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3XFLAG_GAPDH in pCW57_tTA_Blast

Plasmid

#154211

Purpose

Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of GAPDH

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

GAPDH (GAPDH Human)

Use

Lentiviral

Tags

3X FLAG

Expression

Mammalian

Mutation

Promoter

TRE repeats (dox off)

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Neurod1-3xT7

Plasmid

#200890

Purpose

Inducible lentiviral expression of Neurod1

Depositor

Kai Ge

Article

Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

Insert

Neurod1 (Neurod1 Mouse)

Use

Lentiviral; Doxycycline inducible

Tags

3xT7

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX4T-hLIG3

Plasmid

#81055

Purpose

bacterial expression of human LIG3

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

DNA ligase 3 (LIG3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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HaloTag-WDR5

Plasmid

#200880

Purpose

Mammalian expression of human WDR5 with N-terminal HaloTag fusion

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

HaloTag-WDR5 (WDR5 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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DRH296: FCK-Optopatch2

Plasmid

#51694

Purpose

Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

Optopatch2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR221-YAP1

Plasmid

#79503

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

YAP1 (YAP1 Human)

Use

Multisite gateway entry clone

Tags

FLAG

Expression

Mutation

Promoter

no Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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DNMT3L-dCas9

Plasmid

#126587

Purpose

DNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

Insert

DNMT3L (DNMT3L Human)

Use

CRISPR

Tags

3XFLag-NLS-DNMT3L-dCas9-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pProEx Rap1A(G12C, L96F) CL

Plasmid

#224264

Purpose

Protein purification of Rap1A (G12C, L96F)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rap1A (RAP1A Human)

Use

Tags

Expression

Bacterial

Mutation

G12C, L96F

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pNH605-HSE-YFP

Plasmid

#172131

Purpose

Fluorescent reporter for Hsf1 activity

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

4xHSE-YFP

Use

Vector for genomic integration into the yeast gen…

Tags

Expression

Yeast

Mutation

Promoter

CYC1 core promotor with 4 tandem repeats of the H…

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p47-Strep-HA

Plasmid

#113475

Purpose

Expression of human p47 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

p47 (NSFL1C Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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PET-eSpCas9-plus-NLS-6xHis

Plasmid

#207380

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

eSpCas9-plus

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, R1060A, amino acids 1005-1013 replaced wit…

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_OHu19986C_myc tag

Plasmid

#183174

Purpose

used to make AAV vectors for hsTRPA1 tagged with myc

Depositor

Sreekanth Chalasani

Article

Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

Insert

hsTRPA1 (TRPA1 Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGrin2b

Plasmid

#124851

Purpose

Mutagenesis of Grin2b

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin2b (Grin2b Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-hsp70I:QFGal4-SV40pA

Plasmid

#155120

Purpose

Tol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

hsp70l:QFGal4 (hsp70l Zebrafish, Neurospora crassa, Budding Yeast)

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

hsp70l

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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PET-B-eSpCas9-NLS-6xHis

Plasmid

#207383

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-eSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, K1003A, R1060A, amino acids 1005-1013 repl…

Promoter

T7

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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SpRYc

Plasmid

#175575

Purpose

Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells

Depositor

Joseph Jacobson

Article

Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi:

Insert

SpRYc

Use

Tags

EGFP

Expression

Mammalian

Mutation

PID of SpRY grafted onto the N-terminal domain of…

Promoter

CMV

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-cmv-SpCas9-TadCBEd-BlastR

Plasmid

#193848

Purpose

Express TadCBEd in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-acta1:QFGal4-SV40pA

Plasmid

#155121

Purpose

Tol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

acta1:QFGal4

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

acta1

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pNH605-PACE-YFP

Plasmid

#172002

Purpose

Fluorescent reporter for Rpn4 activity

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

4xPACE-YFP

Use

Vector for genomic integration into the yeast gen…

Tags

Expression

Yeast

Mutation

Promoter

CYC1 core promotor with 4 tandem repeats of Rpn4 …

Available since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEa-V106W

Plasmid

#193838

Purpose

Express TadCBEa-V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDa V106W-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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