We narrowed to 7,151 results for: CIT
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CamKII-LGI1-pHmScarlet
Plasmid#185538PurposeExpresses LGI1-pHmScarlet under the CamKII promoterDepositorInsertLGI1 (Lgi1 Rat)
UseLentiviralTagspHmScarletExpressionMammalianMutationPromoterCamKIIAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
CamKII-ADAM23-pHluorin
Plasmid#185539PurposeExpresses ADAM23-pHluorin under the CamKII promoterDepositorInsertADAM23 (Adam23 Rat)
UseLentiviralTagspHluorinExpressionMammalianMutationPromoterCamKIIAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 P35S:dCas9:TV:Tnos (GB2047)
Plasmid#160626PurposeTU for the constitutive expression of dCas9 fused to TV (TALx6 - VP128) activation domainsDepositorInsertP35s-dCas9:TV-Tnos
UseTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GSDMD-eGFP-SV40p-BlasR
Plasmid#228254PurposepLenti-CMV-GSDMD-eGFP-SV40p-BlasR plasmid for ectopic expression of human full-length GSDMD fusion with GFPDepositorInsertGSDMD (GSDMD Human)
UseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ultra-Exon1Q23-Myc-A
Plasmid#110486PurposeLentiviral vector to express wild-type N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged wild type huntingtin exon1 fragment with 23 repeats of Q)DepositorInsertHomo sapiens huntingtin (HTT Human)
UseLentiviralTagsMycExpressionMammalianMutationShort 3'UTR wild type huntingtin exon1 fragm…PromoterUbCAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
dCas12Max-ABE
Plasmid#195335Purposevector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized dCas12Max-ABE
UseTagsExpressionMammalianMutationN243R, E336R, D1049APromoterCBh, CMV, hU6Available sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 SIRT1-FL
Plasmid#105670PurposeMammalian expression construct of mouse SIRT1 full length isoformDepositorInsertSIRT1 (Sirt1 Mouse)
UseTagsExpressionMammalianMutationSynonymous base changes not affecting the protein…PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Chronos-GFP
Plasmid#99232PurposeAAV-mediated expression of Chronos-GFP under the CAG promoter. Using bGHpA signal.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterCAGAvailable sinceDec. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET42b-BE3
Plasmid#87437PurposeExpresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorInsertBE3 (Apobec1 Bacillus phage PBS2, Rat, Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pESF140_HisSpy_DDB1∆B
Plasmid#124213PurposeInsect cell expression vector for His6, Spy tagged hsDDB1∆BDepositorInsertDNA damage-binding protein 1 (DDB1 Human)
UseTagsHis6-SpyExpressionInsectMutationinternal deletion of BPB domain to aid crystalliz…PromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-PSD95-EGFP-WPRE
Plasmid#133785PurposeCre-dependent EGFP Fluorescent reporter for overexpressed postsynaptic marker PSD95 in mammalian cellDepositorInsertPSD95-EGFP (Dlg4 Rat)
UseAAV and Cre/LoxTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET42b-HF-BE3
Plasmid#87438PurposeExpresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorInsertHF-BE3 (Apobec1 Bacillus phage PBS2, Rat, Human)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCRY1 HA tag
Plasmid#110298Purposefull length wt mouse Cry1 HA tag in pcDNA3.1DepositorInsertwt mcry1 HA tag (Cry1 Mouse)
UseTagsHA tagExpressionMammalianMutationwildtypePromoterCMVAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Chronos-GFP
Plasmid#59170PurposeAAV-mediated expression of Chronos-GFP under the Syn promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV5InsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterSynAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
dCas12Max-CBE
Plasmid#195337Purposevector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized dCas12Max-CBE
UseTagsExpressionMammalianMutationN243R, E336R, D1049APromoterCBh, CMV, hU6Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Exon1Q23-Myc-B
Plasmid#110488PurposeLentiviral vector to express wild-type N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged wild-type huntingtin exon1 fragment with 23 repeats of Q)DepositorInsertHomo sapiens huntingtin (HTT Human)
UseLentiviralTagsMycExpressionMammalianMutationshort 3'UTR mutant huntingtin exon1 fragment…PromoterUbCAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
CamKII-LGI1-pHluorin
Plasmid#185537PurposeExpresses LGI1-pHluorin under the CamKII promoterDepositorInsertLGI1 (Lgi1 Rat)
UseLentiviralTagspHluorinExpressionMammalianMutationPromoterCamKIIAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cas12Max
Plasmid#195334Purposevector for encoding a human codon-optimized Cas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized Cas12Max
UseTags3xFlagExpressionMammalianMutationN243RPromoterCBh, CMV, hU6Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode
Plasmid#170220PurposeBarcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCDK1 (CDK1 Human)
UseLentiviralTagsExpressionMutationT14A, Y15FPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Exon1Q145-Myc-B
Plasmid#110489PurposeLentiviral vector to express mutant N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)DepositorInsertHomo sapiens huntingtin (HTT Human)
UseLentiviralTagsMycExpressionMammalianMutationpartial long 3'UTR wild type huntingtin exon…PromoterUbCAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P2-p53 (1-90) (72R)
Plasmid#62081PurposeExpression of human p53 (TAD residues 1-90) (72R) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsGSTExpressionBacterialMutationContains TAD/PP residues 1-90, 72RPromotertacAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ai66(RCRL-tdT) targeting vector
Plasmid#61578PurposeTarget a Dre and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locusDepositorInsertCAG-RSR-LSL-tdTomato
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterCAGAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV pTRE-FLEX-EGFP-WPRE-bGHpA
Plasmid#65449PurposeCan be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent mannerDepositorInsertEGFP
UseAAVTagsExpressionMutationPromotertetOAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HF-BE3
Plasmid#87439PurposeExpresses HF-BE3 with C-terminal NLS in mammalian cellsDepositorInsertHF-BE3 (Apobec1 Bacillus phage PBS2, Rat, Human)
UseTagsExpressionMammalianMutationMammalian codon-optimized Cas9-HFPromoterCMVAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-Chrimson-GFP
Plasmid#62718PurposeAAV-mediated expression of Chrimson-GFP under the CaMKII promoter. Using SV40pA signal.DepositorInsertChrimson-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterCaMKIIAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-tdTomato
Plasmid#122101PurposeAAV-mediated expression of tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.DepositorInserttdTomato
UseAAVTagsNAExpressionMammalianMutationPromoterEF1a（1.1kb short version)Available sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D
Plasmid#169912PurposeExpression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.DepositorInsertdCjCas9
UseCRISPRTags10XGCN4, 2xSV40NLS, and SV40NLS-HAExpressionMammalianMutationD8A, H559A in cjCas9PromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF019 MBP-β-catenin-His
Plasmid#154070PurposeExpresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) in E.coliDepositorInsertCTNNB1 (CTNNB1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLEX-fwd[Chrimson-tdT]
Plasmid#59137PurposeExpression vector for Chrimson-tdTomato in a floxed/forward-reading, Cre-dependent manner (Cre turns gene off)DepositorInsertChrimson-tdTomato
UseCre/LoxTagstdTomatoExpressionMammalianMutationPromoterCAGAvailable sinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only
