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pCAG_Xph20-mEos3.2-CCR5TC

Plasmid

#135532

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to mEos3.2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Use

Tags

mEos3.2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

Plasmid

#183410

Purpose

piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

KRAB-dCas9-ecDHFR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-YAPS127A

Plasmid

#166458

Purpose

Expresses fusion of mEGFP and YAPS127A

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A (YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-p300

Plasmid

#191764

Purpose

Expression of EGFP fused to core p300 histone acetyltransferase

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

FRB-EGFP-p300 core (EP300 Human)

Use

Tags

EGFP (N-terminal of p300 core) and FRB (N-termina…

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1alpha

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-eGFP-CCR5TC

Plasmid

#135530

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-YAP

Plasmid

#166457

Purpose

Expresses fusion of mEGFP and YAP

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAP (YAP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-ISG15 C78SN13Y

Plasmid

#165106

Purpose

bacterial expressio of a GST fusion of Human ISG15 C78SN13Y

Depositor

Arthur Haas

Article

Narasimhan et al J Biol Chem. 2005 Jul 22. 280(29):27356-

Insert

ISG15 (ISG15 Human)

Use

Tags

GST from plasmid.

Expression

Bacterial

Mutation

C78SN13Y; The sequence of ISG15-C78S/N13Y contai…

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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CD154-His-bio

Plasmid

#52337

Purpose

Expresses full-length Tumor necrosis factor ligand superfamily member 5 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

CD154 (CD40LG Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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p80-Katanin-mNeonGreen

Plasmid

#191762

Purpose

Expression of mNeonGreen fused to p80-Katanin

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

p80-Katanin-mNeonGreen (KATNB1 Human)

Use

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-FLEX-EGFP-WPRE

Plasmid

#51504

Purpose

Can be used to generate AAV virus that will express EGFP in the presence of Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

phSyn1

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-mNeonGreen-CCR5TC

Plasmid

#135534

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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PLKO.1-YAP shRNA2

Plasmid

#166485

Purpose

Human YAP RNAi

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

shRNA for human YAP (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSicoR p53

Plasmid

#12090

Depositor

Tyler Jacks

Article

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Insert

p53 shRNA (Trp53 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

EGFP is expressed from this plasmid as a marker, …

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph15-mNeonGreen-CCR5TC

Plasmid

#135533

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-FLEX-tdTomato-WPRE

Plasmid

#51505

Purpose

Can be used to generate AAV virus that will express tdTomato in the presence of Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdTomato

Use

AAV

Tags

Expression

Mutation

Promoter

phSyn1

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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pNK2657

Plasmid

#219741

Purpose

MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

hispidin- synthase from Mycena citricolor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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Pet22b-YAP

Plasmid

#166443

Purpose

Bacterial expression of 6x His tagged YAP

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP (YAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-neo-YAP

Plasmid

#166468

Purpose

Plasmid coding for YAP and neomycin resistance in a lentiviral backbone.

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

YAP, Neo (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 ORF3B

Plasmid

#141272

Purpose

Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF3B

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pPGK-T7/2-CD44v2-10 (fl)

Plasmid

#137824

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v2-10 (fl) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

full length and mutations A282T, T483A, N535D, S5…

Promoter

PGK

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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