We narrowed to 13,796 results for: CAN
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Plasmid#105934PurposeMammalian expression of myc-his tagged AXL kinase dead (K558R) mutantDepositorInsertAXL (AXL Human)
TagsHis and mycExpressionMammalianMutationK558R, kinase deadPromotertet-inducible CMVAvailable SinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7b-5p
Plasmid#103148PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7b-5p target (MIRLET7B Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-19a-3p
Plasmid#103323PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-19a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-19a-3p target (MIR19A Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
MultiMate 5 colors
Plasmid#206266PurposeExpression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)DepositorInsertH2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)
UseRecombinant baculovirus production, cre acceptor…ExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
SNAP-ACE2
Plasmid#178592PurposeExpresses SNAP-fused ACE2 protein that can be fluorescently labelled using SNAP substrates.DepositorInsertangiotensin-converting enzyme 2 (ACE2 Human)
TagsFlag and SNAPExpressionMammalianPromoterCMVAvailable SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-548e-5p
Plasmid#103634PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-548e-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-548e-5p target (MIR548E Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-PKHD1-myc
Plasmid#108407PurposeCan be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.DepositorAvailable SinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA
Plasmid#82702PurposeAAV backbone with a minimal H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.DepositorInsertH1/TO Empty gRNA Expression Cassette
UseAAV and CRISPRTagsCMV promoter driving expression of TetR-P2A-GFP-K…ExpressionMammalianAvailable SinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
p221z-erVenusYFP
Plasmid#71265PurposeEntry clone containing ER-localized Venus. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertVenusYFP
UseGatewayTagsER retention signal HDEL and signal peptide (ER)…Available SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTagGFP2-N SIN1 deltaNCD
Plasmid#124920PurposeFor expression of N-terminal Conserved Domain deletion mutant of SIN1-GFP (delta2-137)DepositorAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
p221z-erTagRFP
Plasmid#71266PurposeEntry clone containing ER-localized TagRFP. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertTagRFP
UseGatewayTagsER retention signal HDEL and signal peptide (ER)…Available SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTagGFP2-N SIN1 NCD
Plasmid#124927PurposeFor expression of NCD (a.a. 1-139) of SIN1-GFPDepositorInsertMAPKAP1 (MAPKAP1 Human)
TagsTagGFP2ExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…Available SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBZCas13b-HEPN
Plasmid#89901PurposeBacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.DepositorInsertCas13b
ExpressionBacterialMutationR116A/H121A/R1177A/H1182APromoterLacAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLXSN-Axl DN
Plasmid#65223PurposeExpression of dominant negative Axl in mammalian cellsDepositorInsertAXL DN (AXL Human)
UseRetroviralExpressionMammalianMutationdeleted cytoplasmic domainPromoterLTRAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-132-3p
Plasmid#103219PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-132-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-132-3p target (MIR132 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-EcYtR(NGS-6)-mCherry
Plasmid#217363PurposeExpresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertE. coli tyrosine tRNA mutant, "NGS-6"
UseAAVExpressionMammalianPromoterU6Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-PytR(PyOtR)-mCherry
Plasmid#204870PurposeExpresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertM. mazei pyrrolysyl tRNA mutant "PyOtR"
UseAAVExpressionMammalianMutationU25C; A3G, A4C, A5G, C6G, G63C, T65G, T66CPromoterU6Available SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
p221k-erTq2CFP
Plasmid#71264PurposeEntry clone containing ER-localized Turqoise2. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system.DepositorInsertTq2CFP
UseGatewayTagsER retention signal HDEL and signal peptide (ER)…Available SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-GFP-PKHD1
Plasmid#108408PurposeCan be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.DepositorAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-27a-3p
Plasmid#103381PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-27a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-27a-3p target (MIR27A Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only