We narrowed to 8,360 results for: construct

1. pPB-DEST-R1R2-pNeo

   Plasmid

   #141057

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Neomycin resistance gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHFX-B60-EPSUD

   Plasmid

   #174390

   Purpose

   Plasmids for gene knockout were constructed based on suicide plasmid pHFX-B60

   Depositor

   Jing Han

   Article

   Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

   Insert

   EPSUD
   

   Use

   Archaeal gene knockout vector for haloferax medit…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCFJ150-cul4

   Plasmid

   #80353

   Purpose

   Pmex-5:GFP::H2B::PEST::cul-4 3'UTR reporter construct used for microinjection

   Depositor

   Sean Ryder

   Article

   Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

   Insert

   Pmex-5:GFP::H2B::PEST::cul-4 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. C513-E07: attB5r-<p10p-polyhedrinp>-attB1r

   Plasmid

   #162949

   Purpose

   Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENTR2-L3-SfoI-EGFP-secNanoLuc-PBL-R1

   Plasmid

   #141025

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTR-L1-PGK-Neo-L2

   Plasmid

   #141007

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Neomycin resistance gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SMAD4 KO Neo

   Plasmid

   #16646

   Depositor

   Bert Vogelstein

   Article

   Zawel et al Proc Natl Acad Sci U S A. 2002 Mar 5. 99

   Insert

   Smad4 knock-out construct (SMAD4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 13, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pENTR-L3-Cas9-L2

   Plasmid

   #141038

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Gal4pBS34-Pleum

   Plasmid

   #60334

   Purpose

   Contains a promoter of two gal binding sites coupled wiht a core promoter derived from native Leucine promoter.

   Depositor

   Hal Alper

   Article

   Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

   Insert

   promoter
   

   Use

   Synthetic Biology; Yeast synthetic hybrid promote…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 11, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. hRad51(G151D)–Cas9(D10A)

    Plasmid

    #125565

    Purpose

    Expresses the Rad51(G151D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51(G151D)–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSO7

    Plasmid

    #106365

    Purpose

    To insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the epidermis.

    Depositor

    Jonathan Ewbank

    Article

    Lee et al Virulence. 2018 Dec 31;9(1):648-658. doi

    Insert

    ttTi4348_ SEC _pcol-19_OspF_3'unc54UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Gal4pBS12-Pleum

    Plasmid

    #60326

    Purpose

    Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 11, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. ReaLiSM A1-Hinge-GPIba+2cys

    Plasmid

    #59581

    Purpose

    express VWF A1-GPIba ReaLiSM construct in mammalian cells

    Depositor

    Timothy Springer

    Article

    Kim et al Nature. 2010 Aug 19;466(7309):992-5. doi

    Insert

    VWF A1-linker-GPIba (VWF Human)
    

    Use

    Tags

    6His

    Expression

    Mammalian

    Mutation

    Promoter

    chick beta-actin

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

14. UASGAL-Pleum

    Plasmid

    #60333

    Purpose

    Contains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. hRad51(F86E)–Cas9(D10A)

    Plasmid

    #125564

    Purpose

    Expresses the Rad51(F86E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51(F86E)–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEMT-PT2A-Tdtomato-GFP-iRFP670

    Plasmid

    #111814

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    Tdtomato
    GFP
    iRFP670
    

    Use

    Cloning intermediate

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EcPhnD90.L1∆∆AD.L297RL301R.pRSET

    Plasmid

    #33376

    Purpose

    a single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFP

    Depositor

    Loren Looger

    Article

    Alicea et al J Mol Biol. 2011 Oct 12.

    Insert

    phnD based Phosphonate Biosensor (phnD E. coli)
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    cpGFP146 variant from GCaMP2 inserted in place of…

    Promoter

    T7

    Available since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Gal4pBS24-Pleum

    Plasmid

    #60327

    Purpose

    Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. rAAV-Ef1α-TdTomato-WPRE-polyA

    Plasmid

    #241828

    Purpose

    AAV construct for constitutive expression of TdTomato.

    Depositor

    Benjamin Arenkiel

    Article

    Belfort et al Front Neurosci. 2025 Feb 13;19:1531122.

    Insert

    TdTomato-WPRE-polyA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV_CK8e_Cas9_miniPA

    Plasmid

    #226141

    Purpose

    AAV construct for HITI insert with CK8e promoter and SpCas9

    Depositor

    Christopher Nelson

    Article

    Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

    Insert

    Cas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CK8e

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pME_G_0_1-2_004_OC5'4

    Plasmid

    #235804

    Purpose

    5' Operon Connector 4 in the M1 position used to construct operons

    Depositor

    Tobias Erb

    Insert

    5' Operon Connector 4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. POC1550

    Plasmid

    #226497

    Purpose

    pMOBC360_VM_C1 assembled plasmid (Chloramphenicol R) containing four inserts (POC1546-POC1549) assembled in POC1525 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. POC1546

    Plasmid

    #226487

    Purpose

    pMOBK360_VL_N1 assembled plasmid (Kanamycin R) containing four inserts (POC1535-POC1538) assembled in POC1518 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    ssembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. POC1547

    Plasmid

    #226489

    Purpose

    pMOBK360_VM_N2 assembled plasmid (Kanamycin R) containing three inserts (POC1539-POC1541) assembled in POC1519 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. POC1548

    Plasmid

    #226490

    Purpose

    pMOBK360_VM_N3 assembled plasmid (Kanamycin R) containing two inserts (POC1542-POC1543) assembled in POC1519 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. POC1549

    Plasmid

    #226491

    Purpose

    pMOBK360_VR_N4 assembled plasmid (Kanamycin R) containing two inserts (POC1544-POC1545) assembled in POC1520 using the site-selective methylation protection approach

