We narrowed to 5,473 results for: KRAS

1. SpCas9 TadCBEa

   Plasmid

   #193782

   Purpose

   Express TadCBEa (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDa-SpCas9D10A
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTol2CG2-QUAS5x:GFPNLS-SV40pA

   Plasmid

   #155122

   Purpose

   Tol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QUAS5x:GFPNLS
   

   Use

   Zebrafish expression

   Promoter

   QUAS5x-E1b

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJC5-Flag-SAMTOR

   Plasmid

   #102420

   Purpose

   3rd generation lentiviral vector expressing FLAG SAMTOR in mammalian cells driven by UBC promoter

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   SAMTOR (BMT2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDA_936

   Plasmid

   #216094

   Purpose

   Cas12a [EnAs] knockout targeting CD46, CD47, CD55, CD81, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD46, CD47, CD55, CD81 guides
   

   Use

   CRISPR and Lentiviral; Positive controls

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA028

   Plasmid

   #216008

   Purpose

   Fragmid fragment: (Cas protein) nickase Cas for base editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   nCas9 (D10A)_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AA029

   Plasmid

   #216009

   Purpose

   Fragmid fragment: (Cas protein) nickase Cas for base editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   nCas9-NG (D10A)_v1.1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRDA_889

   Plasmid

   #216080

   Purpose

   ITRs from AAV2, destination vector

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   ITRs from AAV2
   

   Use

   AAV; Destination

   Expression

   Mammalian

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQCXIX_sh_HOIP/RNF31_#6

   Plasmid

   #75252

   Purpose

   retroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction control

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   shRNA against human RNF31 (RNF31 )
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. p2T-CAG-SpCas9-BlastR

   Plasmid

   #107190

   Purpose

   Confers constitutive expression of SpCas9

   Depositor

   Richard Sherwood

   Article

   Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

   Insert

   SpCas9 (NEWENTRY )
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Bacterial and Mammalian

   Promoter

   Chicken ß-actin

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PET-WT SpCas9-NLS-6xHis

    Plasmid

    #207373

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    WT SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. mEmerald-Caveolin-C-10

    Plasmid

    #54025

    Purpose

    Localization: Membrane, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Hanson et al Traffic. 2013 Jun;14(6):663-77. doi: 10.

    Insert

    Caveolin (CAV1 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pcDNA-D2s-L-Venus

    Plasmid

    #19966

    Depositor

    Jonathan Javitch

    Article

    Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

    Insert

    D2 short variant-monomeric Venus (DRD2 Aequorea Victoria, Human)
    

    Tags

    Flag and monomeric Venus

    Expression

    Mammalian

    Available Since

    Jan. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-cT3G-cERH-ZNF281

    Plasmid

    #222923

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of ZNF281

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    ZNF281 (ZNF281 Human)
    

    Expression

    Mammalian

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. LLP786_SpyCatcher-EZH2-FL-CO

    Plasmid

    #211779

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-KRAB
    

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpCas9 TadCBEe

    Plasmid

    #193836

    Purpose

    Express TadCBEe (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDe-SpCas9D10A-2xUGI
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti_CamKIIa_mKate2

    Plasmid

    #96942

    Purpose

    To label mature excitatory iPSC-derived human neurons

    Depositor

    Oleksandr Shcheglovitov, Ricardo Dolmetsch

    Article

    Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

    Insert

    far-red fluorescent protein
    

    Use

    Lentiviral

    Mutation

    E87D (please see depositor comments below)

    Promoter

    CaMKIIa

    Available Since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

    Plasmid

    #172501

    Purpose

    Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    DisCas7-11 full locus with Golden gate acceptor site for spacer
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pProEx M-Ras(G22C) CL

    Plasmid

    #224268

    Purpose

    Protein purification of M-Ras (G22C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    M-Ras (MRAS Human)
    

    Expression

    Bacterial

    Mutation

    G22C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pProEx Rit1(G47C) CL

    Plasmid

    #224267

    Purpose

    Protein purification of Rit1 (G47C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rit1 (RIT1 Human)
    

    Expression

    Bacterial

    Mutation

    G47C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pProEx Ypt1(S17C) CL

    Plasmid

    #224265

    Purpose

    Protein purification of Ypt1 (S17C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Ypt1 (YPT1 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    S17C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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