We narrowed to 10,599 results for: cat.1

1. pcDNA3-Tsapphire-StagRFP-SrcAR

   Plasmid

   #173018

   Purpose

   Long-Stokes-shift green-red FRET-based Src activity reporter for multiplexed imaging applications.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   Tsapphire-StagRFP-SrcAR
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM11-6xHis-TEV-hORF1p_K3AK4A

   Plasmid

   #202585

   Purpose

   Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   ORF1p
   

   Use

   Tags

   6x His and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Changed ORF1p Lysine 3 to Alanine, changed ORF1p …

   Promoter

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDH-hSyn-NCreIntN-T2A-mTagBFP2

   Plasmid

   #160502

   Purpose

   Expresses NCreIntN in neurons in split intein-mediated split-Cre recombinase applications

   Depositor

   Shawn Je

   Article

   Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

   Insert

   NCreIntN
   

   Use

   Cre/Lox and Lentiviral

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt

   Plasmid

   #194519

   Purpose

   Dual fluorescence reporter for studying protein degradation

   Depositor

   Tommer Ravid

   Article

   Mashahreh et al Nat Commun. 2022 Dec 8;13(1):7588. doi:

   Insert

   TIF4631 leader sequence
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-CAG-NCreIntN-T2A-mTagBFP2

   Plasmid

   #160500

   Purpose

   Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

   Depositor

   Shawn Je

   Article

   Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

   Insert

   NCreIntN
   

   Use

   Cre/Lox and Lentiviral

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pZA8

   Plasmid

   #158486

   Purpose

   Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codon

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1
   

   Use

   Golden gate entry vector

   Tags

   Expression

   Mutation

   L17E, D481V, STOP codon

   Promoter

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEE491

   Plasmid

   #149292

   Purpose

   T-DNA encoding TRV2 with tRNA multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

   Depositor

   Daniel Voytas

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   p35S:TRV2_tRNA_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pQCi-mB2m-sgRNA

   Plasmid

   #154090

   Purpose

   pQCi backbone with sgRNA targeting murine Beta-2-microglobulin

   Depositor

   Scott McComb

   Article

   Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

   Insert

   sgRNA targeting murine B2M locus
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO-puro-sh-mFAK B

   Plasmid

   #37018

   Depositor

   Bob Weinberg

   Article

   Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

   Insert

   sh-mFAK B (Ptk2 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 31, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pEE531

    Plasmid

    #149290

    Purpose

    T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CEBPalpha NGFR

    Plasmid

    #44627

    Depositor

    Ellen Rothenberg

    Article

    Del Real et al Development. 2013 Mar;140(6):1207-19. do

    Insert

    CEBPalpha (Cebpa Mouse)
    

    Use

    Retroviral

    Tags

    IRES and truncated NGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCDH-CAG-NCreIntN-EF1a-mTagBFP2

    Plasmid

    #160507

    Purpose

    Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    NCreIntN
    

    Use

    Cre/Lox and Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDH-CAG-IntCCCre-T2A-CopGFP

    Plasmid

    #160501

    Purpose

    Expresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    CopGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDH-hSyn-IntCCCre-T2A-CopGFP

    Plasmid

    #160503

    Purpose

    Expresses IntCCCre in neurons in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    CopGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    March 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDH-CAG-IntCCCre-EF1a-Cerulean

    Plasmid

    #160508

    Purpose

    Expresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP ratAPOBEC1 L173A/G227A

    Plasmid

    #167169

    Purpose

    Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.

    Depositor

    Silvestro Conticello

    Article

    Chieca et al bioRxiv 2021

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Leucine 173 to Alanine; changed Glycine 2…

    Promoter

    CMV promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEE495

    Plasmid

    #149291

    Purpose

    T-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMP650

    Plasmid

    #135000

    Purpose

    VP64-m6A Reader. Activates transcription near Gm6ATC sites. pUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoR

    Depositor

    Ahmad Khalil

    Article

    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

    Insert

    pUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    DpnI(aa146-254), V5 tag, and VP-64

    Expression

    Mammalian

    Mutation

    DpnI truncated to aa146-254

    Promoter

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-His6-OAZ-GSY-SPM-Ctag

    Plasmid

    #163613

    Purpose

    Expresses truncated human Ornithine Decarboxylase Antizyme fused to SPM in bacterial cells for calcium-induced covalent coupling to Ornithine Decarboxylase.

