Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 6,178 results for: cas9 expression plasmid

Clear keywords
Showing: 221 - 240 of 6178 results

  1. pTRE3G-dCas9-VP64-MS2-VP64-ZsG

    Plasmid
    #192669
    Purpose
    Dox-inducible expression of dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    TRE3G
    Available Since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192669-plasmid-map-sequence-id-379887

    Enlarge

  2. lenti-EF1a-dCas9-VP64-H2B-GFP

    Plasmid
    #242164
    Purpose
    Lentiviral expression of dCas9-VP64 with H2B-GFP reporter
    Depositor
    Amy Brock
    Insert
    dCas9-VP64; T2A-H2B-GFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242164-plasmid-map-sequence-id-496078

    Enlarge

  3. lenti-EF1a-dCas9-KRAB-H2B-mCherry

    Plasmid
    #242167
    Purpose
    Lentiviral expression of dCas9-KRAB with with H2B-mCherry reporter
    Depositor
    Amy Brock
    Insert
    dCas9-KRAB; T2A-H2B-mCherry
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242167-plasmid-map-sequence-id-496074

    Enlarge

  4. lenti-EF1a-dCas9-KRAB-H2B-GFP

    Plasmid
    #242166
    Purpose
    Lentiviral expression of dCas9-KRAB with H2B-GFP reporter
    Depositor
    Amy Brock
    Insert
    dCas9-KRAB; T2A-H2B-GFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242166-plasmid-map-sequence-id-496073

    Enlarge

  5. pLentiV2-dCas9-VP64

    Plasmid
    #141104
    Purpose
    Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa
    Depositor
    Igor Ulitsky
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141104-plasmid-map-sequence-id-298612

    Enlarge

  6. dCas9-miniTurbo

    Plasmid
    #219822
    Purpose
    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Insert
    dCas9
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    EF1a
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219822-plasmid-map-sequence-id-482202

    Enlarge

  10. pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA

    Plasmid
    #113692
    Purpose
    U6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 (NEWENTRY )
    SaCas9 gRNA Cassette (NEWENTRY )
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    NLS
    Promoter
    Cytomegalo Virus(CMV)
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113692-plasmid-map-sequence-id-229557

    Enlarge

  11. lenti-EF1a-dCas9-KRAB-MeCP2-Puro

    Plasmid
    #242163
    Purpose
    Lentiviral expression of dCas9-KRAB-MeCP2 with puromycin reporter
    Depositor
    Amy Brock
    Insert
    dCas9-KRAB-MeCP2; T2A-puro
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242163-plasmid-map-sequence-id-496076

    Enlarge

  12. lenti-EF1a-dCas9-VPR-H2B-GFP

    Plasmid
    #242168
    Purpose
    Lentiviral expression of dCas9-VPR with H2B-GFP reporter
    Depositor
    Amy Brock
    Insert
    dCas9-VP64-p65/RelA-Rta; T2A-H2B-GFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242168-plasmid-map-sequence-id-496079

    Enlarge

  13. lenti-EF1a-dCas9-VPR-H2B-mCherry

    Plasmid
    #242169
    Purpose
    Lentiviral expression of dCas9-VPR with H2B-mCherry reporter
    Depositor
    Amy Brock
    Insert
    dCas9-VP64-p65/RelA-Rta; T2A-H2B-mCherry
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242169-plasmid-map-sequence-id-496075

    Enlarge

  14. SIN-cPPT-G1B3-spCas9-WPRE-miR124T

    Plasmid
    #245071
    Purpose
    CRISPR Editing with SpCas9, with miRNA-124 target sequence to repress transgene expression in neurons
    Depositor
    Nicole Déglon
    Insert
    Cas9, miR124T site
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Human G1B3 promoter
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245071-plasmid-map-sequence-id-495712

    Enlarge

  15. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid
    #174140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174140-plasmid-map-sequence-id-341918

    Enlarge

  19. AAVS1-idCas9-vpr

    Plasmid
    #89985
    Purpose
    inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus
    Depositor
    Jie Na
    Article
    Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/
    Insert
    dCas9-VPR
    Expression
    Mammalian
    Promoter
    TRE-tight
    Available Since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    89985-plasmid-map-sequence-id-180638

    Enlarge

  20. pDual_dsCas9_Puro_hs_NC_chr1

    Plasmid
    #214688
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Mutation
    Puromycin resistance cassette has silent mutation…
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214688-plasmid-map-sequence-id-428817

    Enlarge

Showing: 221 - 240 of 6178 results