We narrowed to 6,263 results for: wus

1. pcDNA3-FlipCherry-T2A-GFP

   Plasmid

   #124436

   Purpose

   Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipCherry-T2A-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. LcV2-Hygro

   Plasmid

   #91977

   Purpose

   LentiCRISPR v2 with Hygro cloned in place of Puro

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag

   Plasmid

   #132398

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Promoter

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 FlipGFP(Mpro) T2A mCherry

   Plasmid

   #163078

   Purpose

   Expresses FlipGFP-based activity reporter of SARS-CoV-2 main protease Mpro and also mCherry

   Depositor

   Xiaokun Shu

   Article

   Li et al PLoS Pathog. 2021 Sep 3;17(9):e1009898.

   Insert

   FlipGFP-based Mpro activity reporter and mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

   Plasmid

   #132400

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPguCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H151A; H1121A

   Promoter

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pST1374-NLS-flag-linker-Cas9

   Plasmid

   #44758

   Depositor

   Xingxu Huang

   Article

   Shen et al Cell Res. 2013 Apr 2. doi: 10.1038/cr.20

   Insert

   Human codon optimized Cas9
   

   Use

   CRISPR

   Tags

   32 amino acid linker, Flag, and NLS

   Expression

   Mammalian

   Mutation

   codon-optimized

   Promoter

   CMV

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiV_Cas9_puro

   Plasmid

   #108100

   Purpose

   Lentiviral expression plasmid of spCas9 with puromycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS promoter

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB-mTSPAN4-mCherry

   Plasmid

   #210330

   Purpose

   Overexpression of mTSPAN4

   Depositor

   Li Yu

   Article

   Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

   Insert

   mTSPAN4 (Tspan4 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15TV-L-AldA WT

   Plasmid

   #187976

   Purpose

   Expresses human aldolase A

   Depositor

   Audrey Lamb

   Article

   Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

   Insert

   AldA WT (ALDOA Human)
   

   Use

   Tags

   Hexahistag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiV_Neo_SIK3

    Plasmid

    #108103

    Purpose

    Lentiviral expression plasmid of human SIK3 cDNA with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    SIK3 (SIK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-HA-mSTING

    Plasmid

    #214157

    Purpose

    Transient expression of STING

    Depositor

    Nan Yan

    Article

    Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

    Insert

    STING (Sting1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. MTK3_038

    Plasmid

    #123752

    Purpose

    Encodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    R-FlincA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag

    Plasmid

    #132409

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dRanCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H147A; H1044A

    Promoter

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-N1 human cofilin WT

    Plasmid

    #50859

    Purpose

    expresses human cofilin fused to EGFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 (CFL1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS-FLAG-CaMKIIA

    Plasmid

    #186825

    Purpose

    Expresses FLAG-CaMKIIA in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    Camk2a (Camk2a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-f:PGC1

    Plasmid

    #1026

    Depositor

    Bruce Spiegelman

    Article

    Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

    Insert

    PPAR gamma coactivator 1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti_MS2_P65_HSF1_2A_puro

    Plasmid

    #118699

    Purpose

    To express MS2-P65-HSF1

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT3-EF1aH

    Plasmid

    #180149

    Purpose

    This plasmid is an empty vector used as the control for other plasmids which are in pT3-EF1aH backbone. It does not have any restriction site for cloning.

    Depositor

    Xin Chen

    Article

    Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a_Pro-IL-33

    Plasmid

    #214342

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL33 (IL33 Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a_Pro-IL-1β

    Plasmid

    #214340

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL1B (IL1B Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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