We narrowed to 36,206 results for: NAM

1. pcDNA3-TrxRFP2

   Plasmid

   #172322

   Purpose

   an improved fluorescent biosensor for thioredoxin redox

   Depositor

   Huiwang Ai

   Article

   Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

   Insert

   TrxRFP2, an improved version from TrxRFP1
   

   Use

   Tags

   His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKM446 (Flag-DAS+4))

   Plasmid

   #108321

   Purpose

   Orbit integrating plasmid for C-terminal tagging with degradation tag DAS+4, using hygromycin as a selection marker.

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Insert

   attB-Flag-DAS+4
   

   Use

   Orbit integrating plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-miRFP720

   Plasmid

   #197213

   Purpose

   Expresses the protein of miRFP720 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   miRFP720
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIS.MTRIA-MCH

   Plasmid

   #184606

   Purpose

   For expression of a fluorescent sensor for melanin-concentrating hormone in mammalian cells

   Depositor

   Hiroshi Hibino, Daisuke Ino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-MCH
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSF11-wmiRFP670-2-T2A-HO1

   Plasmid

   #197250

   Purpose

   Expresses the protein of wmiRFP670-2-T2A-HO1 in neurons of C. elegans

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   wmiRFP670-2-T2A-HO1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSET-His-APEX2

   Plasmid

   #201471

   Purpose

   Recombinant soluble APEX2 for SLAPSHOT labeling

   Depositor

   Yuh-Nung Jan

   Article

   Tuomivaara et al Commun Biol. 2024 Aug 29;7(1):1060. doi:

   Insert

   APEX2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV.Syn.MTRIA-OT

   Plasmid

   #184594

   Purpose

   For production of AAV for a fluorescent sensor for oxytocin

   Depositor

   Hiroshi Hibino, Daisuke Ino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-OT
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Human synapsin promoter

   Available since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-E2-Crimson

   Plasmid

   #197196

   Purpose

   Expresses the protein of E2-Crimson in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   E2-Crimson
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO-cell-mEm

   Plasmid

   #185595

   Purpose

   Lentiviral construct for expression of mEmerald

   Depositor

   Jarrod Johnson

   Article

   Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

   Insert

   cell-mEm
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Ateam1.03-nD/nA/pRSETB

    Plasmid

    #51960

    Purpose

    bacterial expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

    Insert

    ATeam1.03-nD/nA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTJV1ScKm-rpoB

    Plasmid

    #166980

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    NeoR/KanR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. VinculinTS-A50I

    Plasmid

    #111827

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind talin

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (A50I)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed alanine 50 to isoleucine

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSF11-wemiRFP670-T2A-HO1

    Plasmid

    #197243

    Purpose

    Expresses the protein of wemiRFP670-T2A-HO1 in neurons of C elegan

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wemiRFP670-T2A-HO1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTC150

    Plasmid

    #70014

    Purpose

    'Donor only' control plasmid for gene targeting of tomato ANT1 gene. Contains donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    Donor + GVR
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDRFLIP38

    Plasmid

    #65516

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-edCit-attR1 and attR2-edCer-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Skylan-S-(GGGGS)x3-β-Actin

    Plasmid

    #128938

    Purpose

    fluorescnece reporter of β-Actin with Skylan-S. Express in mammalian cells

    Depositor

    Shimon Weiss

    Article

    Yi et al Biomed Opt Express. 2019 Apr 16;10(5):24

    Insert

    Skylan-S
    

    Use

    Tags

    β-actin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pXY027

    Plasmid

    #98915

    Purpose

    Express FtsZ tagged with GFP in E coli

    Depositor

    Jie Xiao

    Article

    Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

    Insert

    FtsZ
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p006-Banana-Late

    Plasmid

    #112251

    Purpose

    ATF1 from Saccharomyces cerevisiae under osmY stationary phase promoter; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    ATF1 under osmY promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    osmY

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. VinculinTS-I997A

    Plasmid

    #111828

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind actin

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (I997A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed isoleucine 997 to alanine

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PHY23-phage-ubc-nls-ha-stdMCP-tdTomato

    Plasmid

    #164044

    Purpose

    Expression of stdMCP-tdTomato in mammalian cells

    Depositor

    Baohui Chen

    Article

    Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

    Insert

    stdMCP-tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C (UBC)

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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