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pZS222

Plasmid

#177644

Purpose

In vitro transcription of Patiria miniata Lamp1

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Lamp1

Use

In vitro transcription

Tags

3xGFP

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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CAG.MCAK-mCherry-eDHFR

Plasmid

#176204

Purpose

MCAK (179-583) recruitment responsible for targeted microtubule depolymerization using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

MCAK-mCherry-eDHFR

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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3919-32

Plasmid

#176496

Purpose

pCAG-puro-T2A-TetON3G-polyA

Depositor

Albert La Spada

Article

Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

Insert

puroR-T2A-TetOn3G

Expression

Mammalian

Promoter

TRE

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pZS218

Plasmid

#177641

Purpose

In vitro transcription of Patiria miniata Rab7

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Rab7

Use

In vitro transcription

Tags

mCherry

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pZS230

Plasmid

#177645

Purpose

Bacterial expression of Patiria miniata Dishevelled 1-90

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled 1-90

Tags

GST

Expression

Bacterial

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pZS202

Plasmid

#177638

Purpose

In vitro transcription of Patiria miniata Frizzled 1

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Frizzled 1

Use

In vitro transcription

Tags

GFP

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pJH806_pJH_LldR_Pcon-sfGFP.1TAG

Plasmid

#163737

Purpose

Constitutive expression of green fluorescent protein encoding one stop codon

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.1TAG

Expression

Bacterial

Mutation

gfp.N39TAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH28_pBad_AraC_Para-sfGFP.3TAG

Plasmid

#163723

Purpose

Arabinose-inducible expression of green fluorescent protein encoding three amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC gfp.3TAG

Expression

Bacterial

Mutation

gfp.N39TAG,N135TAG,Y151TAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH626_pJH_LldR_PlldR-sfGFP.1TAG

Plasmid

#163725

Purpose

Lactate-inducible expression of green fluorescent protein encoding one amber codon

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.1TAG

Expression

Bacterial

Mutation

gfp.N39TAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH627_pJH_LldR_PlldR-sfGFP.2TAG

Plasmid

#163726

Purpose

Lactate-inducible expression of green fluorescent protein encoding two amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.2TAG

Expression

Bacterial

Mutation

gfp.N39TAG,Y135TAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH721_pJH_LldR_PlldR-sfGFP.20TAG

Plasmid

#163729

Purpose

Lactate-inducible expression of green fluorescent protein encoding twenty amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.20TAG

Expression

Bacterial

Mutation

gfp with all 20 leucine codons mutated to amber

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH727_pJH_LldR_PlldR-sfGFP.10TAG

Plasmid

#163731

Purpose

Lactate-inducible expression of green fluorescent protein encoding ten amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.10TAG

Expression

Bacterial

Mutation

gfp with first 10 leucine codons mutated to amber

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH774_pJH_LldR_PlldR-sfGFP.1opal

Plasmid

#163733

Purpose

Lactate-inducible expression of green fluorescent protein encoding one opal codon

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.1TGA

Expression

Bacterial

Mutation

gfp with first leucine codon mutated to amber

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHIS8-3_DL121

Plasmid

#171953

Purpose

Expression vector for ecDHFR-asLOV2 fusion protein. Also has TYMS

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Insert

Fusion of ecDHFR and asLOV2 at position 121 of DHFR

Tags

8-His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2 Bighead S-HA

Plasmid

#172485

Purpose

for synthesis of mRNA for injection into xenopus laevis embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

Bighead.S

Use

In vitro mrna synthesis

Tags

HA and S-HA

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-xFAM3b.L-HA

Plasmid

#172852

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Proc Natl Acad Sci U S A. 2018 Sep 25;11

Insert

xFAM3B.L (fam3b.L Frog)

Tags

HA

Expression

Mammalian

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_DL121_WTTS

Plasmid

#171954

Purpose

Selection vector for chimeric fusion of ecDHFR and asLOV2.

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Inserts

Fusion of ecDHFR and asLOV2 at position 121 of DHFR
Thymidylate Synthase

Tags

8-His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET-11a HIV-1 P6

Plasmid

#154201

Purpose

Bacterial expression of Gag P6 from HIV-1 HXB2 strain

Depositor

Lalit Deshmukh

Article

Ramaraju et al Biochemistry. 2021 Aug 3. doi: 10.1021/a

Insert

Gag P6 protein from HIV-1 clone HXB2

Tags

GB1-6xHis Tag

Expression

Bacterial

Promoter

T7

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm3_A

Plasmid

#145888

Purpose

Insect Expression of DmLSm3

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm3 (LSm3 Fly)

Expression

Insect

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-2xStrep-mu68

Plasmid

#162662

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged mu68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

mu68

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68

Plasmid

#162625

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-mu68

Plasmid

#162627

Purpose

Expresses N-terminal 2x-Strep-tagged mu68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

mu68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-UL52

Plasmid

#162628

Purpose

Expresses N-terminal 2x-Strep-tagged UL52 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL52

