We narrowed to 1,249 results for: vent

1. pBUN501

   Plasmid

   #50582

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   zCas9D10A
   gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   Cas9D10A nickase, was derived from the zCas9 and …

   Promoter

   AtU6-26p and Ubi1p

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cav2.1 EA2, R57A, R59A pMT2

   Plasmid

   #206122

   Purpose

   expression of rabbit Cav2.1 calcium channel with a CaV2.1-P1217fs mutation causing a truncated protein, (EA2). R57A R59A mutations prevent dominant-negative suppression of Cav2.1 currents

   Depositor

   Annette Dolphin

   Article

   Dahimene et al Neurobiol Dis. 2016 Sep;93:243-56. doi:

   Insert

   cacna1a (Cacna1a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cav2.1-P1217fs, R57A, R59A

   Promoter

   Ad MLP/TPL/SV40

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-plk1-AKAP

   Plasmid

   #132967

   Purpose

   To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

   Depositor

   Michael Yaffe

   Article

   Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

   Insert

   polo-like kinase 1-AKAP (PLK1, AKAP9 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C terminus of full-length Plk1 fused to the peric…

   Promoter

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRRLNeo-pEF1a-p53ash-mKate2-splitmVenusN

   Plasmid

   #69582

   Purpose

   Expresses p53 tagged with mKate2 and split N-term mVenus

   Depositor

   Galit Lahav

   Article

   Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

   Insert

   p53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mKate2 and split mVenus - N term

   Expression

   Mammalian

   Mutation

   7 silent mutations in p53 DNA to prevent targetin…

   Promoter

   EF1alpha

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGal-Trans-Myc-HA-GFP11-KDEL

   Plasmid

   #177724

   Purpose

   ER protein collagen beta (1-O) galactosyltransferase with a C-terminal Myc tag, an HA tag and the eleventh β-strand of GFP

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)
   

   Use

   Tags

   GFP11, HA, KDEL, and Myc

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Bn R52A, R54A pMT2

   Plasmid

   #206087

   Purpose

   Cav2.2 N-terminus (amino acids 1-95) with R52A and R54A mutations that prevent dominant-negative suppression of Cav2.2 currents by truncated Cav2.2

   Depositor

   Annette Dolphin

   Article

   Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R52A, R54A

   Promoter

   Ad MLP/TPL/SV40

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cav2.2 W391A pcDNA3

   Plasmid

   #206085

   Purpose

   expression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

   Depositor

   Annette Dolphin

   Article

   Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   W391A

   Promoter

   CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p35S_EVDm1

   Plasmid

   #167121

   Purpose

   Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) with U1 binding site mutated under CaMV 35S promoter.

   Depositor

   Olivier Voinnet, Arturo Marí-Ordóñez

   Article

   Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

   Insert

   EVADE (EVD) (AT5G17125 Mustard Weed)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   mutated four nucleotides at the snoRNA U1 binding…

   Promoter

   CaMV 35S

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA tagged mid ELL2 M133, 138, 186ILeu

   Plasmid

   #127276

   Purpose

   Expresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains middle HA tag that doesn't disrupt function

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (ELL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M133, 138, 186 to Ileu, HA tag at XbaI after Met …

   Promoter

   pEF1a

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUB6_EZH2

    Plasmid

    #84014

    Purpose

    EZH2 minigene to test splicing event

    Depositor

    Robert Bradley

    Article

    Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UB6

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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