We narrowed to 2,603 results for: ubiquitin

1. pRGEB32-CrActin-UBI

   Plasmid

   #226709

   Purpose

   For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

   Depositor

   Fenglin Deng

   Article

   Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

   Insert

   CrActin
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSJ982

   Plasmid

   #176622

   Purpose

   Express yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

   Depositor

   Alexander Varshavsky

   Article

   Dong et al Proc Natl Acad Sci U S A. 2020 Jun 23;11

   Insert

   Ub-MPGLW-Fbp1
   

   Use

   Tags

   Ubiquitin N-terminal fusion and triple flag tag C…

   Expression

   Yeast

   Mutation

   Changed first 4 N-terminal amino acid residues of…

   Promoter

   TDH3tc3

   Available since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. LjUBQ:MtGLV1:T35s

   Plasmid

   #164738

   Purpose

   Overexpression of Peptide coding gene (Binary vector)

   Depositor

   Wolf Scheible

   Article

   Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

   Insert

   Medtr6g043230.1
   

   Use

   Tags

   Terminator 35S

   Expression

   Plant

   Mutation

   Promoter

   Lotus japonicus Ubiquitin

   Available since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. LjUBQ:MtGLV9:T35s

   Plasmid

   #164742

   Purpose

   Overexpression of Peptide coding gene (Binary vector)

   Depositor

   Wolf Scheible

   Article

   Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

   Insert

   Medtr4g116360.1
   

   Use

   Tags

   Terminator 35S

   Expression

   Plant

   Mutation

   Promoter

   Lotus japonicus Ubiquitin

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. LjUBQ:MtGLV10:T35s

   Plasmid

   #164739

   Purpose

   Overexpression of Peptide coding gene (Binary vector)

   Depositor

   Wolf Scheible

   Article

   Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

   Insert

   Medtr8g446520.1
   

   Use

   Tags

   Terminator 35S

   Expression

   Plant

   Mutation

   Promoter

   Lotus japonicus Ubiquitin

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG

   Plasmid

   #169958

   Purpose

   Mammalian expression of the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   FLAG and Ub

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE NFkB-TA-LUC-UBC-GFP-W

   Plasmid

   #49343

   Purpose

   pHAGE NFkB-TA-LUC-UBC-GFP-W

   Depositor

   Darrell Kotton

   Article

   Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

   Inserts

   eGFP (eGFP )
   Luciferase
   NFkB consensus binding sequence
   TA
   UBC
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2K7-bsd-UBI-tagRFP-KDEL

   Plasmid

   #114179

   Purpose

   Lentiviral vector for expression of tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

   Depositor

   Nicolas Demaurex

   Article

   Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

   Insert

   tagRFP-KDEL
   

   Use

   Lentiviral

   Tags

   KDEL retention signal and signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE NFKB-TA-LUC-UBC-dTomato-W

   Plasmid

   #49335

   Purpose

   pHAGE NFKB-TA-LUC-UBC-dTomato-W

   Depositor

   Darrell Kotton

   Article

   Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

   Inserts

   NFkB consensus binding sequence
   TA
   Luciferase
   UBC
   dTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-Ufm1 ∆C3

    Plasmid

    #133866

    Purpose

    Expression of human UFM1 mutant lacking the last three C-terminal residues

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    Ubiquitin-fold modifier 1 lacking last three C-terminal residues (UFM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4-NHK-MYC-His6

    Plasmid

    #185350

    Purpose

    Assessing of ubiquitination in cells

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    a1-antitrypsin Hong Kong Null mutant
    

    Use

    Tags

    Myc, 6xHis

    Expression

    Mammalian

    Mutation

    NHK mutant

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. iYFP-Parkin Plasmid

    Plasmid

    #197107

    Purpose

    Expresses YFP-tagged Parkin when induced by Doxycycline in mammalian cells.

    Depositor

    Dori Woods

    Article

    Capelluto et al Adv Biol (Weinh). 2023 Jan 18:e2200246.

    Insert

    parkinson protein 2, E3 ubiquitin protein ligase (PRKN Human)
    

    Use

    Retroviral

    Tags

    YFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-dCas9-KRAB-PGK-HygR

    Plasmid

    #83890

    Purpose

    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Human Ubiquitin C Promoter and mouse phosphoglyce…

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ141-ZmUbi-RZ-Lb

    Plasmid

    #86197

    Purpose

    LbCpf1 Gateway gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    gRNA cloning site
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Maize ubiquitin 1

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-UBC-ERT2-iCre-ERT2

    Plasmid

    #178060

    Purpose

    Conditionally active form of Cre recombinase (activated in response to tamoxifen) under UBC promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ERT2-iCre-ERT2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKb-TnpB1

    Plasmid

    #215333

    Purpose

    ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site

    Depositor

    Kutubuddin Molla

    Article

    Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10

    Insert

    Rice codon optimized ISDra2 TnpB
    

    Use

    Tags

    Not applicable

    Expression

    Plant

    Mutation

    Promoter

    Rice ubiquitin promoter for TnpB and OsU3 promote…

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. nCas9-PPE

    Plasmid

    #140445

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

    Insert

    nCas9(H840A)-M-MLV
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    H840A for Cas9; D200N, T306K, W313F, T330P and L…

    Promoter

    maize Ubiquitin-1

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOD1988-epiDEG

    Plasmid

    #89357

    Purpose

    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pdpy-7 (epidermis specific).

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Insert

    vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    vhhGFP4 (GFP nanobody)

    Expression

    Mutation

    Promoter

    Pdpy-7

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOD2046-bwmDEG

    Plasmid

    #89367

    Purpose

    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmyo-3 (body wall muscle specific).

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Insert

    vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    vhhGFP4 (GFP nanobody)

    Expression

    Mutation

    Promoter

    Pmyo-3

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. WT USP7 TRAF

    Plasmid

    #227226

    Purpose

    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 62-205

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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