We narrowed to 6,649 results for: SIM;

1. pJAT43

   Plasmid

   #204299

   Purpose

   For introducing heat shock inducble B3 recombinase. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ie1

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQTEV-PPP1R1A

   Plasmid

   #31331

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

   Insert

   protein phosphatase 1 (PPP1R1AP1 Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

   Plasmid

   #205278

   Purpose

   Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1 omegaRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMJ333: pU6-MmeFz2_omegaRNA-entry

   Plasmid

   #205267

   Purpose

   Human expression vector for MmeFz2 omegaRNA (BbsI guide entry site)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   MmeFz2 omegaRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SPOON/ pcDNA3

   Plasmid

   #124840

   Purpose

   mammalian expression of SPOON, a spontaneously blinking green fluorescent protein

   Depositor

   Takeharu Nagai

   Article

   Arai et al ACS Chem Biol. 2018 Aug 17;13(8):1938-19

   Insert

   SPOON
   

   Use

   Tags

   No tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PlayBack-Ef1a-EcoR1

   Plasmid

   #203311

   Purpose

   For expression of eukaryotic genes of interest with Ef1a promoter with EcoRI as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pQTEV-PDAP1

   Plasmid

   #31586

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

   Insert

   PDGFA associated protein 1 (PDAP1 Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKHR7

   Plasmid

   #74624

   Purpose

   a cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.

   Depositor

   David Grunwald

   Article

   Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

   Insert

   cmlc2::mCherry
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   cmlc2

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAGD236

   Plasmid

   #219497

   Purpose

   pCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoter

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

   Insert

   sfGFP, Eco1 msr, Eco1 RT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAGD248

    Plasmid

    #219498

    Purpose

    Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMAL358

    Plasmid

    #169147

    Purpose

    Encodes OptoQ-AMP1

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. apt-CBE-3'end

    Plasmid

    #219939

    Purpose

    Mammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP marker

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    MCP_32-aa_evo-rA1_2xUGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. apt-ABE8e

    Plasmid

    #219937

    Purpose

    Mammalian expression of apt-ABE8e protein (Com_93aa-linker_TadA8e) with P2A-EGFP marker

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Com_93-aa_TadA8e
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQTEV-CIP29

    Plasmid

    #34822

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    cytokine induced protein 29 kDa (SARNP Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMAL513

    Plasmid

    #169153

    Purpose

    Encodes OptoQ-INVRT1

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSSM16: pCMV-HA-NLS-SpuFz1

    Plasmid

    #205271

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEN_TRE_GFP

    Plasmid

    #25767

    Purpose

    Entry vector with TRE promoter driving GFP expression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    July 14, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. 3TERM_35S

    Plasmid

    #136114

    Purpose

    3TERM after CDS/ CDS12-CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    3TERM_35S (CaMV)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pQTEV-PSMD11

    Plasmid

    #31333

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 (PSMD11 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJAT21

    Plasmid

    #204309

    Purpose

    For generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthparts

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-GFPn
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ie1

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge