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pLPC-NMYC-mTRF1deltaBLM

Plasmid

#64163

Purpose

Retroviral vector expressing mouse TRF1 lacking BLM-binding motifs with N-terminal Myc tag

Depositor

Titia de Lange

Article

Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

Insert

mTRF1 (Terf1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Deleted amino acids 313-316 and 339-342 (both BLM…

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CHRFAM7ADelta2bp-YFP

Plasmid

#62637

Purpose

mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with YFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Expression

Mammalian

Mutation

there is a 2-bp deletion in exon 6 of CHRFAM7A an…

Promoter

CMV

Available Since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8m-WPRE

Plasmid

#162375

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-sumo-NSP12 (SARS-CoV-2)

Plasmid

#159107

Purpose

The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

Depositor

Thomas Tuschl

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Insert

NSP12

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8s-WPRE

Plasmid

#162374

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC-hTRF1

Plasmid

#64164

Purpose

Retroviral vector expressing human TRF1 with N-terminal MYC tag

Depositor

Titia de Lange

Article

Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG PSAM4-5HT3 HC IRES EGFP

Plasmid

#119740

Purpose

Chemogenetic activator expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Insert

PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Synthetic, Human)

Expression

Mammalian

Mutation

L131G, Q139L, Y217F, R420Q, R424D, R428A

Promoter

CAG

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDONRp5-p2 SspB-mCherry-CShroom3

Plasmid

#170977

Purpose

C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.

Depositor

Miki Ebisuya

Article

Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

Insert

CShroom3 (Shroom3 Mouse)

Use

Gateway cloning

Tags

SspB and mCherry

Mutation

Isoform X2, deleted aminoacids 1 - 1388

Available Since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8f-WPRE

Plasmid

#162376

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a 6xHis PreScission SARS-CoV-2 nsp13

Plasmid

#159390

Purpose

Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

Depositor

Tarun Kapoor

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Insert

N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression

Tags

His6 tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-MAPK14

Plasmid

#200973

Purpose

a mammalian expression vector to express MAPK14

Depositor

Jason Sheltzer

Article

Bhattacharjee et al Cell Chem Biol. 2023 Oct 19;30(10):1211-

Insert

mitogen-activated protein kinase 14 (MAPK14) (MAPK14 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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RUSH reporter-HMGB1-SBP-GFP

Plasmid

#172357

Purpose

Monitoring of HMGB1 subcellular localization by fluorescence videomicroscopy

Depositor

Guido Kroemer

Article

Liu et al Sci Rep. 2017 Nov 2;7(1):14915. doi: 10.

Insert

high mobility group box 1 (HMGB1 Human)

Use

Lentiviral

Tags

SBP-GFP

Promoter

CMV

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-N2-6MYC-hMID2

Plasmid

#51035

Purpose

mammalian expression of myc-tagged human MID2

Depositor

Timothy Cox

Article

Short et al BMC Cell Biol. 2002;3:1. Epub 2002 Jan 4

Insert

MID2 (MID2 Human)

Tags

MYC

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ASAP3-WPRE

Plasmid

#132331

Purpose

expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3

Use

AAV

Expression

Mammalian

Promoter

hSynapsin

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-DDX11-Flag-WT

Plasmid

#120727

Purpose

Expression of a Flag-tagged version of DDX11 WT in mammalian cells

Depositor

Francesca Pisani

Article

Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

Insert

DDX11 (DDX11 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pX330_CDC45

Plasmid

#244243

Purpose

Expresses SpCas9 and a sgRNA targeting the human CDC45 loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Minami et al Sci Adv. 2025 Mar 28;11(13):eadu8400. do

Insert

sgRNA targeting human CDC45 (CDC45 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-monSTIM1

Plasmid

#161795

Purpose

Mammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)

Depositor

Won Do Heo

Article

Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

Insert

MonSTIM1 (STIM1 Mustard Weed, Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-mOrange/CALR del52

Plasmid

#214768

Purpose

Mammalian expression of human CALR del52

Depositor

Norio Komatsu

Article

De Marchi et al Leuk Res. 2022 Aug;119:106883. doi: 10.1

Insert

human CALR del52 (CALR Human)

Use

Retroviral

Promoter

MSCV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV GFAP jGCaMP7b

Plasmid

#171118

Purpose

Astrocyte AAV-mediated expression of bright ultrasensitive protein calcium sensor under the GFAP promoter

Depositor

Thomas Oertner

Article

Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

Insert

jGCaMP7b

Use

AAV

Tags

6 His Tag, T7 Tag, and Xpress Tag

Expression

Mammalian

Promoter

GFAP

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET-15b_LC3B

Plasmid

#73949

Purpose

For recombinant expression of human LC3B/MAP1LC3B in E. coli

Depositor

Dieter Willbold

Article

Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

Insert

LC3B (MAP1LC3B Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only