We narrowed to 3,040 results for: multiplex

1. pSLQ10715

   Plasmid

   #183960

   Purpose

   U6-crOct4-CAG-hyperdCas12a-miniVPR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Inserts

   HyperdCas12a
   crRNA targeting Oct4
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D832A, D156R, E292R, D235R, D350R

   Promoter

   CAG and U6

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

   Plasmid

   #104571

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   human codon optimized ‘dead’ Cpf1 fused to four DmrA domains
   

   Use

   CRISPR

   Tags

   NLS-3xHA-3xFLAG-4xDmrA

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. GPR77-DuET

   Plasmid

   #213291

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR77 (C5AR2 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FSHR-DuET

   Plasmid

   #213242

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   FSHR (FSHR Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CYSLTR1-DuET

   Plasmid

   #213225

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CYSLTR1 (CYSLTR1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GPR146-DuET

   Plasmid

   #213265

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR146 (GPR146 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ADORA2A-DuET

   Plasmid

   #213174

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   ADORA2A (ADORA2A Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Anti-MMACHC [N230/21R]

   Plasmid

   #114555

   Purpose

   Mammalian Expression Plasmid of anti-MMACHC (Human). Derived from hybridoma N230/21.

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-MMACHC (Homo sapiens) recombinant mouse monoclonal antibody (MMACHC Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   dual CMV

   Available since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CX3C1-DuET

   Plasmid

   #213217

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CX3C1 (CX3CR1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GPRC5B-DuET

    Plasmid

    #213296

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPRC5B (GPRC5B Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pY31

    Plasmid

    #84746

    Purpose

    Expresses huLbCpf1-P2A-puro and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    puromycin resistance gene
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode

    Plasmid

    #170220

    Purpose

    Barcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CDK1 (CDK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    T14A, Y15F

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUCmini-iCAP-PHP.V2

    Plasmid

    #127848

    Purpose

    non-standard AAV2 rep-AAV-PHP.V2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

    Insert

    Synthetic construct isolate AAV-PHP.V2 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. OPRK1-DuET

    Plasmid

    #213359

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    OPRK1 (OPRK1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pY095

    Plasmid

    #84744

    Purpose

    Expresses huLbCpf1-T2A-GFP and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    GFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUCmini-iCAP-PHP.B4

    Plasmid

    #127849

    Purpose

    non-standard AAV2 rep-AAV-PHP.B4 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

    Insert

    Synthetic construct isolate AAV-PHP.B4 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ADORA1-DuET

    Plasmid

    #213173

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    ADORA1 (ADORA1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MultiMate 5 colors

    Plasmid

    #206266

    Purpose

    Expression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    H2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)
    

    Use

    Recombinant baculovirus production, cre acceptor…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Anti-Shank3 [N69/46R]

    Plasmid

    #140078

    Purpose

    Mammalian Expression Plasmid of anti-Shank3 (Rat). Derived from hybridoma N69/46.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Shank3 (Rat) recombinant mouse monoclonal antibody (Shank3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLQ10713

    Plasmid

    #183958

    Purpose

    U6-crSox2-CAG-hyperdCas12a-miniVPR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Inserts

    HyperdCas12a
    crRNA targeting Sox2
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D832A, D156R, E292R, D235R, D350R

    Promoter

    CAG and U6

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge