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Showing: 5901 - 5950 of 7988 results

  2. BAP1-C91A (siRNA resistant)

    Plasmid
    #108438
    Purpose
    Stable expression of BAP1-C91A (catalytically inactive mutant) in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.
    Depositor
    Emanuela Felley-Bosco, Rolf Stahel
    Article
    Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-
    Insert
    BAP1 (BAP1 Human)
    Expression
    Mammalian
    Mutation
    C91A: c.271T>G, c.272G>C; others: c.1641C&g…
    Promoter
    CMV
    Available Since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    108438-plasmid-map-sequence-id-209210

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  3. YFP(1-158)-beta-1 in pcDNAI/Amp

    Plasmid
    #54468
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Insert
    YFP (1-158)/beta-1 (GNB1 Human, Aequorea victoria)
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    GBeta-1 was amplified via PCR, which removed an i…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    54468-plasmid-map-sequence-id-112586

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  4. CFP(159-238)-beta-1 in pcDNAI/Amp

    Plasmid
    #55592
    Purpose
    A carboxyl-terminal CFP fragment was fused to Gbeta-1. When co-expressed with an amino-trerminal CFP or YFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    CFP (159-238)-Beta 1 (GNB1 Human, Aequorea victoria)
    Tags
    CFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    CFP(159-238) includes a substitution of His for A…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55592-plasmid-map-sequence-id-114863

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  6. GCN5 FL-dCas9-p300 core

    Plasmid
    #179554
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179554-plasmid-map-sequence-id-354754

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  20. JGI_018119

    Plasmid
    #158929
    Purpose
    Hydrocarbon Structures for Fuels and Chemicals
    Depositor
    Joint Genome Institute
    Insert
    WP_104244666_1
    Use
    Synthetic Biology
    Available Since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    158929-plasmid-map-sequence-id-316874

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  21. T4 Lysozyme N40-[ES] WT*

    Plasmid
    #18300
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Expression
    Bacterial
    Mutation
    N40-[ES] WT*
    Available Since
    Aug. 26, 2008
    Availability
    Academic Institutions and Nonprofits only

  22. mEmerald-VSVG-16

    Plasmid
    #54307
    Purpose
    Localization: Golgi/Endoplasmic Reticulum, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    VSVG
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  23. XE141 sFRP-1-CS2+

    Plasmid
    #16693
    Depositor
    Randall Moon
    Insert
    sFRP-1 (Sfrp1 Mouse)
    Use
    Xenopus expression
    Available Since
    May 22, 2009
    Availability
    Academic Institutions and Nonprofits only

  24. tdTurboRFP-VSVG-16

    Plasmid
    #58066
    Purpose
    Localization: Golgi/ER Transport, Excitation: 553, Emission: 576
    Depositor
    Michael Davidson
    Insert
    VSVG
    Tags
    tdTurboRFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. sfGFP-VSVG-16

    Plasmid
    #56490
    Purpose
    Localization: Golgi/ER Transport, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Insert
    VSVG
    Tags
    sfGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 16, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. mCerulean-VSVG-16

    Plasmid
    #55397
    Purpose
    Localization: Golgi/ER Transport, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    VSVG
    Tags
    mCerulean
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  27. makeTCR_Hs.TRAV14/DV4

    Plasmid
    #233957
    Purpose
    Hs TRAV gene for makeTCR assembly
    Depositor
    Michael Platten
    Insert
    TRAV14/DV4 (TRAV14DV4 Human)
    Use
    Synthetic Biology
    Promoter
    n/a
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233957-plasmid-map-sequence-id-468283

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  28. pJCHph

    Plasmid
    #185863
    Purpose
    The CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable marker
    Depositor
    Claudia Vickers
    Insert
    PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; HphMX6
    Expression
    Yeast
    Mutation
    None
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185863-plasmid-map-sequence-id-370806

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  29. pJCKan

    Plasmid
    #185862
    Purpose
    The CRISPR/Cas9-and-gRNA-expressing plasmid with a G418-resistent selectable marker
    Depositor
    Claudia Vickers
    Insert
    PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; KanMX4
    Expression
    Yeast
    Mutation
    None
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185862-plasmid-map-sequence-id-370803

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  30. mApple-MAP4-C-10

    Plasmid
    #54923
    Purpose
    Localization: Microtubules, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    MAP4
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    aa 640-1045 of MAP4
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  31. pHMR1B

    Plasmid
    #102958
    Purpose
    gene cloning of MR1B
    Depositor
    Gennaro De Libero, Lucia Mori
    Insert
    MR1B cDNA (MR1P1 Human)
    Use
    Cloning
    Mutation
    Stop codon has been removed by HindIII site
    Available Since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    102958-plasmid-map-sequence-id-198778

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  32. mPA-GFP-VSVG-12

    Plasmid
    #57163
    Purpose
    Localization: Golgi/ER Transport, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    VSVG
    Tags
    mPA-GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. R777-E325 Hs.SPRED3

