We narrowed to 7,412 results for: CIT

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pD2sE_SC10

Plasmid

#219716

Purpose

DV2 stable E dimer with fusion loop mutation

Depositor

Brian Kuhlman

Article

Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

Insert

DV2 sE SC10

Tags

8xHis and human serum albumin (HSA)

Expression

Mammalian

Mutation

G106D, A259W, T262R, F279W, T280P

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2-FUS-1-413aa-V5

Plasmid

#29612

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-413aa (FUS Human)

Tags

V5

Expression

Mammalian

Mutation

Contains FUS aa# 1-413

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pLC-CUL4A K705R-P2A-Puro

Plasmid

#124305

Purpose

Lentiviral vector for expression of Nedd8 attachment mutant K705R CUL4A-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

CUL4A (CUL4A Human)

Use

Lentiviral

Expression

Mammalian

Mutation

changed Lysine 705 to Arginine

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnk9-IRES-EGFP

Plasmid

#164949

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnk9 and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnk9 (kcnk9 Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Promoter

actinb (actb2) promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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3I91

Plasmid

#62514

Purpose

Bacterial expression plasmid for use in structural determination.

Depositor

Cheryl Arrowsmith

Article

Kaustov et al J Biol Chem. 2011 Jan 7;286(1):521-9. do

Insert

CBX8 (CBX8 Human)

Tags

6xHis and TEV cleavage

Expression

Bacterial

Promoter

T7

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA 3.2-FUS-1-269aa-V5

Plasmid

#29610

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-269aa (FUS Human)

Tags

V5

Expression

Mammalian

Mutation

Contains FUS aa# 1-269

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pLV-shEsr2

Plasmid

#120722

Purpose

Lentiviral vector that expresses GFP and an shRNA targeting Esr2 (in pLL3.7).

Depositor

Amy Lasek

Article

Vandegrift et al J Neurosci. 2020 Jul 1;40(27):5196-5207.

Insert

Esr2 (Esr2 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Promoter

mouse U6

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2-FUS-1-373aa-V5

Plasmid

#29611

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-373aa (FUS Human)

Tags

V5

Expression

Mammalian

Mutation

Contains FUS aa# 1-373

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pLenLox_U6 Nlgn2

Plasmid

#59358

Purpose

Lentiviral expression vector: Neuroligin 2 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

Depositor

Peter Scheiffele

Article

Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

Inserts

Neuroligin 2 shRNA (rat and mouse) (Nlgn2, Nlgn2 Mouse, Rat)
eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG and mouse U6

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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416Gal-FUS-R521G-YFP

Plasmid

#29623

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

R521G

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pET24a-ncOGT-TPR

Plasmid

#190822

Purpose

For expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

O-GlcNAc Transferase (OGT Human)

Tags

6x His

Expression

Bacterial

Mutation

C467X. Also codon optimized for expression in E.c…

Promoter

T7

Available Since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-EGFP.T2A.NCLX

Plasmid

#181873

Purpose

Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter

Depositor

Hilmar Bading

Article

Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

Inserts

solute carrier family 8 member B1 (Slc8a1 Mouse)
EGFP

Use

AAV and Adenoviral

Tags

HA and myc

Expression

Mammalian

Promoter

synthetic hybrid CAG promoter and synthetic hybri…

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pUPD2 sgRNAscaffold 2.1 scRNA (GB1437)

Plasmid

#160571

Purpose

Version of SgRNA scaffold with the sequence 2.1 of Ms2 aptamer in the 3' end.

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNAscaffold 2.1 scRNA

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-CEBPa-WT

Plasmid

#215601

Purpose

For overexpression of Gal4-DBD CEBPa WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa (CEBPA Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CDK1-P2A-Hygro_Barcode

Plasmid

#170221

Purpose

Barcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK1 (CDK1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_LYN-1/1

Plasmid

#91536

Purpose

Protein expression and purification of human SH3 domain construct LYN-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LYN-1/1 (LYN Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTE4495

Plasmid

#80338

Purpose

mammalian expression MbCpf1 nuclease and Mb crRNA

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Mb crRNA
MbCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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416GPD-FUS-YFP

Plasmid

#29594

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Available Since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
pET28-p53 (1-90) (72P)

Plasmid

#62080

Purpose

Expression of human p53 (1-90) (72P) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

contains TAD/PP residues 1-90; 72P

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pDGB3 alpha2 P35S:MS2:EDLL:Tnos (GB1738)

Plasmid

#160622

Purpose

TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain EDLL

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35s_MS2:EDLL_Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET-dSpCas9-HF1-VP64-6xHis

Plasmid

#92118

Purpose

Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64

Use

CRISPR

Tags

3xFLAG, 6xHis, NLS, and VP64

Expression

Bacterial

Mutation

D10A, N497A, R661A, Q695A, H840A, Q926A

Promoter

T7

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-TetON-mEGFP-NGN2_WT

Plasmid

#215610

Purpose

For integration of NGN2 WT T2A mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

NGN2 (NEUROG2 Human)

Tags

T2A mEGFP

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGAP32-1/1

Plasmid

#91312

Purpose

Protein expression and purification of human SH3 domain construct ARHGAP32-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGAP32-1/1 (ARHGAP32 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-CsChR-GFP

Plasmid

#58841

Purpose

AAV expression of CsChR-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CsChR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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GST-TEV-FUS-267-526aa

Plasmid

#29630

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-267-526aa (FUS Human)

Tags

GST and TEV

Expression

Bacterial

Mutation

Contains FUS aa# 267-526

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
GST-TEV-FUS-1-373aa

Plasmid

#29631

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-373aa (FUS Human)

Tags

GST and TEV

Expression

Bacterial

Mutation

Contains FUS aa# 1-373

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pLEX307-SARS-CoV-3CL-C145A

Plasmid

#160281

Purpose

Allows for constituitive mammalian expression of the SARS-CoV 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C145A

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-HCoV-NL63-3CL-C144A

Plasmid

#160287

Purpose

Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease with C144A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C144A

Promoter

EF1a

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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423Gal-FUS-YFP

Plasmid

#29615

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pTE4999

Plasmid

#107555

Purpose

Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hFnCpf1(dead1)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

D917A

Promoter

CMV

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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