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

    Insert

    Assembled construct flanked by methylation-protectable BsaI sites and containing several always-methylable internal BsaI sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPB-CAG-KRAB-dCas9-P2A-mCherry-pgk-hph

    Plasmid

    #233067

    Purpose

    Piggybac CRISPRi construct

    Depositor

    Markus Landthaler

    Article

    Vučićević et al bioRxiv 2024.09.12.612198

    Insert

    KRAB-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pME_G_0_5-6_010_OC3'5

    Plasmid

    #235966

    Purpose

    3' Operon Connector 5 in the M2 position used to construct operons

    Depositor

    Tobias Erb

    Insert

    OC3'5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pME_G_0_5-6_011_OC3'6

    Plasmid

    #235967

    Purpose

    3' Operon Connector 6 in the M2 position used to construct operons

    Depositor

    Tobias Erb

    Insert

    OC3'6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pME_G_0_5-6_012_OC3'7

    Plasmid

    #235968

    Purpose

    3' Operon Connector 7 in the M2 position used to construct operons

    Depositor

    Tobias Erb

    Insert

    OC3'7
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pME_G_0_5-6_006_OC3'1

    Plasmid

    #235962

    Purpose

    3' Operon Connector 1 in the M2 position used to construct operons

    Depositor

    Tobias Erb

    Insert

    OC3'1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pME_G_0_5-6_007_OC3'2

    Plasmid

    #235963

    Purpose

    3' Operon Connector 2 in the M2 position used to construct operons

    Depositor

    Tobias Erb

    Insert

    OC3'2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pME_G_0_5-6_008_OC3'3

    Plasmid

    #235964

    Purpose

    3' Operon Connector 3 in the M2 position used to construct operons

    Depositor

    Tobias Erb

    Insert

    OC3'3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pME_G_0_5-6_009_OC3'4

    Plasmid

    #235965

    Purpose

    3' Operon Connector 4 in the M2 position used to construct operons

    Depositor

    Tobias Erb

    Insert

    OC3'4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pME_G_0_1-2_007_OC5'7

    Plasmid

    #235807

    Purpose

    5' Operon Connector 7 in the M1 position used to construct operons

    Depositor

    Tobias Erb

    Insert

    5' Operon Connector 7
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pME_G_0_1-2_002_OC5'2

    Plasmid

    #235802

    Purpose

    5' Operon Connector 2 in the M1 position used to construct operons

    Depositor

    Tobias Erb

    Insert

    5' Operon Connector 2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pME_G_0_1-2_003_OC5'3

    Plasmid

    #235803

    Purpose

    5' Operon Connector 3 in the M1 position used to construct operons

    Depositor

    Tobias Erb

    Insert

    5' Operon Connector 3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pME_G_0_1-2_005_OC5'5

    Plasmid

    #235805

    Purpose

    5' Operon Connector 5 in the M1 position used to construct operons

    Depositor

    Tobias Erb

    Insert

    5' Operon Connector 5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pME_G_0_1-2_006_OC5'6

    Plasmid

    #235806

    Purpose

    5' Operon Connector 6 in the M1 position used to construct operons

    Depositor

    Tobias Erb

    Insert

    5' Operon Connector 6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pME_G_0_1-2_001_OC5'1

    Plasmid

    #235801

    Purpose

    5' Operon Connector 1 in the M1 position used to construct operons

    Depositor

    Tobias Erb

    Insert

    5' Operon Connector 1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET28-NFLtail

    Plasmid

    #232055

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFL tail domain

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Light tail domain
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    cDNA codon-optimized for E. coli; added N-termina…

    Promoter

    T7

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET28-NFMshuf1

    Plasmid

    #232058

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 1

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Medium tail domain, charge shuffle 1
    

    Use

    Tags

    6xHis, thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET28-NFHtail

    Plasmid

    #232057

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFH tail domain

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Heavy tail domain
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    added N-terminal tryptophan and tyrosine, and cys…

    Promoter

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET28-NFMshuf2

    Plasmid

    #232059

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 2

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Medium tail domain, charge shuffle 2
    

    Use

    Tags

    6xHis, thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. ECMAB - pET22b Ag43 160N 6H Mms6

    Plasmid

    #225539

    Purpose

    Construction of extracellular magnetite accumulating bacterial systems.

    Depositor

    Urartu Seker

    Article

    Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

    Inserts

    Iron binding protein
    Autotransporter protein
    

    Use

    Tags

    no

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. CL7_2xNLS_iGeoCas9(C2)_2xNLS_His

    Plasmid

    #222988

    Purpose

    Bacterial expression of iGeoCas9(C2) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(C2)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. pTol1 - 14xUAS:CaSR-EGFP

    Plasmid

    #191502

    Purpose

    A UAS construct used to create transgenic fish that express CaSR-EGFP when crossed to a fish carrying a Gal4.

    Depositor

    Michael Granato

    Article

    Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

    Insert

    CaSR-EGFP-SV40
    

    Use

    Creation of transgenic fish using tol1 transgenes…

    Tags

    Expression

    Mutation

    Promoter

    14X UAS

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pAAV_S34F_U2AF1

    Plasmid

    #176592

    Purpose

    Adenoviral construct to change S to F at 34th amino acid (S34F) in human U2AF1

    Depositor

    Manoj Pillai

    Article

    Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

    Insert

    U2AF1 (U2AF1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pLPP4

    Plasmid

    #209966

    Purpose

    Contains Level 0 Part: constitutive Promoter (P40) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (P40)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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50. pLP-EV

    Plasmid

    #209973

    Purpose

    Contains Level 0 Part: mock coding sequence (EV) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    CDS (mock)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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