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C-term_aMFx4 (4a)

Plasmid

#238902

Purpose

Plasmid insert contains the aMFx4 C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

C-term_aMFx4

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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twinStrep_FLAG_aMFx1 (4a)

Plasmid

#238904

Purpose

Plasmid insert contains the aMFx1, FLAG and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

twinStrep_FLAG_aMFx1

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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twinStrep_HA_aMFx1 (4a)

Plasmid

#238905

Purpose

Plasmid insert contains the aMFx1, HA and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

twinStrep_HA_aMFx1

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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twinStrep_aMFx1 (4a)

Plasmid

#238906

Purpose

Plasmid insert contains the aMFx1 and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

twinStrep_aMFx1

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-RYBP NZF (20-51)

Plasmid

#235652

Purpose

Bacterial expression of human RYBP NZF (20-51)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

RYBP NZF (RYBP Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 20-51 only

Promoter

T7

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-giantin-ATG_donor

Plasmid

#222316

Purpose

Perform knock-in by homologous recombination in giantin/GOLGB1 gene.

Depositor

Franck Perez

Article

Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

Insert

EGFP and homology arms for giantin/GOLGB1

Use

CRISPR

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2

Plasmid

#228829

Purpose

Insect cell expression of BICD2

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Tags

Strep

Expression

Insect

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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C1C2CMUNC2C

Plasmid

#218674

Purpose

Insect cell expression of a rat MUNC13-1 fragment containing domains C1C2BMUNC2C

Depositor

Axel Brunger

Article

Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

Insert

MUNC13 (Unc13a Rat)

Tags

GST

Expression

Insect

Mutation

Amino acids1407-1453 deleted

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-3C-hTspan12LEL(118-218)_pAcGP67a

Plasmid

#216383

Purpose

Baculovirus transfer vector to secrete human Tspan12 LEL (residues 118-218) fused to MBP

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Tetraspanin-12 (TSPAN12 Human)

Use

Baculovirus

Tags

GP64 signal sequence-MBP-3C

Expression

Insect

Promoter

Polyhedrin

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEPCTΩSP0021

Plasmid

#187561

Purpose

Module for copper-inducible expression of firefly luciferase and nanoluc luciferase driven by minimal synthetic promoters with binding sites for CUP2

Depositor

Nicola Patron

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Firefly Luciferase

Expression

Plant

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEPCTΩSP0022

Plasmid

#187562

Purpose

Module for copper-inducible expression of nanoluc luciferase and firefly luciferase driven by minimal synthetic promoters with binding sites for CUP2

Depositor

Nicola Patron

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Firefly Luciferase and NanoLuciferase

Expression

Plant

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pFBMeCP2G.10 (pc1577)

Plasmid

#211728

Purpose

Expresses MeCP2 (aa1-76) GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MeCP2 aa1-76

Tags

GFP

Expression

Insect

Promoter

Polyhedrin promotor

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-MBD2.6G (pc2396)

Plasmid

#211724

Purpose

Expresses MBD2 (aa153-235) GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.6 aa153-235

Tags

GFP

Expression

Insect

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-MeCP2Y.3 (pc1575)

Plasmid

#211717

Purpose

Expresses MeCP2 (aa1-163) YFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MeCP2 aa1-163

Tags

YFP

Expression

Insect

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-MBD2mR (pc2519)

Plasmid

#211716

Purpose

Expresses fl. MBD2 RFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2

Tags

RFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFBMBD4-C-GFP (pc1567)

Plasmid

#211714

Purpose

Expresses fl. MBD4 GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD4

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFBMBD3-C-GFP (pc1566)

Plasmid

#211713

Purpose

Expresses fl. MBD3 GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD3

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFBMBD1a-C-GFP (pc1564)

Plasmid

#211711

Purpose

Expresses fl. MBD1 GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD1

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDV02

Plasmid

#212156

Purpose

Plasmid expressing secondary alcohol dehydrogenase under constitutive promoter PgyrA control.

Depositor

Kang Zhou

Article

Tan et al ACS Synth Biol. 2023 Jul 21;12(7):2041-2

Insert

secondary alcohol dehydrogenase

Expression

Bacterial

Promoter

PgyrA

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-pOTC-GFP

Plasmid

#205724

Purpose

Expression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

pOTC-GFP

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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