We narrowed to 13,713 results for: cas9

1. CMVp-dCas9-3xNLS-VP64 (Construct 1)

   Plasmid

   #55195

   Purpose

   Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VP64

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/hUBC

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pspCas9-Klenow-ST2-com-vector

   Plasmid

   #176235

   Purpose

   Vector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. NLS-dCas9-HA-NLS-GFP-VP16

   Plasmid

   #183923

   Purpose

   VP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFP

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   dCas9-GFP-VP16
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCCL-PGK-SPdCas9-BFP-DNMT1

   Plasmid

   #66818

   Purpose

   dCas9 fused to BFP and the human DNMT1 catalytic domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dCas9-BFP-DNMT1, catalytic domain (DNMT1 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-FLEX-SaCas9-U6-sgKcnq3

   Plasmid

   #159911

   Purpose

   Mutagenesis of Kcnq3

   Depositor

   Larry Zweifel

   Insert

   Kcnq3 (Kcnq3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2T-CMV-evoCjCas9-TadCDd-BlastR

   Plasmid

   #202558

   Purpose

   Mammalian expression of evoCjCas9 TadCDd

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-TadCDd
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LLP457 pGK-dCas9-Suntag-BFP

   Plasmid

   #100957

   Purpose

   BPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tag

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, SunTag array (10xGCN4)
   

   Use

   CRISPR

   Tags

   3xHA and BFP

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

   Plasmid

   #164471

   Purpose

   Expresses sgRNA targeting human GJA1 locus

   Depositor

   Markus Kuehn

   Article

   Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

   Insert

   Gap junction alpha-1 (GJA1) (GJA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. iF58 PB-Zim3-dCas9-mScarlet-Puro

   Plasmid

   #204728

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-FLEX-SaCas9-U6-sgOprk1

    Plasmid

    #159903

    Purpose

    Mutagenesis of Oprk1

    Depositor

    Larry Zweifel

    Insert

    Oprk1 (Oprk1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CMV-FLEX-SaCas9-U6-sgTh(2)

    Plasmid

    #209198

    Purpose

    Mutagenesis of Th

    Depositor

    Larry Zweifel

    Article

    Yu et al Neuron. 2021 Apr 21;109(8):1365-1380.e5.

    Insert

    Th (Th Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E)

    Plasmid

    #120293

    Purpose

    AAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffold

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    N-terminal fragment of SpyCas9
    

    Use

    AAV and CRISPR

    Tags

    SV40-NLS and split-intein

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJEP304-pAAV-EFS-dSaCas9-VP64-pA

    Plasmid

    #113679

    Purpose

    A EFS driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    NLS and VP64

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti GFAABC1D-dCas9-KRAB-MeCP2

    Plasmid

    #194701

    Purpose

    Expresses dCas9-KRAB-MeCP2 in Astrocytes

    Depositor

    Timothy Jarome

    Article

    Farrell et al Learn Mem. 2023 Mar 15;30(3):70-73. doi:

    Insert

    GFAABC1D promoter
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    None

    Promoter

    GFAABC1D

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

    Plasmid

    #195741

    Purpose

    Base editing for making a pre-stop codon in mmoX using phenol inducible system

    Depositor

    Seung-Goo Lee

    Article

    Jeong et al Chem Eng J, 2023

    Insert

    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Po (phoenol-inducible promoter)

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSCV-LTR-dCas9-p65AD-BFP

    Plasmid

    #46913

    Purpose

    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    dCas9-p65AD-BFP fusion
    Puromycin resistance
    

    Use

    CRISPR and Retroviral

    Tags

    2xNLS, BFP, and VP64 domain

    Expression

    Mammalian

    Mutation

    Promoter

    LTR and PGK

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-UGI-nCas9(H840A)-GSVG

    Plasmid

    #187411

    Purpose

    DddA GSVG variant

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA GSVG variant
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. G1333 DddAtox-N–SaKKH-Cas9(D10A)

    Plasmid

    #157837

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    G1333 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV hUbC-dCas9-dMQ1-EGFG

    Plasmid

    #89794

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR and Lentiviral

    Tags

    3*FLAG and T2A-EGFP

    Expression

    Mammalian

    Mutation

    C141S, TGC-->TCC; S317A, AGC-->GCC

    Promoter

    hUbC

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-1BPNLS-Cas9-1BPNLS-2AGFP

    Plasmid

    #87109

    Purpose

    BPNLS-Cas9-BPNLS-2AGFP expression plasmid

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    1BPNLS-Cas9-1BPNLS-2A-EGFP
    

    Use

    CRISPR

    Tags

    3xFLAG (on Cas9)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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