We narrowed to 4,521 results for: GCA;

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RPS15A sgRNA-4 lentiCRISPR v2-Blast plasmid

Plasmid

#192229

Purpose

lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-2 lentiCRISPR v2 plasmid

Plasmid

#192232

Purpose

lentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RPS4X sgRNA-1 lentiCRISPR v2 plasmid

Plasmid

#192231

Purpose

lentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNotch2#1/Cre

Plasmid

#193225

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch2 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNotch2#1 (Notch2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgTmem132d#2/Cre

Plasmid

#193243

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Tmem132d gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgTmem132d#2 (Tmem132d Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgZfp536#1/Cre

Plasmid

#193248

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Zfp536 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgZfp536#1 (Zfp536 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSis#1/Cre

Plasmid

#193237

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Sis gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgSis#1 (Sis Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNotch3#2/Cre

Plasmid

#193228

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch3 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNotch3#2 (Notch3 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNotch1#2/Cre

Plasmid

#193224

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNotch1#2 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-dTLN1 intron

Plasmid

#176224

Purpose

A vector for the CRISPR-Cas9 system targeting an intron of dog TLN1 (talin1)

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

A gRNA targeting the dog TLN1 gene and the cDNA of Cas9 (TLN1 )

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G6_T804N_Std_Dual_epegRNA_tevopreQ1

Plasmid

#187457

Purpose

Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G6 T804N pegRNA (ATP1A1 Human)

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRnf43#2/Cre

Plasmid

#173614

Purpose

Expresses a Rnf43-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Rnf43 (Rnf43 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgStag2#1/Cre

Plasmid

#173659

Purpose

Expresses a Stag2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Stag2 (Stag2 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pX458_sgAgo2_Nterm

Plasmid

#170920

Purpose

Expresses spCas9 and sgRNA targeting the N-terminus of Ago2 for N-terminal tagging

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

sgAgo2 (Ago2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-CD45

Plasmid

#175943

Purpose

knockout mouse CD45

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

CD45 gRNA (Ptprc Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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330-cherry-hCYP3A4-enhancer-L-sgRNA

Plasmid

#176840

Purpose

Targeting human CYP3A4 proximal enhancer left boundary. sgRNA expressing cells could be FACS sorted by cherry expression.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Human CYP3A4 proximal enhancer left boundary

Expression

Mammalian

Promoter

U6

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMCB320-sgZmat3.55.9

Plasmid

#169026

Purpose

Expresses an sgRNA targeting Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

sgRNA targeting Zmat3, mCherry, Puromycin resistance

Use

CRISPR, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

mU6

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shKif11

Plasmid

#160962

Purpose

Kif11 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Kif11 shRNA (Kif11 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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BE3-YE1

Plasmid

#132943

Purpose

Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

Depositor

Hui Yang

Article

Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

Insert

BE3-(W90Y-R126E)

Use

CRISPR; Base editor

Expression

Mammalian

Mutation

BE3-(W90Y-R126E)

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-NCOA7g3 (BB24)

Plasmid

#139459

Purpose

Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA FANCD2 Q223Stop

Plasmid

#139332

Purpose

Plasmid expressing a sgRNA to introduce FANCD2 Q223Stop using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert FANCD2 Q223Stop using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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gh258

Plasmid

#106860

Purpose

expression of gRNA targeting ALG3

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ALG3 (ALG3 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh145

Plasmid

#106804

Purpose

expression of gRNA targeting FKRP

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

FKRP (FKRP Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh67

Plasmid

#106738

Purpose

expression of gRNA targeting B3GNT1 (B4GAT1)

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GNT1 (B4GAT1) (B4GAT1 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh17

Plasmid

#106701

Purpose

expression of gRNA targeting GALNT17

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT17 (GALNT17 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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rd

Plasmid

#112912

Purpose

Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag

Depositor

C. James McKnight

Article

Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20

Insert

dematin (DMTN Human)

Tags

Glutatione-S-Tranferase and precision protease si…

Expression

Bacterial

Mutation

PEST sequence near the N-terminal removed. 5′-GCG…

Promoter

T7

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pPN103

Plasmid

#91630

Purpose

Express sgRNA targeting human IMMP2L

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

IMMP2L (IMMP2L Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN088

Plasmid

#91613

Purpose

Express sgRNA targeting human FURIN

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

FURIN (FURIN Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pPN171

Plasmid

#91600

Purpose

Express sgRNA targeting human DLGAP1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DLGAP1 (DLGAP1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN190

Plasmid

#91577

Purpose

Express sgRNA targeting human BRINP2

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

BRINP2 (FAM5B Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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