We narrowed to 17,812 results for: She

1. RV-Syn-Dio-mVenus

   Plasmid

   #153204

   Purpose

   Can be used to generate retrovirus that will mark the membrane (Palmitoylated-Venus) in the presence of Cre from the rat synapsin promoter

   Depositor

   Lynette Lim

   Insert

   membrane-tagged-Venus
   

   Use

   Retroviral

   Promoter

   rat synapsin

   Available Since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET StrepII TEV expression vector with BioBrick polypromoter restriction sites (14-R)

   Plasmid

   #48312

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   StrepII

   Expression

   Bacterial

   Available Since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. GSK-3beta S9A (Xenopus)/pCS2+

   Plasmid

   #16333

   Depositor

   Peter Klein

   Insert

   GSK-3beta (gsk3b.L Frog)
   

   Use

   Vertebrate expression

   Mutation

   GSK-3 with serine-9 to alanine mutation

   Available Since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pLV Tmem9b-V5

   Plasmid

   #175152

   Purpose

   Lentiviral expression of mouse Tmem9b-V5

   Depositor

   Larry Gerace

   Insert

   Tmem9b (Tmem9b Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB6-Hipp11-DTA-Capecchi-rev

   Plasmid

   #127054

   Purpose

   B6J Hipp11 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, reverse cloning sites, low copy number

   Depositor

   Mario Capecchi

   Type

   Empty backbone

   Use

   Mouse Targeting

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH0103 NEDD4L WW domain #4

   Plasmid

   #104207

   Purpose

   Bacterial expression of WW domain #4 from NEDD4L

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   NEDD4L WW domain #4 (NEDD4L Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK367

   Plasmid

   #72406

   Purpose

   Produces Acetobacter aceti 1023 Est06

   Depositor

   T. J. Kappock

   Insert

   lipase
   

   Expression

   Bacterial

   Mutation

   lacks Met1 and Thr2 in annotated (v1) sequence

   Promoter

   T7

   Available Since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-hSPC-TFEBeGFP-W

   Plasmid

   #126692

   Purpose

   Lineage specific over expression of TFEB in lung alveolar epithelial cells

   Depositor

   Darrell Kotton

   Insert

   TFEB (TFEB Human)
   

   Use

   Lentiviral

   Mutation

   Deleted Stop Codon on TFEB

   Promoter

   hSPC

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLK89 - Edit-directing plasmid cloning insert

   Plasmid

   #98846

   Purpose

   Contains the insert used for step 2 of edit-directed plasmid cloning

   Depositor

   Leonid Kruglyak

   Insert

   KanR
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Changed sequence to remove a MluI cut site

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX6P1-Reps1-EH domain

    Plasmid

    #36860

    Depositor

    Yvonne Groemping

    Insert

    Reps1 (REPS1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-225 and 315-769

    Available Since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103 C20orf67 WW domain

    Plasmid

    #104273

    Purpose

    Bacterial expression of WW domain from C20orf67

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    C20orf67 WW domain (PCIF1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-PE2-pegRNA-ST2-com-vector

    Plasmid

    #136272

    Purpose

    Expresses PE2 and ST2-com modified pegRNA cassette

    Depositor

    Baisong Lu

    Insert

    PE2, pegRNA scaffold
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBacI/MDR3cysless

    Plasmid

    #74400

    Purpose

    expression of MDR3 in insect cells

    Depositor

    Robert Nakamoto

    Insert

    MDR3 (Abcb1a Mouse)
    

    Tags

    10His

    Expression

    Insect

    Mutation

    all Cys to Ala

    Promoter

    Polyhedrin

    Available Since

    March 2, 2018

    Availability

    Academic Institutions and Nonprofits only

14. ScSpe1_pET20b(+)

    Plasmid

    #118343

    Purpose

    Expresses the His tagged Ornithine Decarboxylase from Saccharomyces cerevisiae in Escherichia coli BL21 or similar strains

    Depositor

    Markus Ralser

    Insert

    SPE1
    

    Tags

    Histidine

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. p(+)MV-NSE-FlagrP-3g_haP-F497A-3p

    Plasmid

    #58799

    Purpose

    Expresses recombinant measles virus with duplicated P gene. wt P gene and P-F497A gene are tagged with Flag and HA peptides and si1 (siGFP) and si2 (siP) target sequences, respectively.