    Plasmid
    #70609
    Purpose
    Gateway ORF clone of human SPRED3 [NM_001042522.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SPRED3 (SPRED3 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70609-plasmid-map-sequence-id-126058

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  34. mCherry-MAP4-N-10

    Plasmid
    #55076
    Purpose
    Localization: Microtubules, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    MAP4
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    aa 640-1045 of MAP4
    Promoter
    CMV
    Available Since
    Nov. 19, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. pcDNA3.1-MS2-HA-HsNeuroguidin_AA

    Plasmid
    #148237
    Purpose
    Mammalian Expression of HsNeuroguidin
    Depositor
    Elisa Izaurralde
    Insert
    HsNeuroguidin (NGDN Human)
    Expression
    Mammalian
    Mutation
    two silent mutations compared to the sequence giv…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148237-plasmid-map-sequence-id-429663

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  36. R777-E326 Hs.SPRED3-nostop

    Plasmid
    #70610
    Purpose
    Gateway ORF clone of human SPRED3 [NM_001042522.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SPRED3 (SPRED3 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70610-plasmid-map-sequence-id-126059

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  37. PLEXIN A2 (m1)

    Plasmid
    #62353
    Purpose
    Riboprobe for in vitro transcription of Plexin A2 (m1)
    Depositor
    Christiana Ruhrberg
    Insert
    Plexin A2 (Plxna2 Mouse)
    Expression
    Bacterial
    Mutation
    bp 2430-3064 of Genbank ref NM_008882.2
    Available Since
    Feb. 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  38. PLEXIN A2 (m2)

    Plasmid
    #62354
    Purpose
    Riboprobe for in vitro transcription of Plexin A2 (m2)
    Depositor
    Christiana Ruhrberg
    Insert
    Plexin A2 (Plxna2 Mouse)
    Expression
    Bacterial
    Mutation
    bp 3471-4075 of Genbank ref NM_008882.2
    Available Since
    Feb. 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  39. pCGN-Sos Cat

    Plasmid
    #32917
    Depositor
    Dafna Bar-Sagi
    Insert
    SOS1 (SOS1 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Contains only aa 564-1049
    Promoter
    CMV
    Available Since
    Jan. 31, 2012
    Availability
    Academic Institutions and Nonprofits only

  40. mEmerald-TMEM216-N-18

    Plasmid
    #54280
    Purpose
    Localization: Cilium, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    TMEM216 (Tmem216 Mouse)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  41. pAIP4_1YW5

    Plasmid
    #234235
    Purpose
    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1YW5 (ESS1 Candida albicans)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234235-plasmid-map-sequence-id-464199

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  43. KP.3

    Plasmid
    #233340
    Purpose
    expressing SARS-CoV-2 KP.3 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (KP.3) (S SARS-CoV-2)
    Expression
    Mammalian
    Mutation
    F456L, Q493E, V1104L on the basis of JN.1
    Promoter
    CMV
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    233340-plasmid-map-sequence-id-472420

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  44. pLV Tmx1-V5

    Plasmid
    #175138
    Purpose
    Lentiviral expression of V5-tagged mouse Tmx1
    Depositor
    Larry Gerace
    Insert
    Tmx1 (Tmx1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175138-plasmid-map-sequence-id-352052

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  45. pLV Ndc1-V5

    Plasmid
    #175115
    Purpose
    Lentiviral expression of mouse Ndc1-V5
    Depositor
    Larry Gerace
    Insert
    Ndc1 (Ndc1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175115-plasmid-map-sequence-id-351773

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  46. LOC201475

    Plasmid
    #25512
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    LOC201475
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  47. Protein2PAM_NmeN4G.2

    Plasmid
    #250225
    Purpose
    Express Protein2PAM_NmeN4G.2 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4G.2
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 Y960H/V979I/V980I/Q981A/E1008K/M1016R/N1…
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250225-plasmid-map-sequence-id-501785

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  48. Protein2PAM_NmeN4G.4

    Plasmid
    #250227
    Purpose
    Express Protein2PAM_NmeN4G.4 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN4G.4
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 D957G/V979I/Q981A/L990V/I1010T/G1026S/N1…
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250227-plasmid-map-sequence-id-502221

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  49. pPMS-986

    Plasmid
    #232279
    Purpose
    Protein Expression
    Depositor
    Jeremy Nathans
    Insert
    Antrx1 (Antxr1 Mouse)
    Expression
    Mammalian
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232279-plasmid-map-sequence-id-462099

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  50. pAc5.1B-lambdaN-HA-DmRRP6_AA

    Plasmid
    #148220
    Purpose
    Insect Expression of DmRRP6
    Depositor
    Elisa Izaurralde
    Insert
    DmRRP6 (Rrp6 Fly)
    Expression
    Insect
    Mutation
    two non silent muations S645 to P and A752 to V c…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148220-plasmid-map-sequence-id-290668

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Showing: 5901 - 5950 of 7988 results