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We narrowed to 3,796 results for: Mak

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Showing: 3601 - 3630 of 3796 results

  11. pL0_CE-OriT

    Plasmid
    #202150
    Purpose
    Level 0 plasmid containing an origin of transfer sequence from pPtPBR11 required for making plasmids mobilizable for conjugation with CE overhangs used to build level 1 constructs.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    Origin of transfer
    Use
    Synthetic Biology
    Mutation
    OriT sequence mutated to remove sapI sites domest…
    Available Since
    Aug. 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202150-plasmid-map-sequence-id-405613

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  12. pCDNA3_shRNA resistant PKA-Cα(E12,14K)-mEGFP

    Plasmid
    #168491
    Purpose
    Mammalian expression of the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
    Depositor
    Haining Zhong
    Article
    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11
    Insert
    the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by mEGFP. Resistant to shRNA knock-down.
    Expression
    Mammalian
    Available Since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168491-plasmid-map-sequence-id-332602

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  13. pROS13_IDH2 #7

    Plasmid
    #166102
    Purpose
    This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.
    Depositor
    Matthias Heinemann
    Article
    Chen et al bioRxiv
    Insert
    IDH2 sgRNA #7 (IDH2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    166102-plasmid-map-sequence-id-330316

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  14. pROS13_IDH1 #4

    Plasmid
    #166101
    Purpose
    This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.
    Depositor
    Matthias Heinemann
    Article
    Chen et al bioRxiv
    Insert
    IDH1 sgRNA #4 (IDH1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    166101-plasmid-map-sequence-id-330298

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  21. pCR4-*mRFP

    Plasmid
    #47118
    Purpose
    Can be used to make an RNA probe for whole-mount in situ hybridization, for in-frame fusion proteins or as a subcloning step for mRFP. Note that the coding sequence for mRFP lacks the start codon.
    Depositor
    Stephen Ekker
    Article
    Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub
    Insert
    *mRFP
    Expression
    Bacterial
    Mutation
    No start codon
    Promoter
    Lac
    Available Since
    Oct. 16, 2013
    Availability
    Academic Institutions and Nonprofits only
    47118-plasmid-map-sequence-id-67205

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  27. pcDNA3-PKAsub(T/A)-YPet-NES

    Plasmid
    #138205
    Purpose
    Encodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub(T/A)-YPet-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138205-plasmid-map-sequence-id-307407

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  28. pcDNA3-PKAsub(T/A)-RLuc8N-NES

    Plasmid
    #138212
    Purpose
    Encodes negative-control substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub(T/A)-RLuc8N-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138212-plasmid-map-sequence-id-307414

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  29. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Article
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86708-plasmid-map-sequence-id-317051

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Showing: 3601 - 3630 of 3796 results