We narrowed to 8,590 results for: dykddddk
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Plasmid#171216PurposeExpresses a mu-opioid receptor-based circular permuted green fluorescent protein sensorDepositorInsertM-SPOTIT1.1
UseLentiviralTagsFLAG tagExpressionMammalianPromoterCMVAvailable SinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG FLEX BARK p2A mCherry
Plasmid#117693PurposeCAG-FLEx-iBARK-p2A-mCherry: Viral expression vector for Cre-dependent Gaq silencingDepositorInsertBARKrgs peptide
UseCre/LoxTagsHA / FLAGAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 FLCN (WT) FLAG GFP
Plasmid#164978PurposeLentiviral vector for FLCN expression in mammalian cellsDepositorAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
(133) pcDNA3.1-Nup62-Flag-EPEA
Plasmid#160744PurposeExpresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymesDepositorInsertNup62-Flag-EPEA
TagsEPEA and FlagExpressionMammalianPromoterT7/CMVAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)::NL3F10H
Plasmid#141290PurposeNLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC3F10H
UseLuciferaseTagsFlag (x3) and His (x10)ExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-CD28TMD-NTEVp(H75E)
Plasmid#137829PurposeMESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV proteaseDepositorInsertFKBP-CD28TMD-NTEVp(H75E)
Tags3x FLAGExpressionMammalianMutationMutated Histidine 75 to Glutamic Acid in NTEVp fr…PromoterCMVAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT_FLAG-Mx PylRS
Plasmid#140011PurposePlasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianPromoterEF1Available SinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FoxJ1 promoter-IRES-EGFP-Flag-hChibby1
Plasmid#122964PurposeExpresses Flag-tagged Chibby1 in multiciliated cells under FoxJ1 promoter. It can be used as a cloning vector for gene of interest at SfiI sites.DepositorAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_WT POLI
Plasmid#131228PurposeExpression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cellsDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationCoding sequence has been codon optimised for expr…PromoterCMV enhancer + CMV promoterAvailable SinceDec. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-MCPIP1 (1-599)
Plasmid#127461PurposeExpressing Flag-tagged human MCPIP1 full lengh protein in mammalian cellsDepositorAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-Puro-2A-NLS-FLAG-cGAS
Plasmid#127656PurposeOver-express NLS-FLAG-cGASDepositorAvailable SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBM019
Plasmid#128179PurposeConstruct for generating stable Cas9-expressing Toxoplasma gondiiDepositorInsertssgRNA#1
SpCas9
Chloramphenicol acetyltransferase
UseCRISPR; Toxoplasma gondii expressionTags3X FLAG and Nuclear Localization SignalPromoterTUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-MYO1D
Plasmid#125125PurposeExpresses human FLAG-MYO1DDepositorAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_CD WT TET1
Plasmid#124082PurposeExpression of wild-type catalytic domain only form of TET1DepositorAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAH237
Plasmid#121146PurposeExpression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeastDepositorInsertsgRNA cassette
humanized Streptococcus pyogenes Cas9
UseCRISPRTagsFlagExpressionYeastPromoterS.pombe rrk1 and nmt41Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
nos-Cas9.874Z1
Plasmid#112685PurposeExpress hSpCas9 under nanos promoterDepositorInserthSpCas9
Tags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoternanosAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ubiq-Cas9.874W
Plasmid#112686PurposeExpress hSpCas9 under Ubiquitin-63E promoterDepositorInserthSpCas9
Tags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoterUbiquitin-63E promoterAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-RapGAP
Plasmid#118324PurposeExpression of RapGAPDepositorInsertRapGAP
TagsFlagExpressionMammalianPromoterCMVAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet2 CD Mut
Plasmid#72220PurposeExpress flag-tagged mouse Tet2 catalytic domain - catalytic dead mutant.DepositorInsertTen-Eleven Translocation 2 (Tet2 Mouse)
TagsflagExpressionMammalianMutationThe plasmid expresses TET2 catalytic domain (cata…PromoterCMVAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
BPK1169: CAG-DmrC-NLS-3xFLAG-P65
Plasmid#104564PurposeMammalian expression vector for DmrC fused to p65 activation domainDepositorInsertDmrC-p65
Tags3x FLAG and NLSExpressionMammalianPromoterCAGAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only