We narrowed to 10,515 results for: SEC
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Plasmid#229869PurposeDestination RMCE vector containing FRT::SA::51-bp Artificial exon::3xStop::SL2::SapI Insertion sites (to clone new driver with 3'UTR in slot 5 and SEC in slot 6)::reverse FRT3 to swap driver via RMCEDepositorTypeEmpty backboneExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only
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pMS110
Plasmid#215674PurposeSplit hygromycinR landing pad insertion plasmid for ChrIIIDepositorInsert5'HA + synthetic guide site3 + 3'ΔHygR:unc-54 3' UTR + lox2272 + SEC + lox2272 + 3'HA
UseCRISPR and Cre/LoxExpressionWormMutation3' ∆HYGR is promoterless and encodes aa60-341Available SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSO9
Plasmid#106366PurposeTo insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the intestine.DepositorInsertttTi4348_ SEC _pvit-6_OspF_3'unc54
ExpressionWormAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSALECTNK-TEM1(S70A,D179G)/csTEM1
Plasmid#81163PurposepSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1DepositorInsertTEM-1 beta-lactamase
TagsCodon swapped TEM-1 from H26 to W290 and His6xExpressionBacterialMutationDeleted the sec transport tag. Plasmid encodes H2…Promoterlac promoterAvailable SinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
NDV-HN-SpyTag
Plasmid#160000PurposeExpresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cellsDepositorInsertNDV-HN-SpyTag
TagsHis6-tagExpressionMammalianPromoterChicken beta-actin promoterAvailable SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTT610
Plasmid#101742Purposeencoding SecY(L2V, R252G)–(GGSG)4–SecA1-939–His10 fusion protein and SecE and SecG from Thermus thermophilus HB8DepositorTagsHis10ExpressionBacterialMutationSecY(L2V, R252G)–(GGSG)4–SecA1-939–His10 fusion p…PromoterLacAvailable SinceNov. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAK22
Plasmid#101741Purposeencoding two copies of T. thermophilus SecG for expression of SecYEG complexDepositorInsertThermus thermophilus HB8 SecG x 2
TagsNONEExpressionBacterialPromoterT7Available SinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
MUNC18a
Plasmid#218888PurposeExpresses rat MUNC18a in bacteriaDepositorAvailable SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf-Btl2
Plasmid#117664PurposeStudy secretion efficiency of Btl2DepositorInsertpre-pro-alphaf-Btl2
ExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf-E2-Crimson
Plasmid#117658PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-pro-alphaf-Btl2
Plasmid#117665PurposeStudy secretion efficiency of Btl2DepositorInsertpre-Ost1-pro-alphaf-Btl2
ExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf(I)-E2-Crimson
Plasmid#117660PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf(I)-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson
Plasmid#117662PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpOst1-pro-af(MUT1)-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson
Plasmid#117663PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpOst1-pro-af(MUT2)-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-alphaf(I)-E2-Crimson
Plasmid#117661PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-Ost1-alphaf(I)-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-alphaf(I)-Btl2
Plasmid#117667PurposeStudy secretion efficiency of Btl2DepositorInsertpre-Ost1-alphaf(I)-Btl2
ExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJW1816
Plasmid#154320PurposeMulti-cassette for SapTrapDepositorInsert30aa linker::mScarlet-I^SEC (Lox511I)^3xMyc
UseCRISPR and Cre/LoxExpressionWormAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1846
Plasmid#154326PurposeMulti-cassette for SapTrapDepositorInsert30aa linker::GFP^SEC^AID*::3xFLAG
UseCRISPR and Cre/LoxExpressionWormMutationF+E mutations in sgRNAAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1820
Plasmid#154321PurposeMulti-cassette for SapTrapDepositorInsertGFP^SEC (LoxP)^AID*::3xFLAG
UseCRISPR and Cre/LoxExpressionWormAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1886
Plasmid#154333PurposeMulti-cassette for SapTrapDepositorInsert30aa linker::mScarlet-I^SEC (Lox511I)^TEV::degron::3xFLAG
UseCRISPR and Cre/LoxExpressionWormAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
NST3_pOpOn2.1
Plasmid#228066PurposeExpresses NST3 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.DepositorInsertNAC SECONDARY WALL THICKENING PROMOTING FACTOR3, NST3 (NAC012 Mustard Weed)
UseDexamethasone inducible vectorExpressionPlantPromoterCaMV35S for LhGR and pOp6-minimal CaMV35S for NST3Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTa_SYK
