We narrowed to 24,482 results for: Spr

1. phU6-gRNA

   Plasmid

   #53188

   Purpose

   Expresses the S. pyogenes sgRNA from the human U6 promoter

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. phH1-gRNA

   Plasmid

   #53186

   Purpose

   Expresses the S. pyogenes sgRNA from the H1 promoter

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   human H1

   Available Since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmU6-gRNA

   Plasmid

   #53187

   Purpose

   Expresses the S. pyogenes sgRNA from the mouse U6 promoter

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. p426-SNR52p-gRNA.CAN1.Y-SUP4t

   Plasmid

   #43803

   Purpose

   Encodes a gRNA that targets Can1.Y in yeast.

   Depositor

   George Church

   Article

   Dicarlo et al Nucleic Acids Res

   Insert

   CAN1.y gRNA (CAN1 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   March 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV GG hUbC-dsRED

   Plasmid

   #84034

   Purpose

   Contains Golden Gate cassette to express up to four gRNAs and dsRED

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMA7CR_2.0

   Plasmid

   #73950

   Purpose

   pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX

   Depositor

   Alex Nielsen

   Article

   Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

   Inserts

   cas9
   lambda beta
   dam
   recX
   

   Expression

   Bacterial

   Promoter

   pAra and pTet

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCVpuro-Cas9-EGFP

   Plasmid

   #159126

   Purpose

   Expresses active Cas9 fused with EGFP for fluorescence microscopy

   Depositor

   Taekjip Ha

   Article

   Liu et al Science. 2020 Jun 12;368(6496):1265-1269

   Insert

   Cas9 fused to EGFP
   

   Use

   CRISPR and Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   MSCV LTR promoter

   Available Since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-gRNA

   Plasmid

   #46759

   Purpose

   gRNA cloning vector for in vitro transcription of target gRNA

   Depositor

   Wenbiao Chen

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   gRNA core
   

   Use

   CRISPR; Zebrafish expression

   Promoter

   T7

   Available Since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJJB308

   Plasmid

   #107699

   Purpose

   AtU6 Promoter-LwaCas13a-gRNA Scaffold

   Depositor

   Daniel Voytas

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJJB296

    Plasmid

    #107691

    Purpose

    35SPromoter-LwaCas13a-HSPTerminator

    Depositor

    Daniel Voytas

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    Cas13a
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. U6.3>Control.gRNA.f+e

    Plasmid

    #99140

    Purpose

    Control gRNA under the regulation of an optimized chicken specific U6 promoter

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

    Insert

    Control gRNA
    

    Use

    CRISPR

    Promoter

    Chicken U6.3

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDonor_sU6

    Plasmid

    #69351

    Purpose

    sgRNA scaffold and synthetic U6 promoter

    Depositor

    Andrea Ventura

    Article

    Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

    Insert

    sU6 promoter and sgRNA scaffold flanked by BbsI sites
    

    Use

    CRISPR

    Promoter

    synthetic U6 promoter

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDonor_mU6

    Plasmid

    #69350

    Purpose

    sgRNA scaffold and mouse U6 promoter

    Depositor

    Andrea Ventura

    Article

    Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

    Insert

    mU6 promoter and sgRNA scaffold flanked by BbsI sites
    

    Use

    CRISPR

    Promoter

    mU6 promoter

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA

    Plasmid

    #99698

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAV

    Depositor

    George Church

    Type

    Empty backbone

    Use

    AAV

    Available Since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pColE1_sgRNA_pos6_AmpR

    Plasmid

    #119149

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSET-dCas9

    Plasmid

    #110183

    Purpose

    Expression of the dCas9 gene in Streptomyces

    Depositor

    Yinhua Lu

    Article

    Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

    Insert

    dCas9
    

    Use

    CRISPR; I

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    ermE*p

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pColE1_sgRNA_NT_AmpR

    Plasmid

    #119144

    Purpose

    Encodes non-targeting sgRNA

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    non-targeting sgRNA
    

    Use

    Crispr

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAH01Sp::Cas9

    Plasmid

    #128160

    Purpose

    Ptet-controlled expression of S. pyogenes Cas9

    Depositor

    Calvin Henard

    Article

    Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

    Insert

    Sp Cas9
    

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJA58

    Plasmid

    #59933

    Purpose

    gRNA for cleavage at dpy-10(cn64) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    dpy-10(cn64) gRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pColE1_sgRNA_pos8_AmpR

    Plasmid

    #119151

    Purpose

    Encodes sgRNA targeting position 8 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 8 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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