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-2xStrep-UL32

Plasmid

#162629

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
HRP-1LC

Plasmid

#139116

Purpose

express the LC of HRP-1

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Bacterial

Mutation

181-346

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-pol1-N1

Plasmid

#160988

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Tags

GST

Expression

Bacterial

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGST-pol1-N2-2A2

Plasmid

#160991

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Tags

GST

Expression

Bacterial

Mutation

F58A/D62A

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGST-pol1-N2

Plasmid

#160989

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Tags

GST

Expression

Bacterial

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGST-pol1-N1-2A2

Plasmid

#160990

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Tags

GST

Expression

Bacterial

Mutation

F58A/D62A

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p5343_UC57

Plasmid

#126645

Purpose

environmental plasmid p5343 cloned into pUC57

Depositor

Aindrila Mukhopadhyay

Article

Kothari et al mSystems. 2019 Dec 17;4(6). pii: 4/6/e00

Insert

Environmental plasmid p5343

Use

Synthetic Biology

Promoter

NA

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+ AMIC

Plasmid

#118420

Purpose

For mammalian expression of Amoeba myosin 1C

Depositor

Michael Tsang

Article

Saydmohammed et al Nat Commun. 2018 Aug 23;9(1):3381. doi:

Insert

Amoeba myosin 1C

Expression

Mammalian

Mutation

See depositor comments below

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
S11_EcoRV_CAMKIIA_AscI

Plasmid

#128475

Purpose

CAMKII1 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

CAMKII1 promoter

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
S10_EcoRV_hcSYN_AscI

Plasmid

#128474

Purpose

hcSYN promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

hcSYN promoter

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pALPS puro miR30-TAK1

Plasmid

#117162

Purpose

Target site AGCGCCCTTCAATGGAGGAAAT

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pALPS puro miR30-STAT2

Plasmid

#117161

Purpose

Target site TTTAAGTTCCACAGACTTGGA

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS406 ATG9-GFP T119D

Plasmid

#114668

Purpose

Expresses yeast ATG9 (T119D) under the endogenous promoter with a GFP C-terminal tag

Depositor

Daniel Klionsky

Article

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Promoter

ATG9

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKN3097

Plasmid

#113392

Purpose

Galactose-inducible Ty3 with extra PPT- used for random bar code library

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Insert

Ty3

Expression

Yeast

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2-lyzC-RFP-miR-30 backbone

Plasmid

#97147

Purpose

As a control of miRNA expression

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Type

Empty backbone

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7T3D-PacI-Tmeff2

Plasmid

#99728

Purpose

to make in situ RNA probes for Tmeff2

Depositor

Mengsheng Qiu

Article

Huang et al Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1

Insert

Tmeff2 (Tmeff2 Mouse)

Use

In situ probe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP1071-2

Plasmid

#99502

Purpose

3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3utr::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3’utr::linker::TEV

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GAL-MAGE-2-429

Plasmid

#89126

Purpose

mammalian expression of full-length human MAGE-A11 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Tags

GAL4 DBD

Expression

Mammalian

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
VP-MAGE-2-429

Plasmid

#89125

Purpose

mammalian expression of full-length human MAGE-A11 with N-terminal VP16 activation domain

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Tags

VP16

Expression

Mammalian

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
pEN_TTmiRc2 3XFLAG-EGFP-NLS

Plasmid

#87778

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

eGFP

Tags

3xFLAG and NLS

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TTmiRc2 3XFLAG-MKK7-EE-NLS

Plasmid

#87777

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK7-EE

Tags

3xFLAG and NLS

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mCBFKOgRNA5

Plasmid

#84366

Purpose

Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

Cbfa2t2-gRNA5

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
mCBFKOgRNA2

Plasmid

#84365

Purpose

Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

Cbfa2t2-gRNA2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pHU6_Ch5_155183064-gRNA-for

Plasmid

#81214

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch5 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDZ584 pKAN 1x-mCherry

Plasmid

#73172

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 1x-mCherry

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

1x-mCherry

Use

Unspecified

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
His10-PS-SNAPf-9R (XSB525)

Plasmid

#78513

Purpose

Construct for expressing His10 - SNAPf -9R in E. Coli

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

SNAPf

Tags

His10 followed by a prescission protease claevage…

Expression

Bacterial

Mutation

9R added to the C-terminal of SNAPf

Available Since

June 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-Cox15-13xMyc

Plasmid

#61721

Purpose

yeast Cox15 heme A synthase with C-terminal 13xMyc tag in low-copy yeast expression vector

Depositor

Oleh Khalimonchuk

Article

Swenson et al J Biol Chem. 2016 Mar 3. pii: jbc.M115.7

Insert

Cox15 (COX15 Budding Yeast)

Tags

13xMyc

Expression

Yeast

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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We narrowed to 22,938 results for: Tes