We narrowed to 15,050 results for: pET

1. pET28a-anticalin (N116amb)-His

   Plasmid

   #168045

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Asn 116 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-anticalin (N21amb)-His

   Plasmid

   #168041

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Asn 21 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b p97-His D592Amb

   Plasmid

   #169022

   Purpose

   Expresses p97-His D592Amb (incorporates noncanonical amino acid at position 592 via amber suppression) in bacteria

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p97
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   D592TAG

   Promoter

   T7

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-anticalin (I55amb)-His

   Plasmid

   #168042

   Purpose

   Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Tags

   6x Histag

   Expression

   Bacterial

   Mutation

   Ile 55 was mutated to amber codon (TAG)

   Promoter

   T7 promoter

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETCON-Aga2p-TAPBPR-myc

   Plasmid

   #153486

   Purpose

   Yeast expression of Aga2p-TAPBPR-myc

   Depositor

   Erik Procko

   Article

   McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

   Insert

   TAPBPR (TAPBPL Human)
   

   Tags

   Aga2P and c-myc epitope tag

   Expression

   Yeast

   Promoter

   GAL

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET19b-rDAD2 cPGFP(N180)

   Plasmid

   #166001

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   rDAD2 cPGFP(N180)
   

   Expression

   Bacterial

   Mutation

   LSSmOrange- V49A (please see depositor comments)

   Promoter

   T7 promoter

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET19b-DAD2 cPGFP(A149)

   Plasmid

   #165998

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   DAD2 cPGFP(A149)
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b/GVcl 1-1066 /*TBM

   Plasmid

   #162781

   Purpose

   amplification of Gallus gallus Vcl mutated in Tankyrase Binding Domain II

   Depositor

   Laura Lafon-Hughes

   Article

   et al PeerJ

   Insert

   Gallus gallus vinculin (VCL Chicken)
   

   Expression

   Bacterial

   Mutation

   changed Gly 454 to Val

   Available Since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Pet22b-mEGFP-YAP-C-IDR

   Plasmid

   #166441

   Purpose

   Bacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion protein

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP, YAP-C-IDR(266-504) (YAP1 Human)
   

   Expression

   Bacterial

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET19b-rShHTL7 cPGFP(E167)

    Plasmid

    #166004

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rShHTL7 cPGFP(E167)
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET19b-rShHTL7 cPGFP(D165)

    Plasmid

    #166003

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rShHTL7 cPGFP(D165)
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET19b-rShHTL7 cPGFP(C164)

    Plasmid

    #166002

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rShHTL7 cPGFP(C164)
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET19b-DAD2 cPGFP(N180)

    Plasmid

    #166000

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    DAD2 cPGFP(N180)
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET19b-DAD2 cPGFP(A165)

    Plasmid

    #165999

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    DAD2 cPGFP(A165)
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET19b-HisDAD2 cPGFP(G164)

    Plasmid

    #165997

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    HisDAD2 cPGFP(G164)
    

    Tags

    6His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET19b-rShHTL7 cPGFP(L166)

    Plasmid

    #165995

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rShHTL7 cPGFP(L166)
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET19b-DAD2 cPGFP(G164)

    Plasmid

    #165993

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    DAD2 cPGFP(G164)
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM10 with rat Δ192Annexin A11

    Plasmid

    #164498

    Purpose

    For bacterial expression of rat His-tagged Δ192Annexin A11

    Depositor

    Anni Vedeler

    Article

    Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

    Insert

    rat Δ192Annexin A11 (Anxa11 Rat)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    N-terminal truncation

    Promoter

    T7

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM10 with rat Δ188Annexin A11

    Plasmid

    #164497

    Purpose

    For bacterial expression of rat His-tagged Δ188Annexin A11

    Depositor

    Anni Vedeler

    Article

    Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

    Insert

    rat Δ188Annexin A11 (Anxa11 Rat)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    N-terminal truncation

    Promoter

    T7

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CPH1141 (pET151_Spt6 1247-1451)

    Plasmid

    #91815

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    deleted residues 1-1246

    Promoter

    T7

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET15-His-∆NGlpG(Y205F)

    Plasmid

    #155132

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y205F, insert codes for GlpG residues 87-276

    Promoter

    T7

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pETDUET-GST AO7RE Y165A

    Plasmid

    #139428

    Purpose

    GST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed tyrosine 165 to an alanine

    Promoter

    T7 promoter-1

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pETDUET-GST AO7RE P199A

    Plasmid

    #139427

    Purpose

    GST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed proline 199 to an alanine

    Promoter

    T7 promoter-1

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pETDUET-GST AO7RE I137A

    Plasmid

    #139319

    Purpose

    GST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed isoleucine 137 to an alanine

    Promoter

    T7 promoter-1

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-Himar-dCas9-mammalian

    Plasmid

    #138414

    Purpose

    Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar-dCas9
    

    Tags

    3xFLAG, 6xHis, and SV40 NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]

    Plasmid

    #136688

    Purpose

    Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.

    Depositor

    Colin Garvie

    Article

    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

    Insert

    Beclin-1 (BECN1 Human)
    

    Tags

    Trigger Factor

    Expression

    Bacterial

    Mutation

    DNA optimized for E. coli expression w/ three mut…

    Promoter

    T7

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-29b-3xVSVG-GSK3(1-97)

    Plasmid

    #129583

    Purpose

    Encodes VSVG tagged GSK3(1-97) for purification by nickel affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    GSK3a(1-97) (GSK3A Human)
    

    Tags

    6xHistidine

    Expression

    Bacterial

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. pJUMP18-RBS-pET-MV_RN

    Plasmid

    #127014

    Purpose

    Basic Part RN- RBS+N-terminus; B0032m Ribosome Binding Site + Met-Val N-terminus start.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Insert

    Part RBS-pET-MV_RN
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET24a-VHH-tev-2xTS

    Plasmid

    #117750

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP, tev cleavage site and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

    Insert

    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFS_0362_pET30-RT-CRISPR_Candidatus_Accumulibacter_sp._BA-91_ARRAY_1

    Plasmid

    #116974

    Purpose

    Expresses CaBA-91RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Candidatus Accumulibacter sp. BA-91 RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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