    Depositor

    Mark Howarth

    Article

    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

    Insert

    OAZ-GSY-SPM
    

    Use

    Tags

    C-tag and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-NIR-FbLAG30

    Plasmid

    #220748

    Purpose

    NIR-FbLAG30 expression in bacteria.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    NIR-FbLAG30
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Bacterial

    Mutation

    For bacterial cytoplasmic expression, the pelB si…

    Promoter

    T7

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pETM11-6xHis-TEV-hORF1p_StammerAAA

    Plasmid

    #202587

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. eSrtA-MT4

    Plasmid

    #200306

    Purpose

    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

    Insert

    eSrtA-LPETG-MT4 (MT4 Human)
    

    Use

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pXD70LacZ8-CadR-Pcadh-LacZ

    Plasmid

    #191623

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ with transcriptional regulator CadR

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pZA7

    Plasmid

    #158485

    Purpose

    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E with STOP codon

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1
    

    Use

    Golden gate entry vector

    Tags

    Expression

    Mutation

    L17E, STOP codon

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pZA15

    Plasmid

    #158493

    Purpose

    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E without STOP codon

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1
    

    Use

    Golden gate entry vector

    Tags

    Expression

    Mutation

    L17E, no STOP codon

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pZA6

    Plasmid

    #158484

    Purpose

    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V with STOP codon

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1
    

    Use

    Golden gate entry vector

    Tags

    Expression

    Mutation

    D481V, STOP codon

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pZA14

    Plasmid

    #158492

    Purpose

    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V without STOP codon

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1
    

    Use

    Golden gate entry vector

    Tags

    Expression

    Mutation

    D481V, no STOP codon

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIC26

    Plasmid

    #44572

    Depositor

    Iain Cheeseman, Arshad Desai

    Article

    Cheeseman et al Sci STKE. 2005 Jan 11;2005(266):pl1.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP, s peptide, and tev

    Expression

    Worm

    Mutation

    Promoter

    pie-1

    Available since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGIPz-PD-L1 4NQ

    Plasmid

    #121487

    Purpose

    Expression of human PD-L1 4NQ with PD-L1 shRNA

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Inserts

    PD-L1 4NQ
    hPD-L1 shRNA
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N35Q, N192Q, N200Q and N219Q

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Lenti ABE7.10-N-AIDmax

    Plasmid

    #157949

    Purpose

    Lentiviral expression of ABE7.10-N-AIDmax

    Depositor

    Dali Li

    Article

    Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Lenti ABE7.10-N-AIDmax
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HOXA5 (human) HIS-tag pET

    Plasmid

    #8562

    Depositor

    Corey Largman

    Insert

    HOXA5 (HOXA5 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    SacI site at amino acids 1-2 was used to clone in…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

32. pAf-CRISPR-yA

    Plasmid

    #191015

    Purpose

    CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

    Insert

    yA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Aspergillus flavus U6

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRedF

    Plasmid

    #118057

    Purpose

    GBPart - RedF Luciferase CDS

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    Red Firefly Luciferase CDS
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Red mutant S286Y

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p2xp53_RE

    Plasmid

    #118053

    Purpose

    GBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    2 copies of the p53 DNA binding motif
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. eSrtA-MT3

    Plasmid

    #200305

    Purpose

    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT3. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

    Insert

    eSrtA-LPETG-MT3 (MT3 Human)
    

    Use

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEYN12-mflon

    Plasmid

    #165581

    Purpose

    Lon protease from Mesoplasma florum

    Depositor

    Daniel Ducat

    Article

    Sakkos et al ACS Synth Biol. 2021 Jul 16;10(7):1667-1

    Insert

    lon protease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptrc

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAM2314-RbcS-mNG

    Plasmid

    #165582

    Purpose

    Cyanobacterial carboxysome reporter with mNeonGreen driven by native rbcS promoter

    Depositor

    Daniel Ducat

    Article

    Sakkos et al ACS Synth Biol. 2021 Jul 16;10(7):1667-1

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Prbcs

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TFORF0267

    Plasmid

    #142502

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF4 (TCF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TFORF0256

    Plasmid

    #142496

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF4 (TCF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TFORF0263

    Plasmid

    #142500

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF4 (TCF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge