Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 14,600 results for: pET

Showing: 4761 - 4800 of 14600 results

  1. pZF-BCB-PDZ

    Plasmid
    #127478
    Purpose
    coding sequence for synthetic zinc finger ZF-BCB-PDZ
    Depositor
    Sebastian Maerkl
    Article
    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11
    Insert
    ZF-BCB synthetic transcription factor fused with the alpha 1-syntrophin PDZ domain
    Use
    Cell-free transcription–translation
    Tags
    alpha 1-syntrophin PDZ domain
    Expression
    Mutation
    Promoter
    T7
    Available since
    July 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    127478-plasmid-map-sequence-id-248851

    Enlarge

  2. pZF-ADD-L

    Plasmid
    #127477
    Purpose
    coding sequence for synthetic zinc finger ZF-ADD-L
    Depositor
    Sebastian Maerkl
    Article
    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11
    Insert
    ZF-ADD synthetic transcription factor fused with PDZ cognate peptide ligand (VKESLV)
    Use
    Cell-free transcription–translation
    Tags
    PDZ cognate peptide ligand (VKESLV)
    Expression
    Mutation
    Promoter
    T7
    Available since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127477-plasmid-map-sequence-id-263301

    Enlarge

  5. pAM57

    Plasmid
    #227654
    Purpose
    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, V80A)
    Depositor
    Andreas Martin
    Article
    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-
    Inserts
    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    Use
    Tags
    H6-PreScission and PreScission-Strep
    Expression
    Bacterial
    Mutation
    1-179 and amino acids 2-239, V80A
    Promoter
    T7
    Available since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227654-plasmid-map-sequence-id-455272

    Enlarge

  6. pAM54

    Plasmid
    #227653
    Purpose
    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, L132A)
    Depositor
    Andreas Martin
    Article
    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-
    Inserts
    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    Use
    Tags
    H6-PreScission and PreScission-Strep
    Expression
    Bacterial
    Mutation
    1-179 and amino acids 2-239, L132A
    Promoter
    T7
    Available since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227653-plasmid-map-sequence-id-455251

    Enlarge

  8. pAM71

    Plasmid
    #227621
    Purpose
    pETDuet-1 H6-precission-Rpn11(24-160-GGSGSG-192-221) , Rpn8(1-179)
    Depositor
    Andreas Martin
    Article
    Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d
    Inserts
    Rpn11 (RPN11 Budding Yeast)
    rpn8 (RPN8 Budding Yeast)
    Use
    Tags
    His-Prescission
    Expression
    Bacterial
    Mutation
    1-179 and amino acids 24-160-GGSGSG-192-221
    Promoter
    T7
    Available since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227621-plasmid-map-sequence-id-455206

    Enlarge

  13. pHIT348

    Plasmid
    #204515
    Purpose
    Bacterial expression of CEC-5 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    C-terminal fragment of cec-5 (C. elegans)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204515-plasmid-map-sequence-id-411110

    Enlarge

  15. pHIT334

    Plasmid
    #204516
    Purpose
    Bacterial expression of COH-3 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    C-terminal fragment of coh-3 (C. elegans)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    204516-plasmid-map-sequence-id-403820

    Enlarge

  16. pHIT186

    Plasmid
    #204513
    Purpose
    Bacterial expression of CSR-1 PAZ domain with 6xHis and maltose binding protein tags for use as an antigen
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    PAZ domain of csr-1 (C. elegans) (csr-1 Nematode)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    204513-plasmid-map-sequence-id-403809

    Enlarge

  17. pHIT521

    Plasmid
    #204520
    Purpose
    Bacterial expression of CEC-5 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    N-terminal fragment of cec-5 (C. elegans)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204520-plasmid-map-sequence-id-403749

    Enlarge

  18. pHIT182

    Plasmid
    #204511
    Purpose
    Bacterial expression of catalytically defective CSR-1a with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Article
    Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.
    Insert
    csr-1a with D769A mutation (C. elegans) (csr-1 Nematode)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    D769A
    Promoter
    T7lac
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204511-plasmid-map-sequence-id-403747

    Enlarge

  19. pHIT520

    Plasmid
    #204519
    Purpose
    Bacterial expression of CEC-8 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    N-terminal fragment of cec-8 (C. elegans) (Y55B1BR.3 Nematode)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204519-plasmid-map-sequence-id-403744

    Enlarge

  20. pHIT342

    Plasmid
    #204514
    Purpose
    Bacterial expression of SYP-1 N-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    N-terminal fragment of syp-1 (C. elegans)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204514-plasmid-map-sequence-id-403743

    Enlarge

  21. pHIT320

    Plasmid
    #204517
    Purpose
    Bacterial expression of HIM-1 internal fragment with 6xHis and maltose binding protein tags for use as an antigen
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    Internal fragment of him-1 (C. elegans) (him-1 Nematode)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204517-plasmid-map-sequence-id-403742

    Enlarge

  22. pSLS.539

    Plasmid
    #184958
    Purpose
    Test effect of a1/a2 length on editing fabH using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12
    Use
    Tags
    Expression
    Bacterial
    Mutation
    fabH donor G954A
    Promoter
    T7/lac
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    184958-plasmid-map-sequence-id-381448

    Enlarge

  24. pSLS.543

    Plasmid
    #184960
    Purpose
    Test effect of a1/a2 length on editing murF using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
    Use
    Tags
    Expression
    Bacterial
    Mutation
    murF donor G1359A
    Promoter
    T7/lac
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184960-plasmid-map-sequence-id-376545

    Enlarge

  25. pSLS.545

    Plasmid
    #184962
    Purpose
    Test effect of a1/a2 length on editing priB using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
    Use
    Tags
    Expression
    Bacterial
    Mutation
    priB donor G1069A
    Promoter
    T7/lac
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184962-plasmid-map-sequence-id-376122

    Enlarge

  27. pPD3cC

    Plasmid
    #86972
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …
    Promoter
    T7 promoter
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86972-plasmid-map-sequence-id-173929

    Enlarge

  40. pSLS.492

    Plasmid
    #184957
    Purpose
    Test effect of a1/a2 length on editing rpoB using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
    Use
    Tags
    Expression
    Bacterial
    Mutation
    rpoB donor T1534C, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184957-plasmid-map-sequence-id-376681

    Enlarge

Showing: 4761 - 4800 of 14600 results