We narrowed to 11,225 results for: kars;

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pZ149-pcDNA3-BhCas12b_v4

Plasmid

#122446

Purpose

Expresses BhCas12b_v4 in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

CMV-NLS-BhCas12b_v4-NLS-3xHA

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ECFPEYFP

Plasmid

#24520

Depositor

Liusheng He, Jehonathan Pinthus

Article

He et al Cytometry A. 2003 Oct . 55(2):71-85.

Insert

ECFP, EYFP

Tags

ECFP and EYFP

Expression

Mammalian

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
FepA

Plasmid

#26039

Depositor

Johann Deisenhofer

Article

Buchanan et al Nat Struct Biol. 1999 Jan . 6(1):56-63.

Insert

FepA (fepA E Coli)

Expression

Bacterial

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-cdc2-AF (718)

Plasmid

#39872

Depositor

Jonathon Pines

Article

Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

Insert

Cdc2 (CDK1 Human)

Expression

Mammalian

Mutation

AF = T14A, Y15F

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pME-Cas9

Plasmid

#64237

Purpose

expresses codon optimized Cas9 in zebrafish

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Insert

codon optimized Cas9

Use

CRISPR

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pET22b-csgB

Plasmid

#89582

Purpose

Express csgB curli protein with GS linker and Histaq

Depositor

Urartu Seker

Article

Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

Insert

csgB (csgB )

Tags

3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…

Expression

Bacterial

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCHARGE3

Plasmid

#24658

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

Turbo

Expression

Bacterial

Available Since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

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pU6a:sgRNA(tyr)

Plasmid

#64250

Purpose

expresses sgRNA(tyr) under U6a promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Insert

U6a:sgRNA (tyr)

Use

CRISPR

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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MDH1-PGK-H2Kb2

Plasmid

#17852

Depositor

Chang-Zheng Chen

Article

Li et al Cell. 2007 Apr 6. 129(1):147-61.

Insert

H-2Kb (H2-K1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Wt1

Plasmid

#41082

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Wt1 (-KTS alternative isoform) (Wt1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO + minimal CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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Flag-Rap1b

Plasmid

#118325

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

Insert

Rap1b (RAP1B Bovine)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pZ148-BhCas12b-sgRNA-scaffold

Plasmid

#122448

Purpose

Expresses sgRNA for BhCas12b in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

U6-BhCas12b-sgRNA-scaffold, CMV-mCherry

Expression

Mammalian

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN HA

Plasmid

#188645

Purpose

This is the Lazarou lab's standard retrovirus vector backbone for expression of HA-tag fusions onto the N-terminus of cloned genes

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Type

Empty backbone

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

Plasmid

#205278

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH72

Plasmid

#202487

Purpose

Expresses the nanobody VHH72 with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody VHH72

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-AsCas12j-2

Plasmid

#191655

Purpose

All-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2

Depositor

Fong Tian Wong

Article

Tan et al bioRxiv 2021.10.28.465406

Insert

AsCas12j-2

Expression

Bacterial

Promoter

rpsL(XC)-BbsI

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03

Plasmid

#67137

Purpose

Gateway destination vector for cell-free expression with N-term 8xHis eGFP tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

EGFP and His

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCZGY2727

Plasmid

#193330

Purpose

Site specific insertion using CRISPR/Cas9 editing of C. elegans ChrI

Depositor

Yishi Jin

Article

Blazie et al Elife. 2021 Jul 29;10. pii: 68336. doi:

Insert

Hygromycin resistance

Expression

Worm

Promoter

Prps-0

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Stu2 1-306

Plasmid

#38315

Depositor

Anthony Hyman

Article

Widlund et al Mol Biol Cell. 2012 Sep 19.

Insert

5' 918 bp from STU2 ORF (STU2 Budding Yeast)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pMJ333: pU6-MmeFz2_omegaRNA-entry

Plasmid

#205267

Purpose

Human expression vector for MmeFz2 omegaRNA (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

MmeFz2 omegaRNA

Expression

Mammalian

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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