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Sirius-mT-Sapphire_pcDNA3

Plasmid

#89584

Purpose

Genetically encoded ratiometric fluorescent thermometer

Depositor

Takeharu Nagai

Article

Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

Insert

Sirius-mT-Sapphire

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pUCP20T-tsapphire

Plasmid

#78464

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Article

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

T-Sapphire

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-DsRed_IRES_SNCA:EGFP

Plasmid

#92195

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for α-Syn screen

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-SNCA-EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only
PU.1-IRES-mCherry

Plasmid

#80140

Purpose

retroviral expression of PU.1 and mCherry

Depositor

Ellen Rothenberg

Article

Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

Insert

PU.1 (Spi1 Mouse)

Use

Retroviral

Tags

IRES-mCherry

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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HRV-B3 IRES

Plasmid

#189969

Purpose

Part 2 plasmid to introduce the HRV-B3 IRES into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

HRV-B3 IRES

Use

Cloning

Tags

Expression

Mutation

Promoter

N/A

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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LZRS-hNEDD9_IresGFP

Plasmid

#21962

Depositor

Lynda Chin

Article

Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

Insert

NEDD9 (NEDD9 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
CAG-NeuroD1iresGFP

Plasmid

#45025

Depositor

Connie Cepko

Article

Cherry et al J Neurosci. 2011 May 18;31(20):7365-79.

Insert

NeuroD1 (Neurod1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

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pLARRYv2-T-Sapphire

Plasmid

#233209

Purpose

Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with T-Sapphire marker

Depositor

Alejo Rodriguez-Fraticelli

Article

Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sapphireCKAR

Plasmid

#118468

Purpose

cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireCKAR

Use

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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mT-Sapphire-pBAD

Plasmid

#54571

Purpose

Localization: Protein Expression Vector , Excitation: 399, Emission: 511

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al Nat Methods. 2008 May . 5(5):401-3.

Type

Empty backbone

Use

Tags

6xHis and mT-Sapphire

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
LZRS-IresGFP

Plasmid

#21961

Depositor

Lynda Chin

Article

Kim et al Cell. 2006 Jun 30. 125(7):1269-81.

Insert

Ires GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

GACAGATGACTATGCAGAGAT

Promoter

Available since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only
Jag1-HA pIRES

Plasmid

#17336

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Jagged1 (Jag1 Rat)

Use

Tags

HA X3

Expression

Mammalian

Mutation

A silent C->G mutation at 3237

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only

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pCG_IFITM3-IRES_BFP

Plasmid

#179960

Purpose

Expression vector for human IFITM3. Co-expresses BFP controlled by an IRES element.

Depositor

Konstantin Sparrer, Frank Kirchhoff

Article

Prelli Bozzo et al Nat Commun. 2021 Jul 28;12(1):4584.

Insert

IFITM3 (IFITM3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 sgIREB2_1

Plasmid

#169894

Purpose

Disrupt IREB2

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting IREB2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES2-hPAC

Plasmid

#129488

Purpose

Expresses human TMEM206

Depositor

Zhaozhu Qiu

Article

Yang et al Science. 2019 Apr 26;364(6438):395-399.

Insert

Transmembrane protein 206 (PACC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCG_IFITM1-IRES_eGFP

Plasmid

#179958

Purpose

Expression vector for human IFITM1. Co-expresses GFP controlled by an IRES element.

Depositor

Konstantin Sparrer, Frank Kirchhoff

Article

Prelli Bozzo et al Nat Commun. 2021 Jul 28;12(1):4584.

Insert

IFITM1 (IFITM1 Human)

Use

Tags

Expression

Mammalian

Mutation

P13A

Promoter

Available since

Feb. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSiren_EGFP-shLuc

Plasmid

#122287

Purpose

Knockdown control

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Knockdown shRNA for Luc

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pIRES-HA-14-3-3 ϵ/myc-14-3-3ζ

Plasmid

#48795

Purpose

expresses human HA-14-3-3 ϵ (epsilon) and myc-14-3-3ζ (zeta) in mammalian cells

Depositor

Huda Zoghbi

Article

Chen et al Cell. 2003 May 16;113(4):457-68.

Inserts

YWHAE (YWHAE Human)
YWHAZ (YWHAZ Human)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV and CMV (IRES)

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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pIRESNEO-Scambio-HA

Plasmid

#33354

Depositor

John Groffen, Nora Heisterkamp

Article

Curtis et al Mol Cancer. 2004 Apr 23;3:10.

Insert

Scambio (ARHGEF40 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
StrKDEL-IRES_mScarleti_Sec23A

Plasmid

#117273

Purpose

Expresses an Str-KDEL Hook for the RUSH system and separately mScarleti-Sec23A

Depositor

David Stephens

Article

McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

Inserts

SEC23A (SEC23A Human)
Streptavidin-KDEL

Use

Tags

mScarlet-i

Expression

Mammalian

Mutation

Human Sec23A; Sequencing showed a mutation in Sec…

Promoter

CMV and IRES

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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