We narrowed to 6,573 results for: tac

1. pKIR1.1

   Plasmid

   #85758

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Plant

   Mutation

   Promoter

   AtRPS5A

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEFIRES-P-mCherry-HPos

   Plasmid

   #87159

   Purpose

   Fluorescent marker of lipid droplets (LDs)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   HPos
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1-alpha

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT2-cryR;cldn5bP1Egfp

   Plasmid

   #90158

   Purpose

   contains cldn5b specific gene promoter driving expression of eGFP

   Depositor

   Nathan Lawson

   Article

   Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

   Insert

   cldn5bP1Egfp (cldn5b Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti_EF1a_Flag_EGFP

   Plasmid

   #183117

   Purpose

   Expresses flag-tagged EGFP in mammalian cells

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMVV102

   Plasmid

   #215308

   Purpose

   Expresses sfGFP under induction with the IPTG Marionette Cassette (LacIAM + PTac-sfGFP) with a BBR1 backbone.

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   PTac

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOD_A1111

   Plasmid

   #91032

   Purpose

   Module A, Promoter: OsAct1, Gene: TaCas9, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TaCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   OsAct1

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-Rab7Q67L

   Plasmid

   #169038

   Purpose

   Express human constitutively active form of Rab7a(Q67L) in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

   Insert

   Rab7a (RAB7A Human)
   

   Use

   Tags

   acGFP1

   Expression

   Mammalian

   Mutation

   Q67L

   Promoter

   CMV

   Available since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDNR-gRNA

   Plasmid

   #206990

   Purpose

   A plasmid for expression of SaCas9-sgRNA targeting donor plasmids

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Insert

   Donor plasmid-targeting SaCas9-gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-N1-RACK1

   Plasmid

   #41088

   Depositor

   Anna Huttenlocher

   Article

   Cox et al Mol Biol Cell. 2003 Feb;14(2):658-69.

   Insert

   Receptor for activated C kinase (RACK1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBRα-β flap (831-1057)

    Plasmid

    #53734

    Purpose

    Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    alpha NTD-Beta flap fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. mADAM17 in pcDNA3.1

    Plasmid

    #19141

    Depositor

    Zena Werb

    Article

    Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

    Insert

    ADAM17 (Adam17 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pAAV CMV GCGR-V5-sTurboID (N)

    Plasmid

    #153009

    Purpose

    Expresses split-TuboID N-terminal fragment fused to GCGR

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    GCGR-sTurboID (N) (L73)
    

    Use

    AAV

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3 CMV sTurboID (C)-Arrestin-GFP

    Plasmid

    #153010

    Purpose

    Expresses split-TurboID C-terminal fragment fused to arrestin

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID (C)(G74)-Arrestin
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBT9-NF1-GRD

    Plasmid

    #19993

    Depositor

    Fuyuhiko Tamanoi

    Article

    Morcos et al Mol Cell Biol. 1996 May . 16(5):2496-503

    Insert

    Neurofibromatosis type I (NF1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAJM.336

    Plasmid

    #108528

    Purpose

    LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    LacIAM + PTac-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LacI Q18M A47V F161Y

    Promoter

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. PC1-29-9-2_GAL4-VHL

    Plasmid

    #200434

    Purpose

    Expresses the GAL4 DNA Binding domain linked to the VHL E3 Ubiquitin Ligase

    Depositor

    Xue Gao

    Article

    Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

    Insert

    GAL4-VHL
    

    Use

    Tags

    GAL4, VHL

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Smac(1-60)mCherry

    Plasmid

    #40880

    Depositor

    Douglas Green

    Article

    Tait et al Dev Cell. 2010 May 18;18(5):802-13.

    Insert

    Smac(1-60)mCherry (DIABLO Human)
    

    Use

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA5-puro-eGFP-HEC1

    Plasmid

    #114022

    Purpose

    Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiGuide_mCherry_sgEPAS1#1

    Plasmid

    #183122

    Purpose

    pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgEPAS1-1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-PIK3C2B

    Plasmid

    #161989

    Purpose

    Mammalian expression vector containing PIK3C2B tagged with GFP

    Depositor

    Volker Haucke, David Bryant

    Article

    Marat et al Science. 2017 Jun 2;356(6341):968-972. d

    Insert

    PIK3C2B (PIK3C2B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    siRNA resistance to siRNA#2 with 4 silent mutatio…

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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