We narrowed to 1,134 results for: fret

1. mCit-4aa-SNAP25B

   Plasmid

   #53235

   Purpose

   Fluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   SNAP-25B (Snap25 Rat)
   

   Tags

   4aa-linker and mCitrine

   Expression

   Mammalian

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCer-TG2-YFP

   Plasmid

   #85471

   Purpose

   TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET

   Depositor

   Jeffrey Keillor, Ray Truant

   Article

   Caron et al PLoS One. 2012;7(8):e44159. doi: 10.1371

   Insert

   hTG2 (TGM2 Human)
   

   Tags

   EYFP and mCerulean

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pH7m34GW-35S:SNACS

   Plasmid

   #203576

   Purpose

   Plant SnRK2 kinase activity FRET sensor in plant expression vector

   Depositor

   Julian Schroeder

   Article

   Zhang et al eLife. 2020 May 28;9:e56351. doi: 10.755

   Insert

   SNACS
   

   Tags

   StrepII

   Expression

   Plant

   Mutation

   none

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Venus-StagRFP-AktAR

   Plasmid

   #173020

   Purpose

   Yellow-red FRET-based Akt/PKB activity reporter for multiplexed imaging applications.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   Venus-StagRFP-AktAR
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCer-4aa-VAMP2

   Plasmid

   #53233

   Purpose

   Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   VAMP2 (Vamp2 Rat)
   

   Tags

   4aa-linker and mCerulean

   Expression

   Mammalian

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

6. G13 sensor (Ga13_2)

   Plasmid

   #112930

   Purpose

   A FRET sensor for G13 activation, encoded on a single plasmid.

   Depositor

   Dorus Gadella

   Article

   Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

   Insert

   G13 sensor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Dead-Lapronic/pcDNA3.1(-)

   Plasmid

   #140753

   Purpose

   FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio, Negative Control

   Depositor

   Luis Felipe Barros

   Article

   Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

   Insert

   Dead-Lapronic
   

   Expression

   Mammalian

   Mutation

   Changed Histidine 92 to Alanine

   Promoter

   CMV

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Stx1a-12aa-mCer

   Plasmid

   #53236

   Purpose

   Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   Syntaxin 1A (Stx1a Rat)
   

   Tags

   12aa-linker and mCerulean

   Expression

   Mammalian

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-Tsapphire-StagRFP-SrcAR

   Plasmid

   #173018

   Purpose

   Long-Stokes-shift green-red FRET-based Src activity reporter for multiplexed imaging applications.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   Tsapphire-StagRFP-SrcAR
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. peZinCh-2

    Plasmid

    #70008

    Purpose

    expresses eZinCh-2 Zn2+ FRET sensor in the cytosol of mammalian cells

    Depositor

    Maarten Merkx

    Article

    Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

    Insert

    eZinCh-2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pH7m34GW-35S:SNACS S785A

    Plasmid

    #203577

    Purpose

    Plant SnRK2S30A kinase activity FRET sensor in plant expression vector

    Depositor

    Julian Schroeder

    Article

    Zhang et al eLife. 2020 May 28;9:e56351. doi: 10.755

    Insert

    SNACS S785A
    

    Tags

    StrepII

    Expression

    Plant

    Mutation

    S785A

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-NLS-ZapCmR2

    Plasmid

    #59012

    Purpose

    Nuclear zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NLS-ZapCmR2 genetically encoded Zn(II) sensor
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-Lyn-GR-AKAR3

    Plasmid

    #173016

    Purpose

    Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    Lyn-GR-AKAR3
    

    Tags

    EGFP, N-terminal targeting sequence from Lyn kina…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CyPet-A206K-PRL2

    Plasmid

    #149685

    Purpose

    For the FRET-based binding assay between human CNNM3 CBS domain and PRL2.

    Depositor

    Motoyuki Hattori

    Article

    Cai et al Sci Rep. 2020 Jul 30;10(1):12879. doi: 1

    Insert

    CyPet-A206K-PRL2
    

    Tags

    8 histidine tag

    Expression

    Bacterial

    Mutation

    CyPet A206K

    Promoter

    T7

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-NLS-ZapCmR1.1

    Plasmid

    #59011

    Purpose

    Nuclear zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NLS-ZapCmR1.1 genetically encoded Zn(II) sensor
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pJAG98-mTurquoise2-FHA2-AURKB substrate-YPet

    Plasmid

    #83286

    Purpose

    untargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pair

    Depositor

    Daniel Needleman

    Article

    Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

    Insert

    mTurquoise2-FHA2-AURKB substrate-YPet
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPickles-2.31

    Plasmid

    #92336

    Purpose

    FRET-based biosensor for the measurement of BCR-ABL activity

    Depositor

    Yusuke Ohba

    Article

    Mizutani et al Clin Cancer Res. 2010 Aug 1;16(15):3964-

    Insert

    Pickles-2.31
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. H3K9me3 biosensor

    Plasmid

    #120802

    Purpose

    FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. H3S10ph biosensor

    Plasmid

    #120809

    Purpose

    FRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
    

    Expression

    Mammalian

    Mutation

    Threonine 3, Threonine 6, and Threonine 11 are mu…

    Promoter

    CMV

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGS-6011nes

    Plasmid

    #108652

    Purpose

    An ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-ERK-EV
    

    Tags

    XFP

    Expression

    Mammalian

    Promoter

    pCAGGS

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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