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pAS2-1-Mrt4

Plasmid

#99997

Purpose

Express yeast Mrt4 with Y2H binding domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Mrt4 (MRT4 Budding Yeast)

Tags

Y2H binding domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pACT2-Mrt4

Plasmid

#99998

Purpose

Express yeast Mrt4 with Y2H activation domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Mrt4 (MRT4 Budding Yeast)

Tags

Y2H activation domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pACT2-Esf1

Plasmid

#99988

Purpose

Express yeast Esf1 with Y2H activation domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Esf1 (ESF1 Budding Yeast)

Tags

Y2H activation domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pACT2-Utp15

Plasmid

#99978

Purpose

Express yeast Utp15 with Y2H activation domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Utp15 (UTP15 Budding Yeast)

Tags

Y2H activation domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAS2-1-Utp15

Plasmid

#99977

Purpose

Express yeast Utp15 with Y2H binding domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Utp15 (UTP15 Budding Yeast)

Tags

Y2H binding domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only
pDONR221-Cka2

Plasmid

#99980

Purpose

Shuttle yeast Cka2 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Cka2 (CKA2 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Utp16

Plasmid

#99985

Purpose

Shuttle yeast Utp16 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Utp16 (BUD21 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM30C

Plasmid

#87083

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM30C (Trim30c Mouse)

Expression

Mammalian

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pLPC-Myc-TRF2ZNF9-11

Plasmid

#87180

Purpose

Retroviral stable expression of chimeric protein TRF2ZNF9-11

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

chimeric protein TRF2ZNF9-11

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLPC-Myc-TRF2ZNF1-8

Plasmid

#87179

Purpose

Retroviral stable expression of chimeric protein TRF2ZNF1-8

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

chimeric protein TRF2ZNF1-8

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX2T E7 del PTLHE

Plasmid

#13637

Depositor

Karl Munger

Article

Wu et al J Virol. 1993 Apr . 67(4):2402-7.

Insert

HPV 16 E7 del PTLHE (E7 Human)

Tags

GST

Expression

Bacterial

Mutation

del aa 6-10

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pLTR-RD114A

Plasmid

#17576

Depositor

Jakob Reiser

Article

Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

Insert

RD114 envelope glycoprotein

Use

Lentiviral

Expression

Mammalian

Available Since

April 14, 2008

Availability

Industry, Academic Institutions, and Nonprofits

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pU6-tevopreq1-GG-acceptor

Plasmid

#174038

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCHAC-mt-mKeima

Plasmid

#72342

Purpose

retrovirus construct for stable expressing mt-mKeima

Depositor

Richard Youle

Article

Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

Insert

mt-mKeima

Use

Retroviral

Available Since

Jan. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223 NRF2

Plasmid

#118708

Purpose

entry clone for NRF2

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

NRF2

Expression

Mammalian

Mutation

wt

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLII12Pglu-700uDelta6

Plasmid

#17866

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLII12Pglu-700uDelta6

Tags

Citrine

Expression

Mammalian

Mutation

700uDelta6, ECFP insert in mglB

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-p300

Plasmid

#23252

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

p300 (EP300 Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2010

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19

Plasmid

#78597

Purpose

Express flag-USP19 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 (USP19 Human)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD-LSSmOrange

Plasmid

#37129

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

His

Expression

Bacterial

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

araB

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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RpHLuorin2

Plasmid

#180227

Purpose

for bacterial expression of recombinant ratiometric pHluorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2

Tags

His-tag followed by thrombin cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-mNAMPT

Plasmid

#187887

Purpose

Gal4/UAS expression of mammalian NAMPT

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

mNAMPT

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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2X_pX458_pSpCas9(BB)-2A-GFP

Plasmid

#172221

Purpose

Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Type

Empty backbone

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

Cbh

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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mScarlet-EEA1

Plasmid

#169067

Purpose

Express human EEA1 in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

EEA1 (EEA1 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EF06R

Plasmid

#42940

Depositor

Eline Luning Prak

Article

Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

Insert

Human L1 element (L1RE1 Human)

Tags

EGFP (opposite strand)

Expression

Mammalian

Promoter

CMV MIE, L1 5'UTR

Available Since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

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pIF1079

Plasmid

#237445

Purpose

Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.

Depositor

IIya Finkelstein

Article

Buffington et al bioRxiv 2024.07.21.604473; doi: 10.1101/

Inserts

SpCas9
Efe1-RT
EMX1 sgRNA
Efe1-ncRNA targeting EMX1

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG, H1, and U6

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCEP-Puro-TetOn-L1RP-ORF1_mNeonGreen2

Plasmid

#202566

Purpose

Expresses an endogenous-like L1RP LINE-1 element with a C-terminal mNeonGreen2 fluorophore on the ORF1 protein for fluorescence microscopy

Depositor

Liam Holt

Article

Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

Insert

LINE-1

Tags

mNeonGreen2 on ORF1p

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pME_G_E_004_UAV_mScarlet-I

Plasmid

#235974

Purpose

Universal acceptor vector with mScarlet-I placeholder for lvl0 assembly

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

UAV_mScar

Use

Synthetic Biology

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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mTurquoise2

Plasmid

#198196

Purpose

Spectral correction: Expression of mTurquoise2

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

mTurquoise2

Expression

Mammalian

Mutation

A206K

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry- SEC61B

Plasmid

#121160

Purpose

Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

SEC61B (SEC61B )

Expression

Mammalian

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pmGFP-Sec16L

Plasmid

#15776

Depositor

Benjamin Glick

Article

Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

Insert

Sec16L (SEC16A Human)

Tags

mGFP

Expression

Mammalian

Mutation

N/A

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

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