We narrowed to 2,369 results for: clu

1. KRD22_HBA1(HBA1reg-HBB-longHAs)

   Plasmid

   #232403

   Purpose

   AAV production plasmid for HBA1 long HAs vector from Figs. 3-6 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank full HBA1-2A-YFP cassette. HAs are ~900bp

   Depositor

   Kyle Cromer, Matthew Porteus

   Article

   Cromer et al Nat Med. 2021 Apr;27(4):677-687. doi: 10

   Inserts

   Hemoglobin Subunit Alpha 1 (HBA1 Human)
   Human Hemoglobin Subunit Beta (HBB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. PGL4.11-COL1A1double mutants

   Plasmid

   #230918

   Purpose

   Double mutation of TEAD and SMAD binding elements in COL1A1 promoter; check the promoter activity.

   Depositor

   Avi Rosenberg

   Article

   Yang et al Lab Invest. 2024 May;104(5):100336. doi:

   Insert

   Human CLO1A1 promoter including SMAD and TEAD mutant elements (COL1A1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase-luc2p

   Expression

   Bacterial and Mammalian

   Mutation

   COL1A1 promoter with mutant SMAD and TEAD binding…

   Promoter

   COL1A1 with mutant SMAD and TEAD elements

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. MADR pDonor smFP-FLAG (dark) WPRE

   Plasmid

   #131005

   Purpose

   MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-FLAG, a cytoplasmic fluorescent protein which includes multiple FLAG epitope tags

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   smFP-FLAG WPRE
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   FLAG - 10 total FLAG tags

   Expression

   Mutation

   "dark" variant with GGG fluorphore comp…

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Nsp3 Mac1 - C2 (UCSF)

   Plasmid

   #169209

   Purpose

   Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

   Depositor

   James S Fraser

   Article

   Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

   Insert

   SARS-CoV-2 Nsp3 Mac1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. Nsp3 Mac1 - P43 (Oxford)

   Plasmid

   #169210

   Purpose

   Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

   Depositor

   James S Fraser

   Article

   Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

   Insert

   SARS-CoV-2 Nsp3 Mac1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. PB-EEA-5g-OSK2M2L1-PGK-Puro

   Plasmid

   #102909

   Purpose

   PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. GG-EBNA-OMKSL-PP

   Plasmid

   #102899

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   OMKSL-gRNAS-PGK-puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-StrepII (Human autophagy E3-like enzyme)

   Plasmid

   #169076

   Purpose

   Insect codon optimized ATG genes (excluded tags):10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-TEV-StrepII

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Inserts

   ATG5 (ATG5 Human)
   ATG7 (ATG7 Human)
   ATG10 (ATG10 Human)
   ATG12 (ATG12 Human)
   ATG16 (ATG16L1 Human)
   

   Use

   Tags

   GGlinker, TEV cleavage site, StrepII and HIS10 Ta…

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Polyhedrin promoter

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)

   Plasmid

   #169077

   Purpose

   Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Inserts

   ATG5 (ATG5 Human)
   ATG7 (ATG7 Human)
   ATG10 (ATG10 Human)
   ATG12 (ATG12 Human)
   ATG16 (ATG16L1 Human)
   

   Use

   Tags

   GFP tag, GGlinker, TEV cleavage site. StrepII and…

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Polyhedrin promoter

   Available since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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