We narrowed to 23,940 results for: nes

1. p3XP3-WhiteA2

   Plasmid

   #165875

   Purpose

   Basic transgenesis 3xP3 white CDS physical eye marker cassette in Alpha2, lacks attB site

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Eye Marker Cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TET-pLKO.1 PURO shLacZ

   Plasmid

   #98632

   Purpose

   Lentiviral shRNA vector for inducible knockdown of LacZ (control hairpin)

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   shLacZ
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDGB3_alpha1

   Plasmid

   #68228

   Purpose

   GoldenBraid level 1 Alpha plant expression vector.

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   empty vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMaCTag-Z09

   Plasmid

   #120052

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mVenus

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mVenus
   

   Use

   Pcr template

   Tags

   mVenus

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX330A_D10A-1x4

   Plasmid

   #58774

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDsRed (GB0100)

   Plasmid

   #68202

   Purpose

   Provides the CDS of DsRed as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   DsRed coding region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMaCTag-Z33

   Plasmid

   #120050

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with FLAG

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   FLAG
   

   Use

   Pcr template

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMaCTag-Z17

   Plasmid

   #120060

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mEos4b

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mEos4b
   

   Use

   Pcr template

   Tags

   mEos4b

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CeNL(Ca2+)_1.2μ-pcDNA3

   Plasmid

   #111926

   Purpose

   Cyan color bright luminescent calcium indicator with intermediate affinity

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   CeNL(Ca2+)_1.2μ
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E67D, E104D, D133E at CaM

   Promoter

   CMV

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Nano-lantern(Ca2+)_300/pcDNA3

    Plasmid

    #51983

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-3GS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX330A_D10A-1x6

    Plasmid

    #58776

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMaCTag-P14

    Plasmid

    #120025

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeptune2.5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mNeptune2.5
    

    Use

    Pcr template

    Tags

    mNeptune2.5

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMaCTag-Z15

    Plasmid

    #120058

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with miRFP670

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    miRFP670
    

    Use

    Pcr template

    Tags

    miRFP670

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. OeNL(Ca2+)_18μ-pcDNA3

    Plasmid

    #111928

    Purpose

    Orange color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    OeNL(Ca2+)_18μ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT7-Venus-Emi1 (914)

    Plasmid

    #39854

    Depositor

    Jonathon Pines

    Article

    Di Fiore et al J Cell Biol. 2007 May 7;177(3):425-37.

    Insert

    Emi1 (FBXO5 Human)
    

    Use

    In vitro transcription

    Tags

    Venus

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMaCTag-P15

    Plasmid

    #120026

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    miRFP670
    

    Use

    Pcr template

    Tags

    miRFP670

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTRF.1-mCMV-udsVenus

    Plasmid

    #58518

    Purpose

    Empty lentiviral expression vector for ultradestabilized Venus that indicates the activity of a transcriptional element of interest

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    None
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMaCTag-P24

    Plasmid

    #120035

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TAP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    TAP
    

    Use

    Pcr template

    Tags

    TAP

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMaCTag-P10

    Plasmid

    #120021

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOk

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mKOk
    

    Use

    Pcr template

    Tags

    mKOk

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CeNL(Ca2+)_19μ-pcDNA3

    Plasmid

    #111927

    Purpose

    Cyan color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_19μ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E67D, F92W, E104D, D133E at CaM

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Nano-lantern(Ca2+)_600/pcDNA3

    Plasmid

    #51982

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-2G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pMaCTag-P18

    Plasmid

    #120029

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKeima8.5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mKeima8.5
    

    Use

    Pcr template

    Tags

    mKeima8.5

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMaCTag-Z08

    Plasmid

    #120051

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mClover3

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mClover3
    

    Use

    Pcr template

    Tags

    mClover3

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pENTR221-Firefly

    Plasmid

    #79495

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Firefly Luciferase CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    Firefly Luciferase
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCLC-mEos2

    Plasmid

    #38010

    Depositor

    Xiaowei Zhuang

    Article

    Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Use

    Tags

    mEos2

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENTR221-Renilla

    Plasmid

    #79496

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    Renilla Luciferase
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMC-1-AtU6-Y

    Plasmid

    #197779

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    AtU6 promoter for sgRNA assembly
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMC-1-OsU6-X

    Plasmid

    #197780

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    OsU6 promoter for sgRNA assembly
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMC-Z-SpCas9-gRNA-repeat-polyT-11

    Plasmid

    #197781

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    gRNA repeat for U6 sgRNA assembly
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 7iBi89 (rat fibronectin)

    Plasmid

    #14065

    Depositor

    Richard Hynes

    Article

    Huh et al Mol Cell Biol. 1993 Sep . 13(9):5301-14.

    Insert

    Rat Fibronectin 7iBi89 (Fn1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Insert was cut out of pGEM3 with SalI and SacI an…

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only