We narrowed to 5,173 results for: pcDNA3.1+

1. pAL204

   Plasmid

   #22282

   Purpose

   Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Vh-HA-3xFRB

   Plasmid

   #20223

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   3xFRB (MTOR Human)
   

   Use

   Tags

   HA and Vh

   Expression

   Mammalian

   Mutation

   One copy of FRB has a T2098L mutation

   Promoter

   Available since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3-HA-ERK2 D319N

   Plasmid

   #8979

   Depositor

   John Blenis

   Article

   Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

   Insert

   ERK2 (Mapk1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D319N

   Promoter

   Available since

   Oct. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

4. mc2 blast

   Plasmid

   #206217

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSL2621 (pCas8_Vch)

   Plasmid

   #200893

   Purpose

   Expresses human codon-optimized V. cholerae Cas8 from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCas8
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSL2623 (pCas6_Vch)

   Plasmid

   #200895

   Purpose

   Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCas6
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FLAG SMG1 T3550A/S3556A

   Plasmid

   #199574

   Purpose

   Expresses FLAG-tagged mutated SMG1

   Depositor

   Michael Kastan

   Article

   McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

   Insert

   SMG1 (SMG1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T3550A and S3556A

   Promoter

   CMV

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mH2A1.2-CT-MYC

   Plasmid

   #45168

   Depositor

   Brian Chadwick, Hunt Willard

   Article

   Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

   Insert

   H2Afy (MACROH2A1 Human)
   

   Use

   Tags

   HIS and MYC

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

9. DAXX-myc-his

   Plasmid

   #1852

   Depositor

   Philip Leder

   Article

   Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

   Insert

   DAXX (Daxx Mouse)
   

   Use

   Tags

   his and myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pDN42

    Plasmid

    #61345

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS10 and PKIt NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSL2622 (pCas7_Vch)

    Plasmid

    #200894

    Purpose

    Expresses human codon-optimized V. cholerae Cas7 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCas7
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Integrin-αv Headpiece M400GC

    Plasmid

    #159637

    Purpose

    Contains integrin alpha v residues 1–594 with the M400C mutation and Gly inserted prior to residue 400

    Depositor

    Timothy Springer

    Article

    Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

    Insert

    alpha v headpiece M400GC
    

    Use

    Tags

    6x His and Strep II

    Expression

    Mammalian

    Mutation

    M400C

    Promoter

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-H2B-Venus

    Plasmid

    #87614

    Purpose

    Venus (YFP) fused to histone H2B.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    H2B-Venus (H2BC8 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mc2 mCherry EF1a

    Plasmid

    #206225

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA-3xFLAG-TCAB1

    Plasmid

    #51630

    Purpose

    Mammalian expression of 3xFLAG tagged TCAB1, N-terminal.

    Depositor

    Steven Artandi

    Article

    Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

    Insert

    TCAB1 (WRAP53 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

16. VinTS-tCRMod-GGSGGS5

    Plasmid

    #111763

    Purpose

    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 module

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV2-hNCOA6:V5

    Plasmid

    #63585

    Purpose

    A subunit of the Trithorax-related (Trr) methyltransferase complex.

    Depositor

    Kenneth Irvine

    Article

    Oh et al Cell Rep. 2014 Jul 24;8(2):449-59. doi:

    Insert

    NCOA6 (NCOA6 Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pspCas9-3'UTR-Tetra-com-vector

    Plasmid

    #132553

    Purpose

    Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Inserts

    Cas9
    sgRNA scaffold with com replacing the Tetraloop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAL188

    Plasmid

    #22277

    Purpose

    Expresses Tiam DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    TiamDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EYFP-Clathrin

    Plasmid

    #20921

    Depositor

    Xiaowei Zhuang

    Article

    Rust et al Nat Struct Mol Biol. 2004 Jun . 11(6):56

    Insert

    Clathrin, light polypeptide (Lca) (Clta Mouse)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA flag-Pcbp2

    Plasmid

    #184028

    Purpose

    Codon optimized Pcbp2 clone with N-terminal flag tag

    Depositor

    Peter Stoilov

    Article

    Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

    Insert

    Pcbp2 (Pcbp2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Codon optimized

    Promoter

    CMV

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFH2.21_mCherry-MS2x6

    Plasmid

    #205536

    Purpose

    Express mCherry-MS2x6 (export tag consisting of 6 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-FLAG-Fos DD

    Plasmid

    #8968

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S362D, S374D

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

24. hGFAP R239C

    Plasmid

    #169476

    Purpose

    Single p.Arg239Cys(R239C) mutations was individually inserted by site-directed mutagenesis into the wild-type hGFAP

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al Experimental Results (2020), Volume 1, e

    Insert

    EGFP-hGFAP (GFAP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed R 239 to C

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. HA-EcPPK1

    Plasmid

    #159279

    Purpose

    Expresses a HA-tagged E. coli PPK1 in mammalian cell lines

    Depositor

    Michael Downey

    Article

    Bondy-Chorney et al Cell Rep. 2020 Oct 27;33(4):108318. doi:

    Insert

    HA-tagged Polyphosphate Kinase 1
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CMV-2xFLAG-Atoh1

    Plasmid

    #101734

    Purpose

    Expresses mouse Atoh1 with FLAG tag

    Depositor

    Albert Edge

    Article

    Parker et al Hum Gene Ther Methods. 2014 Feb;25(1):1-

    Insert

    Atoh1 (Atoh1 Mouse)
    

    Use

    Tags

    2x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTS2396-Tier1-PhCMV-4xCOX8_Ypet

    Plasmid

    #169534

    Purpose

    Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven miochondria-localized yellow fluorescent protein Ypet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pNIR-FbGFPY58/C-JIP

    Plasmid

    #184684

    Purpose

    NIR-Fb-based JNK inhibitor

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-FbGFPY58/C-JIP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EGFP-PH

    Plasmid

    #96948

    Purpose

    EGFP-PLC pleckstrin homology domain for CALI validation

    Depositor

    Marcel Bruchez

    Article

    He et al Nat Methods. 2016 Mar;13(3):263-268. doi

    Insert

    Pleckstrin homology domain
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. perbB2K732M-ECFP

    Plasmid

    #40269

    Depositor

    Martin Offterdinger

    Article

    Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

    Insert

    erbB2 (ERBB2 Human)
    

    Use

    Tags

    ecfp

    Expression

    Mammalian

    Mutation

    K732M

    Promoter

    CMV

    Available since

    Jan. 4, 2013

    Availability

    Academic Institutions and Nonprofits only