Addgene

We narrowed to 13,298 results for: CAN

Showing: 2361 - 2380 of 13298 results

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sgMAPKAP1_2

Plasmid

#124911

Purpose

Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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sgMAPKAP1_3

Plasmid

#124913

Purpose

Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter

Plasmid

#121813

Purpose

pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-183-5p

Plasmid

#103285

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-183-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-183-5p target (MIR183 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-193a-3p

Plasmid

#103305

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-193a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-193a-3p target (MIR193A Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa VP64 Neurog2

Plasmid

#99695

Purpose

Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAV

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Mouse, Synthetic)

Use

AAV and CRISPR

Tags

VP64

Expression

Mutation

dead Cas9

Promoter

Available since

Sept. 22, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-302a-3p

Plasmid

#103400

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-302a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-302a-3p target (MIR302A Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-483-3p

Plasmid

#103550

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-483-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-483-3p target (MIR483 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-885-3p

Plasmid

#103744

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-885-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-885-3p target (MIR885 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-579-5p

Plasmid

#103661

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-579-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-579-5p target (MIR579 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-510-5p

Plasmid

#103593

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-510-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-510-5p target (MIR510 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-122a-5p

Plasmid

#103183

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-122a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-122a-5p target (MIR122 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-126-5p

Plasmid

#103193

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-126-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-126-5p target (MIR126 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIBN-TetR-tagRFP-T

Plasmid

#103809

Purpose

'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-TetR-tagRFP-T (CIB1, tetR Mustard Weed, E. coli)

Use

Tags

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-495-3p

Plasmid

#103568

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-495-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-495-3p target (MIR495 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-26a-1-3p

Plasmid

#103377

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-26a-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-26a-1-3p target (MIR26A1 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-26a-2-3p

Plasmid

#103378

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-26a-2-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-26a-2-3p target (MIR26A2 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

Plasmid

#121819

Purpose

pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-miR-31-3p

Plasmid

#103420

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-31-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-31-3p target (MIR31 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LSB-hsa-let-7f-1-3p

Plasmid

#103154

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-let-7f-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-let-7f-1-3p target (MIRLET7F1 Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2361 - 2380 of 13298 results

Results per page:

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