We narrowed to 20,500 results for: ACE;

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pCsB

Plasmid

#136068

Purpose

Acceptor plasmid for even level position 2, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsC

Plasmid

#136069

Purpose

Acceptor plasmid for even level position 3, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsE

Plasmid

#136070

Purpose

Acceptor plasmid for even level position 4, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-moxGFP-Blast

Plasmid

#207710

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-mNeon-Zeo

Plasmid

#207731

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-C-miRFP670nano3-Puro

Plasmid

#207724

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-mNeon

Plasmid

#207698

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mNeon cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-V5

Plasmid

#207697

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-V5 cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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RIB VP64 R1

Plasmid

#161493

Purpose

CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2-VP64
gRNA (Targeting R1 in the RIB1 promoter)

Expression

Yeast

Promoter

pGAP, pPOR1, and pTEF2

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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3TERM_35S

Plasmid

#136114

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_35S (CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pEF1α-Cre-2A-miRFP670nano3

Plasmid

#207747

Purpose

Expression of the Cre recombinase linked to a miRFP670nano3 fluorescent marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-PGK-miRFP670nano3

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pFREE2-SpCas9-sgRNA

Plasmid

#179582

Purpose

Expresses spCas9 and gRNA that targets ColE1 plasmids for curing

Depositor

Kevin Solomon

Article

Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

Insert

Cas9

Expression

Bacterial

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pEPSA5Cas9syn

Plasmid

#206965

Purpose

S. aureus genome editing vector removed these two motifs AGGNNNNNGAT and CCAYNNNNNNTGT

Depositor

Daijie Chen

Article

Yang et al ACS Synth Biol. 2023 Oct 13. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV4-ZIKV-NS4A

Plasmid

#178845

Purpose

Mammalian expression of ZIKV ORF

Depositor

Judy Lieberman

Article

Sen Santara et al Proc Natl Acad Sci U S A. 2021 Nov 23;11

Insert

ZIKV-NS4A

Expression

Mammalian

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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dCas9v2

Plasmid

#103140

Purpose

TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

dCas9-VPR

Expression

Yeast

Mutation

Removed scaffold gRNA from original plasmid

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV4-ZIKV-NS3

Plasmid

#178844

Purpose

Mammalian expression of ZIKV ORF

Depositor

Judy Lieberman

Article

Sen Santara et al Proc Natl Acad Sci U S A. 2021 Nov 23;11

Insert

ZIKV-NS3

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pICH51266

Plasmid

#50267

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (1.3 kb ), 35s (Cauliflower Mosaic Virus) + 5'UTR omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pTE103

Plasmid

#59398

Purpose

MethyloBrick expression vector, fumC promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PfumC

Available Since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pYSD300

Plasmid

#212930

Purpose

Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

AGA1 (AGA1 Budding Yeast)

Expression

Yeast

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBP-SJM901

Plasmid

#72966

Purpose

Level 0 - SJM901 promoter (5' CTAT/3' GTAC fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

SJM901 promoter

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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qTAG-C-3xHA-Zeo

Plasmid

#207740

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-3xHA

Plasmid

#207707

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xHA cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pQadd2

Plasmid

#135662

Purpose

Clostridial vector, carrying lox511/66 and loxFAS/71 sites separated by restriction sites in order to add cargo. Use in combination with pQcre1 to insert cargo into lox sites, inserted with pQadd1F/R.

Depositor

Andrew Tolonen

Article

Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007

Insert

Lox511/66 and LoxFas/71

Use

Clostridial vector, pamβ1 origin

Expression

Bacterial

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pTE104

Plasmid

#59399

Purpose

MethyloBrick expression vector, coxB promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PcoxB

Available Since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pDEINO5

Plasmid

#179489

Purpose

Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

Depositor

Bogumil Karas

Article

Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

Insert

aacC1

Use

Synthetic Biology; Bacterial and yeast cloning

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-Pl-tetO1-deGFP-T500 (122p)

Plasmid

#70198

Purpose

Promoter panel

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deGFP

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NLS-GI-VP64-IRES-NLS-GAL4DBD-M13-SmBiT-FKF1

Plasmid

#183400

Purpose

Dual expression of GI-VP64 and GAL4DBD-M13-SmBiT-FKF1 in the nucleus

Depositor

Alice Ting

Article

Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

Insert

GI-VP64-IRES-GAL4DBD-M13-SmBiT-FKF1

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CDS_mALS

Plasmid

#136081

Purpose

Confers plant resistance to chlorsulfuron

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_mALS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-N-Puro-moxGFP

Plasmid

#207688

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-moxGFP cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
G1052C_BattB(GA)_EGFP_IRES-Spycatcher-sfmCherry3C(1-10)-P2A-shBleR

Plasmid

#200636

Purpose

Strong EGFP fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

EGFP

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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