #203351

   Purpose

   Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria

   Depositor

   Thomas Cech

   Article

   Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;12

   Insert

   MS2 coat protein
   

   Use

   Tags

   Hx6-MBP

   Expression

   Bacterial

   Mutation

   V75E and A81G, to prevent capsid assembly

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. EC71173

   Plasmid

   #154061

   Purpose

   Level 1, Position 2 Golden Gate vector. HspHv17::Cre-U5-Cre

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Insert

   Cre recombinase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Introduction of U5 intron sequences, to prevent p…

   Promoter

   HvHSP17

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-SETin-FLAG-HA

   Plasmid

   #24999

   Depositor

   Judy Lieberman

   Article

   Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

   Insert

   SET (SET Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   silent mutations introduced to prevent siRNA targ…

   Promoter

   Available since

   Aug. 9, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pEn-C1.1

   Plasmid

   #61479

   Purpose

   Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

   Depositor

   Holger Puchta

   Article

   Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

   Insert

   AtU6-26:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   U6-26

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. A3Bi-Cas9n-UGI

   Plasmid

   #119135

   Purpose

   Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3B L1 intron-Cas9 nickase-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   L1 intron insertion

   Promoter

   CMV

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCX-Lyn-SponGee

   Plasmid

   #134776

   Purpose

   mammalian expression of Lyn-SponGee, a lipid raft-targeted variant of the cGMP buffer SponGee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

   Insert

   Lyn-SponGee
   

   Use

   Tags

   FLAG and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB1A3 EcoRI Methylase-AmilCP

    Plasmid

    #85166

    Purpose

    For methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.

    Depositor

    Robin Dowell

    Article

    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

    Insert

    EcoRI Methylase-AmilCP
    

    Use

    Tags

    AmilCP

    Expression

    Bacterial

    Mutation

    Promoter

    J23100 promoter

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only