    Depositor

    Branka Horvat

    Insert

    Measles virus antigenome
    

    Tags

    Flag and Ha

    Promoter

    T7

    Available Since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV Ndc1-V5

    Plasmid

    #175115

    Purpose

    Lentiviral expression of mouse Ndc1-V5

    Depositor

    Larry Gerace

    Insert

    Ndc1 (Ndc1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. LCB7_crtYB

    Plasmid

    #140232

    Purpose

    Expression of gene coding lycopene bifunctional β-cyclase for production beta-carotene from lycopene

    Depositor

    Kang Zhou

    Insert

    phytoene-beta carotene synthase
    

    Expression

    Bacterial

    Mutation

    K307E

    Promoter

    pthrC3

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSB700-ACTC1-Puro

    Plasmid

    #128324

    Purpose

    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)

    Depositor

    Alejandro Chavez

    Insert

    gRNA against human ACTC1
    

    Expression

    Mammalian

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103 WWP2 WW domain #1

    Plasmid

    #104212

    Purpose

    Bacterial expression of WW domain #1 from WWP2

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    WWP2 WW domain #1 (WWP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    March 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHH0103 WWP2 WW domain #3

    Plasmid

    #104214

    Purpose

    Bacterial expression of WW domain #3 from WWP2

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    WWP2 WW domain #3 (WWP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pHH0103 WWP2 WW domain #2

    Plasmid

    #104213

    Purpose

    Bacterial expression of WW domain #2 from WWP2

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    WWP2 WW domain #2 (WWP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pHH0103 WWP2 WW domain #4

    Plasmid

    #104215

    Purpose

    Bacterial expression of WW domain #4 from WWP2

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    WWP2 WW domain #4 (WWP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mPA-GFP-mPlum-FRET-10

    Plasmid

    #58154

    Purpose

    Localization: Cytosolic FRET, Excitation: 504 / 590, Emission: 517 / 649

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    mPA-GFP and mPlum

    Expression

    Mammalian

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAG472 H2B-iFAST

    Plasmid

    #130818

    Purpose

    Expresses H2B-iFAST in mammalian cells (nuclear labeling)

    Depositor

    Arnaud Gautier

    Insert

    H2B-iFAST
    

    Tags

    myc-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJK402

    Plasmid

    #72415

    Purpose

    Produces Acetobacter aceti 1023 Est03, N-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    4-hydroxybenzoyl-CoA thioesterase
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLIX403-EYFP-ccdB-EBFP2

    Plasmid

    #158553

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pJK580

    Plasmid

    #71694

    Purpose

    Produces Acetobacter aceti 1023 thioredoxin with a C-terminal His6 tag (AaTrxAH6)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Paxillin_PSCFP2_ mEOS2

    Plasmid

    #114192

    Purpose

    studying focal adhesion proteins by superresolution microscopy (PALM) with dual labelling

    Depositor

    Aleksandra Radenovic

    Insert

    Paxillin (PXN Chicken)
    

    Tags

    PS-CFP2 and mEos2

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCAGGS-S-P1-FLAG

    Plasmid

    #177095

    Purpose

    P1 variant S-protein with c-terminal FLAG tag

    Depositor

    Daniel Conway

    Insert

    S-protein P1 (S SARS-CoV-2)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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30. pBS3ndd

    Plasmid

    #97354

    Purpose

    Low background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, kanamycin resistant

    Depositor

    Jamorn Somana

    Insert

    Nucleoid Disruptive Domain
    

    Use

    Cloning

    Expression

    Bacterial

    Mutation

    changed methionine 1 to isoleucine

    Promoter

    E. coli lacZ promoter

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HC080

    Plasmid

    #59545

    Purpose

    Expresses yeast Atg13 under the endogenous promoter with a GFP N-terminal tag

    Depositor

    Daniel Klionsky

    Insert

    ATG13 (ATG13 Budding Yeast)
    

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    AvrII site created after start codon

    Promoter

    ATG13

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

32. Clim-DC1-mCherry-H2B-Venus

    Plasmid

    #129559

    Purpose

    nuclear marker

    Depositor

    Inaki Ruiz-Trillo

    Insert

    mcherry and Histone 2B fused to venus
    

    Expression

    Bacterial

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 4ROJ

    Plasmid

    #64661

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    VAV2:JMC092-C08:C231327 (VAV2 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    May 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

34. U6-Lin28aIR

    Plasmid

    #120530

    Purpose

    dsRNA expression

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRK GFP Vinculin

    Plasmid

    #50531

    Depositor

    Kenneth Yamada

    Insert

    Vinculin (VCL Chicken)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

36. pORTMAGE302A

    Plasmid

    #128970

    Purpose

    Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with high temperature induction

    Depositor

    George Church

    Inserts

    PapRecT
    mutL E32K
    

    Expression

    Bacterial

    Mutation

    E32K

    Promoter

    cI-pL

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PB CAG-R-3xSV40pA-R-td-sfGFP

    Plasmid

    #177150

    Purpose

    CAG tdGFP dre/rox reporter piggyBac transposon (3x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Dre / rox ; piggybac

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Abr in pAcG2T

    Plasmid

    #38162

    Depositor

    Nora Heisterkamp

    Insert

    ABR (ABR Human)
    

    Tags

    GST

    Expression

    Insect

    Promoter

    polyhedron

    Available Since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

39. pTWEEN-miR-224

    Plasmid

    #35683

    Depositor

    George Calin

    Insert

    nsa-mir-224 (MIR224 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

40. pJW1328

    Plasmid

    #69488

    Purpose

    sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegans

    Depositor

    Jordan Ward

    Insert

    sgRNA(F+E) targeting pha-1
    

    Expression

    Worm

    Promoter

    R07E5.16 U6 promoter

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge