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We narrowed to 30,644 results for: PLE

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Showing: 3391 - 3420 of 30644 results

  12. MARS-nGFP

    Plasmid
    #205234
    Purpose
    Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells
    Depositor
    Jeremy Baskin
    Article
    Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816
    Insert
    PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
    Tags
    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available Since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205234-plasmid-map-sequence-id-404747

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  15. lenti MS2-P65-HSF1_Hygro

    Plasmid
    #61426
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Insert
    MS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1A
    Available Since
    Dec. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    61426-plasmid-map-sequence-id-140565

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  16. pCDH-2xMARS-nGFP-puro

    Plasmid
    #205238
    Purpose
    Lentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody
    Depositor
    Jeremy Baskin
    Article
    Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816
    Insert
    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    Use
    Lentiviral
    Tags
    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available Since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205238-plasmid-map-sequence-id-403865

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  18. pAAV-hSyn-DIO {hCAR}off-{hM4Di-mCherry}on-W3SL

    Plasmid
    #111397
    Purpose
    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON hM4Di-mCherry (Gi-coupled DREADD for neuronal silencing), W3SL cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    hCAR (CXADR Human)
    hM4Di-mCherry
    Use
    AAV
    Tags
    Myc and mCherry
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available Since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    111397-plasmid-map-sequence-id-212158

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  19. G protein alpha-s(alpha3beta5/G226A)

    Plasmid
    #55798
    Purpose
    In addition to the mutations in alpha-s(alpha3beta5), this dominant negative G protein alpha-s mutant contains the G226A mutation, which impairs activating conformation changes in Switch II.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    alpha-s (alpha3beta5/G226A) (Gnas Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T, G226A in alpha…
    Promoter
    CMV
    Available Since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    55798-plasmid-map-sequence-id-112177

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  20. pQCXIP GFP-MOSPD2 W201E

    Plasmid
    #186471
    Purpose
    Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to EGFP in mammalian cells
    Depositor
    Catherine Tomasetto
    Article
    Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213
    Insert
    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    W201E (no more binding to lipid droplets)
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    186471-plasmid-map-sequence-id-366065

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  23. G protein alpha-s(alpha3beta5/G226A/A366S)

    Plasmid
    #55797
    Purpose
    This highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    alpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T, G226A, A366S i…
    Promoter
    CMV
    Available Since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    55797-plasmid-map-sequence-id-112181

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Showing: 3391 - 3420 of 30644 results