We narrowed to 14,065 results for: SHI

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pGS_129

Plasmid

#160652

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

pZ estradiol inducible promoter

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-mGFAP(ABC1D)-2pabPAC(F198Y)

Plasmid

#234548

Purpose

to express a version with reduced constitutive activity (thanks to point mutation F198Y) of the optogenetic tool 2pabPAC, which increases cAMP levels when stimulated by blue light, in astrocytes

Depositor

Hajime Hirase

Article

Vittani et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

2pabPAC(F198Y)

Use

AAV

Mutation

F198Y

Promoter

mGFAP(ABC1D)

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-Myr-Flag-IKBKE

Plasmid

#15293

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKBKE (IKBKE Human)

Use

Retroviral

Tags

Myr-Flag

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins

Plasmid

#78099

Purpose

Nuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

Histone H2B - Citrine (H2BC21 Synthetic, Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

5xTetO pEF alpha

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4

Plasmid

#78349

Purpose

Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Synthetic, Human)

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pIG-747pUC19-tNGFR-P2A-CD7

Plasmid

#186086

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CD7 (CD7 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Archon2-KGC-GFP-ER2-WPRE

Plasmid

#108424

Purpose

Archon2 fluorescent voltage reporter in mammalian expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon2-KGC-EGFP-ER2

Expression

Mammalian

Promoter

CAG

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-p57

Plasmid

#101760

Purpose

p57 promoter reporter

Depositor

Yuh-Shan Jou

Article

Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

Insert

cyclin dependent kinase inhibitor 1C promoter (CDKN1C Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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PEA1-Nuclease-GFP

Plasmid

#171994

Purpose

Delivers all prime editing nuclease components in a single, GFP selectable plasmid

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Expression

Mammalian

Mutation

GC to CA point mutation in SpCas9 to restore nucl…

Promoter

CMV for Cas9, U6 for gRNAs

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBFC0625

Plasmid

#186691

Purpose

VcDART under control of Lac promoter, Vc_lacZ_α_1 gRNA, GmR+sfGFP cargo

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_lacZ_α_1 Spacer crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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