Addgene

Showing: 221 - 240 of 16701 results

Results per page:

Sort results by:

tdEos-Talin-18

Plasmid

#57672

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
tdEos-Paxillin-22

Plasmid

#57653

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Paxillin

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pGJJ055

Plasmid

#183622

Purpose

ddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies.

Depositor

Ben Lehner

Article

Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mEos2-VASP-5

Plasmid

#57433

Purpose

Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

VASP

Use

Tags

mEos2

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
tdEos-Paxillin-KM-C-10

Plasmid

#57654

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Paxillin KM

Use

Tags

tdEos

Expression

Mammalian

Mutation

Mutation to remove Kozak sequence

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Integrin-β6 I domain I270C

Plasmid

#159639

Purpose

Contains integrin beta 6 residues 108–352 with I270C mutation

Depositor

Timothy Springer

Article

Dong et al Nature. 2017 Feb 2;542(7639):55-59. doi:

Insert

Integrin beta 6 I domain I270C

Use

Tags

6x His

Expression

Mammalian

Mutation

I270C

Promoter

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mEos2-Zyxin-6

Plasmid

#57441

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Zyxin (ZYX Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
tdEos-FAK-5

Plasmid

#57612

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

FAK

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-Talin-R-15

Plasmid

#57423

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin R

Use

Tags

mEos2

Expression

Mammalian

Mutation

Talin Rod Domain

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pKLBac-Bat-FluA-trimer

Plasmid

#229858

Purpose

Influenza A/little yellow-shouldered bat/Guatemala/060/2010(H17N10) polymerase heterotrimer (self-cleaving polyprotein)

Depositor

Stephen Cusack

Article

Pflug et al Nature. 2014 Dec 18;516(7531):355-60. do

Inserts

PA_Bat-FluA_AFC35437.1
PB1_Bat-FluA_AFC35436
PB2_Bat-FluA_AFC35435.1

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTH1-SaSrtA-A

Plasmid

#64979

Purpose

Expresses the E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Use

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E108A

Promoter

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pTH1-SaSrtA-K

Plasmid

#64978

Purpose

Expresses the E105K mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Use

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K

Promoter

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Pediculus humanus corporis PINK1 (115 - 575)

Plasmid

#110750

Purpose

Bacterial expression of PhPINK1 (biochemistry)

Depositor

David Komander

Article

Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

Insert

Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)

Use

Tags

N-His6-GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
xCas9 3.7

Plasmid

#108379

Purpose

Mammalian expression vector for xCas9 3.7

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9 3.7

Use

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
piggyBAC-5xTAPE-1-BlastR

Plasmid

#175808

Purpose

Encodes 5xTAPE-1 construct that can be integrated using piggyBAC system

Depositor

Jay Shendure

Article

Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

Insert

5xTAPE-1 construct

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-5'-ENGRAM

Plasmid

#179157

Purpose

piggyBac plasmid for 5' ENGRAM recorder

Depositor

Jay Shendure

Article

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

minP

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGJJ045

Plasmid

#183621

Purpose

abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.

Depositor

Ben Lehner

Article

Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
xCas9 3.6

Plasmid

#108384

Purpose

Mammalian expression vector for xCas9 3.6

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9 3.6

Use

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Promoter

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MSP2283

Plasmid

#70702

Purpose

Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
MSP2266

Plasmid

#70705

Purpose

Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge