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Showing: 5651 - 5700 of 17798 results

  12. pUCM-CLYBL-NGN2-BRN3A

    Plasmid
    #165597
    Purpose
    Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.
    Depositor
    Carsten Bönnemann
    Article
    Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    BRN3A (POU4F1 Human)
    EGFP
    Puromycin N-acetyl transferase
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Expression
    Mammalian
    Promoter
    CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…
    Available Since
    March 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165597-plasmid-map-sequence-id-326012

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  13. pαH-S-GSAS/XBB.1.5

    Plasmid
    #212991
    Purpose
    "Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.5 variant
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    pαH-S-GSAS/XBB.1.5 (S )
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    (T19I,Del24/26,A27S,V83A,G142D,Del144,H146Q,Q183E…
    Promoter
    CMV
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212991-plasmid-map-sequence-id-422311

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  17. pSLIK-NFLAG-hEWSR1G511A-CMyc

    Plasmid
    #50734
    Purpose
    Produces lentivirus expressing human EWSR1G511A mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G
    Depositor
    Zissimos Mourelatos
    Article
    Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91
    Insert
    EWSR1 G511A (EWSR1 Human)
    Use
    Lentiviral
    Tags
    FLAG and Myc
    Mutation
    Changed Gly 511 to Ala
    Promoter
    TRE
    Available Since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50734-plasmid-map-sequence-id-481096

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  20. BAP1-C91A (siRNA resistant)

    Plasmid
    #108438
    Purpose
    Stable expression of BAP1-C91A (catalytically inactive mutant) in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.
    Depositor
    Emanuela Felley-Bosco, Rolf Stahel
    Article
    Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-
    Insert
    BAP1 (BAP1 Human)
    Expression
    Mammalian
    Mutation
    C91A: c.271T>G, c.272G>C; others: c.1641C&g…
    Promoter
    CMV
    Available Since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    108438-plasmid-map-sequence-id-209210

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  23. pαH-S-RRAR-OMICRON.BA.5

    Plasmid
    #213071
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 with with furin side intact
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    pαH-S-RRAR-OMICRON.BA.5 (S )
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685, furin site inta…
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    213071-plasmid-map-sequence-id-422361

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  25. pSLIK-NFLAG-hEWSR1P552L-CMyc

    Plasmid
    #50736
    Purpose
    Produces lentivirus expressing human EWSR1P552L mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G
    Depositor
    Zissimos Mourelatos
    Article
    Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91
    Insert
    EWSR1 P552L (EWSR1 Human)
    Use
    Lentiviral
    Tags
    FLAG and Myc
    Mutation
    Changed Pro 552 to Leu
    Promoter
    TRE
    Available Since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  31. pBOBI C-MYC GAC F7

    Plasmid
    #227828
    Purpose
    To amplify the virus that expresses C-MYC GAC F7 with 33 residues mutated to alanine at the interface for interacting with PDZD8
    Depositor
    Sheng-cai Lin
    Article
    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414
    Insert
    GAC isoform of F7 (GLS Human)
    Use
    Lentiviral
    Tags
    MYC
    Expression
    Mammalian
    Mutation
    P137, L139, E140, L142, Y145, G150, Q151, E152, K…
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    227828-plasmid-map-sequence-id-488851

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  32. pGEX GAC F7

    Plasmid
    #227829
    Purpose
    To express in E. coli GAC F7 with 33 residues mutated to alanine at the interface for interacting with PDZD8
    Depositor
    Sheng-cai Lin
    Article
    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414
    Insert
    GAC isoform of F7 (GLS Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    P137, L139, E140, L142, Y145, G150, Q151, E152, K…
    Available Since
    July 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    227829-plasmid-map-sequence-id-479453

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  33. pHM1 KGA-33A

    Plasmid
    #227836
    Purpose
    Used for microinjection of nematodes for expression of KGA-33A, GLS with 33 residues mutated to alanine at the interface for interacting with PDZD8
    Depositor
    Sheng-cai Lin
    Article
    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414
    Insert
    KGA isoform (GLS Human)
    Tags
    GFP
    Expression
    Worm
    Mutation
    P137, L139, E140, L142, Y145, G150, Q151, E152, K…
    Available Since
    July 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    227836-plasmid-map-sequence-id-479443

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  34. pGEX KGA F7

    Plasmid
    #227822
    Purpose
    To express in E. coli KGA F7 with 33 residues mutated to alanine at the interface for interacting with PDZD8
    Depositor
    Sheng-cai Lin
    Article
    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414
    Insert
    KGA isoform of F7 (GLS Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    P137, L139, E140, L142, Y145, G150, Q151, E152, K…
    Available Since
    June 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    227822-plasmid-map-sequence-id-477347

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  35. pBOBI C-MYC KGA F7

    Plasmid
    #227821
    Purpose
    To amplify the virus that expresses C-MYC KGA F7 with 33 residues mutated to alanine at the interface for interacting with PDZD8
    Depositor
    Sheng-cai Lin
    Article
    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414
    Insert
    KGA isoform of F7 (GLS Human)
    Use
    Lentiviral
    Tags
    MYC
    Expression
    Mammalian
    Mutation
    P137, L139, E140, L142, Y145, G150, Q151, E152, K…
    Available Since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    227821-plasmid-map-sequence-id-477304

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  36. pαH-S-GSAS/EG.5.1

    Plasmid
    #218668
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.EG.5.1 variant
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    pαH-S-GSAS/EG.5.1 (S )
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    CMV
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218668-plasmid-map-sequence-id-433275

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  38. pαH-S-GSAS/EG.5

    Plasmid
    #212990
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.EG5 variant
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    pαH-S-GSAS/EG.5 (S )
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    CMV
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212990-plasmid-map-sequence-id-423192

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  50. pHis-TEV-Nsp15

    Plasmid
    #187656
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004
    Insert
    Nsp15 (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7lac
    Available Since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    187656-plasmid-map-sequence-id-388768

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Showing: 5651 - 5700 of 17798 results