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We narrowed to 17,701 results for: puromycin

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Showing: 2361 - 2380 of 17701 results

  1. pMXs-IP-mCherry-TOSI

    Plasmid
    #172496
    Purpose
    mCherry-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.
    Depositor
    David Scadden
    Publication
    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:
    Insert
    PDCD4 (Pdcd4 Mouse)
    Use
    Retroviral
    Tags
    Mcherry
    Expression
    Mammalian
    Promoter
    MLV LTR
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172496-plasmid-map-sequence-id-338550

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  5. pSCMV-H2B-FT-Fast

    Plasmid
    #157669
    Purpose
    Retroviral vector for expressing chimeric human histone H2B fused with the "Fast" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.
    Depositor
    Shangqin Guo
    Publication
    Eastman et al Cell Rep. 2020 Jun 23;31(12):107804. doi
    Insert
    Histone H2B
    Use
    Retroviral
    Tags
    Fast-FT
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    157669-plasmid-map-sequence-id-308111

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  7. pGBW-m4134899

    Plasmid
    #151902
    Purpose
    Mammalian expression plasmid for Human coronavirus OC43 nucleocapsid
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Human coronavirus OC43 nucleocapsid protein (N Human coronavirus OC43)
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    151902-plasmid-map-sequence-id-300220

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  9. pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139094
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139094-plasmid-map-sequence-id-273891

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  14. GG-EBNA-EEA-5guides-PGK-Puro

    Plasmid
    #102898
    Purpose
    EBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EEA-5guides-PGK-Puro
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102898-plasmid-map-sequence-id-198364

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  15. pEM705 Gsi (Ser)

    Plasmid
    #109352
    Purpose
    GsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)
    Depositor
    Robert Lucas
    Publication
    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gsi chimera
    Tags
    None
    Expression
    Mammalian
    Mutation
    Changed Cys at position 391 to Serine (Pertussis …
    Promoter
    CAG
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    109352-plasmid-map-sequence-id-210153

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  18. U6-hGRIN2B-CAG-ps-SpCas9

    Plasmid
    #102851
    Purpose
    A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing
    Depositor
    Michael Lin
    Publication
    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3X Flag and NLS
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    102851-plasmid-map-sequence-id-198995

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Showing: 2361 - 2380 of 17701 results