Plasmid#224246PurposeExpression of human SYK Kinase in insect cells (SF9 cells)DepositorAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTa_HCK
Plasmid#224245PurposeExpression of human HCK Kinase in insect cells (SF9 cells)DepositorAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTa_LYN
Plasmid#224244PurposeExpression of human LYN Kinase in insect cells (SF9 cells)DepositorAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
R777-E077 Hs.EXOC6
Plasmid#70361PurposeGateway ORF clone of human EXOC6 [NM_019053.4] with stop codon (for native or N-terminal fusions)DepositorInsertEXOC6 (EXOC6 Human)
UseGateway entry cloneAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E078 Hs.EXOC6-nostop
Plasmid#70362PurposeGateway ORF clone of human EXOC6 [NM_019053.4] without stop codon (for C-terminal fusions)DepositorInsertEXOC6 (EXOC6 Human)
UseGateway entry cloneAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a207-368.wt
Plasmid#13531DepositorInsertB. subtilis DEAD-box helicase YxiN second domain
Tagsintein-chitin binding domainExpressionBacterialMutationdeleted amino acid residues 1-206 and 369-479Available SinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-PA
Plasmid#175446PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneTags3C-Protein A and BiP Secretion PeptideExpressionInsectPromoterFused Actin-HSP70Available SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-His-zfTpo
Plasmid#64309PurposeExpress soluble His-tagged zebrafish thrombopoietin in insect cellsDepositorInsertThrombopoietin (thpo Zebrafish)
Tags(His)6ExpressionInsectMutationThrombopoietin starts at AA37 (AA1-36 encode the …Available SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-10H-SIII
Plasmid#175444PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneTags3C-10His-TwinStrep and BiP Secretion PeptideExpressionInsectPromoterFused Actin-HSP70Available SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-PA-10H-SIII
Plasmid#175443PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneTags3C-Protein A-10His-TwinStrep and BiP Secretion Pe…ExpressionInsectPromoterFused Actin-HSP70Available SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-CR-PA
Plasmid#175445PurposeHiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneTagsBiP Secretion Peptide and Thrombin-Protein AExpressionInsectPromoterFused Actin-HSP70Available SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-CR-PA-10H-SIII
Plasmid#175447PurposeHiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneTagsBiP Secretion Peptide and Thrombin-Protein A-10Hi…ExpressionInsectPromoterFused Actin-HSP70Available SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
NST1_pOpOn2.1
Plasmid#228065PurposeExpresses NST1 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.DepositorInsertNAC SECONDARY WALL THICKENING PROMOTING FACTOR1, NST1 (NST1 Mustard Weed)
UseDexamethasone inducible vectorExpressionPlantPromoterCaMV35S for LhGR and pOp6-minimal CaMV35S for NST1Available SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-sRGECO
Plasmid#137128PurposeIntersectional viral expression of sRGECO in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-sRGECO
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationE217DPromoterEF1aAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-mCherry
Plasmid#137134PurposeIntersectional viral expression of mCherry in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-mCherry
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationn/aPromoterEF1aAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Con/Fon-GCaMP6M
Plasmid#137119PurposeIntersectional viral expression of GCaMP6M in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-GCaMP6M
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationRemoved RSET tagPromoterEF1aAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-GCaMP6F
Plasmid#137122PurposeIntersectional viral expression of GCaMP6F in cells expressing both Cre AND FlpDepositorHas ServiceAAV5 and AAV8InsertCon/Fon-GCaMP6F
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationRemoved RSET tagPromoterEF1aAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-mCherry
Plasmid#137132PurposeIntersectional viral expression of mCherry in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-mCherry
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationn/aPromoterEF1aAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Foff 2.0-EYFP
Plasmid#137162PurposeIntersectional viral expression of EYFP in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationn/aPromoterEF1aAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only