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pCDNA4.TO-K1-2xCSTREP

Plasmid

#136148

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K1

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ER-rsTagRFP

Plasmid

#87620

Purpose

Endoplasmic reticulum (ER)-targeted rsTagRFP.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

ER-rsTagRFP

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF10_P2A_Hygro_Barcode

Plasmid

#120496

Purpose

Barcoded lentiviral vector to express KLF10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF10 (KLF10 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pH2B-mPlum/Gag

Plasmid

#102255

Purpose

co-expression of Gag and H2B Plum in mammalian cells

Depositor

Suliana Manley

Article

Gunzenhauser et al PLoS One. 2014 Dec 10;9(12):e115095. doi

Inserts

Gag
H2B Plum

Expression

Mammalian

Available Since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[closed]

Plasmid

#71810

Purpose

Generating transgenic flies for FLP-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

min.hsp70P-FRT-STOP#1-FRT-Dam-Myc[closed]

Expression

Insect

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pETM33_CLTC_Clathrin propeller

Plasmid

#178471

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CLTC_Clathrin propeller (CLTC Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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acta1-Cavin4b-Clover

Plasmid

#163854

Purpose

Zebrafish muscle specific expression of Cavin4b-Clover. Parton lab clone DQZ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Cavin4b (cavin4b Zebrafish)

Use

Zebrafish expression

Tags

Clover

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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CMV::TVA/tRFP

Plasmid

#164486

Purpose

Dual CMV promoter plasmid to express TVA and TurboRFP

Depositor

Hollis Cline

Article

Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

Inserts

TVA (CD320 Chicken)
TurboRFP

Expression

Mammalian

Promoter

sCMV and sCMV IE94

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP15

Plasmid

#158366

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP15 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Nov. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 Orf7b

Plasmid

#158376

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf7b (ORF7b )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP7

Plasmid

#158358

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP7 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pKK-FLAG-TEV

Plasmid

#105768

Purpose

Expression of your protein of interest in fusion with FLAG at the N-terminus. The tag is cleavable by TEV protease or enterokinase.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

FLAG-TEV

Expression

Mammalian

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pKK-TEV-mCherry

Plasmid

#105796

Purpose

Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-mCherry

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG/hArf4(T31N)-HA

Plasmid

#79405

Purpose

Expresses C-terminally HA-tagged Arf4(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

Insert

Arf4 (ARF4 Human)

Tags

HA

Expression

Mammalian

Mutation

T31N

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
pCAG/hArf4(WT)-mCherry

Plasmid

#79406

Purpose

Expresses C-terminally mCherry-tagged Arf4(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

Insert

Arf4 (ARF4 Human)

Tags

mCherry

Expression

Mammalian

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
pAAV.hSynap.(mem).iGlucoSnFR2.mRuby3

Plasmid

#244097

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

IgG kappa leader and mRuby3

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Halo-NEMO

Plasmid

#187899

Purpose

Mammalian cell expression of Halo-tagged NEMO

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

NEMO (Ikbkg Mouse)

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-2-SV40 polyA

Plasmid

#190866

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode2

Use

AAV

Mutation

WT

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19/mChe-Golgi

Plasmid

#183169

Purpose

Construct for transient expression of Golgi apparatus-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

mCherry-Golgi

Tags

AtRER1B, A. thaliana endoplasmic reticulum retrie…

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eGFP-SynaptoZip

Plasmid

#122525

Purpose

AAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoter

Depositor

Antonio Malgaroli

Article

Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

Insert

eGFP-SynaptoZip (Vamp2 Rat)

Use

AAV

Tags

Myc and eGFP

Expression

Mammalian

Promoter

hSyn

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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