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Plasmid#211712PurposeExpresses fl. MBD2 GFP tagged in insect cellsDepositorInsertMBD2
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mdn1-FL
Plasmid#171602PurposeExpresses full length S. pombe Mdn1 in insect cells.DepositorInsertMdn1
UseTagsHis6 tagExpressionInsectMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQadd1F
Plasmid#135660PurposeClostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem in the forward (sense) orientation. Retarget intron to insert double-lox site onto genome at desired site.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQadd1R
Plasmid#135661PurposeClostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem, in the reverse (antisense) orientation. Retarget intron to insert double-lox site at desired location.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB HTB_hMSH6_T1219D
Plasmid#135466PurposeExpression human MSH6_T1219D mutant protein using baculovirusDepositorInserthuman MSH6_T1219D (MSH6 Human)
UseTagsHis-tagExpressionInsectMutationchanged threonine to aspartic acidPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Halo-TCAB1 HRD
Plasmid#207535PurposeHomologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB m Rosella I
Plasmid#71247Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.DepositorInsertRosella
UseTagsyeast mitochondrial targeting sequence of citrate…ExpressionYeastMutationPromoterPGK1Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGE347
Plasmid#153228PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pAGM55273
Plasmid#153211PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nrxn3b-Nav1.6-MKII-f-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16
Plasmid#163031PurposeFLiCRE TF with fast kinetics for neuronal expressionDepositorInsertNrxn3b-MKII-f-hLOV1-TEVcs-tTA
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM55261
Plasmid#153210PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
ULK1-Halo HRD
Plasmid#207548PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cellsDepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFL-EGFP-3xFlag-DEST
Plasmid#134903PurposeBaculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tagDepositorTypeEmpty backboneUseTags3x-FlagExpressionInsectMutationPromoterpolyhedronAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
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CD4-MKII-f-hLOV1-TEVcs(ENLYFQ/M)- Gal4
Plasmid#163027PurposeFLiCRE TF with fast kinetics for HEK293T expressionDepositorInsertCD4-MKII-f-hLOV1-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFB-Flag-MyoD-E12
Plasmid#25990DepositorInsertMyoD-E12 Heterodimer Fusion (Myod1 Mouse)
UseBaculovirus transfer vectorTagsFlagExpressionMutationPromoterAvailable sinceSept. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast
Plasmid#213117PurposeExpression of a faster-running MinE mutant in mammalian cellsDepositorInsertMinE(L4E)-EGFP (minE Escherichia coli)
UseLentiviralTagsExpressionMammalianMutationL4EPromoterEF1aAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGE666
Plasmid#153231PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsGFPExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only