We narrowed to 12,677 results for: sequence

1. pOpen-XbaIR

   Plasmid

   #165567

   Purpose

   Type II restriction enzyme that recognizes the double-stranded sequence TCTAGA and cleaves after T-1.

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   XbaI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pMSCV2.2-IRES- GFP-Opa1

   Plasmid

   #191971

   Purpose

   Plasmid for cloning reaction, containing OPA1 coding sequence.

   Depositor

   Luca Scorrano

   Insert

   GFP-Opa1
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-Flex-Rev-3xGFP

   Plasmid

   #122500

   Purpose

   Cre dependent GFP expression.

   Depositor

   Kimberly Ritola

   Insert

   3x GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   each GFP has unique nt sequence

   Promoter

   Available since

   Jan. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOpen-NcoIR

   Plasmid

   #165568

   Purpose

   Type II restriction enzyme that recognizes the double-stranded sequence CCATGG and cleaves after C-1.

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   NcoI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pOpen-PstIM

   Plasmid

   #165536

   Purpose

   Methylase that recognizes the dsDNA sequence CTGCAG, methylates on A-5 on both strands, and protects DNA from cleavage by PstI.

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   M.PstI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. hU6-Sa_tracr

   Plasmid

   #135973

   Purpose

   Contains tracrRNA-hU6 sequence for double sgRNA cloning

   Depositor

   Yongming Wang

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-MTS-EGFP-V5

   Plasmid

   #200816

   Purpose

   expresses MTS-EGFP-V5 in mammalian cells

   Depositor

   Hung-Chun Chang

   Insert

   EGFP
   

   Use

   Tags

   V5 and mitochondrial targeting sequence (MTS)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM-11-DmPABPC1_501-634_J

   Plasmid

   #146641

   Purpose

   Bacterial Expression of HsPABPC1_501-634

   Depositor

   Elisa Izaurralde

   Insert

   HsPABPC1_501-634
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   2 silent mutations compared to the sequence given…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1B-lambdaN-HA-HsTNRC6C_G

   Plasmid

   #146374

   Purpose

   Insect Expression of HsTRC6C

   Depositor

   Elisa Izaurralde

   Insert

   HsTRC6C
   

   Use

   Tags

   Expression

   Insect

   Mutation

   two silent mutations C2349T and A2547C compared t…

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-Opa1

    Plasmid

    #191961

    Purpose

    Gateway Destination Vector, containing OPA1 coding sequence, for Gateway Cloning Technology

    Depositor

    Luca Scorrano

    Insert

    Opa1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOME0276M

    Plasmid

    #182614

    Purpose

    Reversed sequence of Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome. TO_7 Control for pOME0275

    Depositor

    Dirk Dittmer

    Insert

    Reverse compliment HHV8 TO_7 control
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOME0237M

    Plasmid

    #182599

    Purpose

    Reverse complement sequence of anti-PAN pOME0236 Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.

    Depositor

    Dirk Dittmer

    Insert

    Reverse compliment of anti-PAN (pOME0236)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGF-bio-His

    Plasmid

    #53340

    Purpose

    Expresses full-length Pro-epidermal growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    EGF (EGF Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiGuide

    Plasmid

    #117986

    Purpose

    pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.

    Depositor

    Paul Khavari

    Article

    Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31

    Insert

    sgRNA backbone (F+E)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. CTGF-bio-His

    Plasmid

    #52028

    Purpose

    Expresses full-length Connective tissue growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CTGF (CCN2 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. EFNB1-bio-His

    Plasmid

    #51749

    Purpose

    Expresses full-length Ephrin-B1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    EFNB1 (EFNB1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ESAM-bio-His

    Plasmid

    #51751

    Purpose

    Expresses full-length Endothelial cell-selective adhesion molecule precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ESAM (ESAM Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOPINE GFP nanobody:Halo:His6

    Plasmid

    #111090

    Purpose

    Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

    Depositor

    Lennart Wirthmueller

    Article

    Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

    Insert

    GFP Nanobody
    

    Use

    Tags

    Halo and His6

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7 promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. FASTHDR-tRNApromo-sgRNA-espCas91_1

    Plasmid

    #167210

    Purpose

    Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. FURIN-bio-His

    Plasmid

    #51755

    Purpose

    Expresses full-length Furin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    FURIN (FURIN Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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