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pMSCV-PIG-miR-22

Plasmid

#64229

Purpose

Expresses miR-22 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR22 (MIR22, MIR22HG Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pUPD2+E-Luc

Plasmid

#170873

Purpose

Phytobrick - E-Luc Luciferase CDS

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Mutation

c.375C>T to remove BbsI site

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pRATIO1267

Plasmid

#141301

Purpose

Gateway cloning compatible dual bioluminescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

gLUC and redLUC

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRATIO4214

Plasmid

#141314

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mCerulean and mScarlet-I

Expression

Plant

Promoter

UBQ10 promoter

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRS426-GFP-Osh1-PH

Plasmid

#71372

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Osh1-PH

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa267-388

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pGEX2TK-Skm1-PH

Plasmid

#71371

Purpose

bacterial expression of PH domain for phosphoinositide binding assay

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Skm1-PH

Tags

GST

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa1-132

Promoter

tac

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pRATIO1112

Plasmid

#141066

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mScarlet-I and mVenus

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMDC32-SEIPIN1

Plasmid

#96973

Purpose

Express Arabidopsis SEIPIN1 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Expression

Plant

Promoter

35S

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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p416GPD-PYK1

Plasmid

#34604

Depositor

Markus Ralser

Article

Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

Insert

PYK1

Expression

Yeast

Promoter

GPD

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR SD Age HsSAS-6

Plasmid

#46381

Purpose

For expression of C-terminal fusions.

Depositor

Pierre Gonczy

Article

Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

Insert

SAS-6 (SASS6 Human)

Use

Entry vector

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pRATIO2212

Plasmid

#141302

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mScarlet-I and mVenus

Expression

Plant

Promoter

UBQ10 promoter

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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PH-dL5**

Plasmid

#96946

Purpose

PLC pleckstrin homology domain linked to FAP for CALI validation

Depositor

Marcel Bruchez

Article

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

Pleckstrin homology domain

Tags

dL5 and mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA ZNHIT3

Plasmid

#110953

Purpose

Transient expression of HA ZNHIT3

Depositor

David Sabatini

Article

Wyant et al Science. 2018 May 18;360(6390):751-758.

Insert

ZNHIT3 (ZNHIT3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEN_TmiRc3_shURB5

Plasmid

#81066

Purpose

Gateway entry vector encoding shRNA to UBR5

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

shRNA targeting UBR5

Use

Cloning

Promoter

N/A

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-PH-dL5**

Plasmid

#96947

Purpose

Expresses EGFP-PLC pleckstrin homology domain linked to FAP for CALI validation

Depositor

Marcel Bruchez

Article

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

Pleckstrin homology domain

Tags

EGFP and dL5

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-RA-HRas-IRES2-Lyn-iRFP682

Plasmid

#180584

Purpose

RA-Hras and Lyn-iRFP682

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

RA-Hras and Lyn-iRFP682

Expression

Mammalian

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Sclt1

Plasmid

#128873

Purpose

For mammalian expression of mouse Sclt1

Depositor

Aimin Liu

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Sclt1 (Sclt1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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hygII-UT-ABAleon2.15

Plasmid

#106983

Purpose

ABAleon2.15 in plant expression vector (Hygromycin resistance)

Depositor

Julian Schroeder

Article

Waadt et al Elife. 2014 Apr 15;3:e01739. doi: 10.755

Insert

ABAleon2.1

Expression

Plant

Promoter

UBQ10

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAT206/Sept7shRNA

Plasmid

#38299

Depositor

Matthew Krummel

Article

Tooley et al Nat Cell Biol. 2009 Jan;11(1):17-26. Epu

Insert

Septin 7 (Septin7 Mouse)

Expression

Mammalian

Promoter

Human U6

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only
Rap-MESA-20-M-Gal4

Plasmid

#84554

Purpose

MESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivator

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

Rap-MESA-FRB-20-M-Gal4

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEYFPC1-ECFP/GgVcl 1-419 control 1 FRET

Plasmid

#46277

Depositor

Susan Craig

Article

Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

Insert

ECFP + Vcl 1-419 (VCL Chicken)

Tags

EYFP

Expression

Mammalian

Mutation

aa 1-419

Promoter

CMV

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-30b

Plasmid

#64232

Purpose

Expresses miR-30b in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-30b (MIR30B Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA HA FAK Y861F

Plasmid

#50508

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y861F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pRD119 (PCNA GFP)

Plasmid

#14822

Depositor

Robert Duronio

Article

Thacker et al Curr Biol. 2003 Jan 8. 13(1):53-8.

Insert

PCNA-GFP (PCNA Fly)

Tags

GFP

Expression

Bacterial

Available Since

July 30, 2007

Availability

Academic Institutions and Nonprofits only
CTAG_mScarlet-I

Plasmid

#136118

Purpose

FP for C-term fusion with CDS12

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG-mScarletI(noATG)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCB150-2_GFP-EB1

Plasmid

#46364

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

EB1 (MAPRE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

pBABE

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hRBM34-HA

Plasmid

#165464

Purpose

Human RBM34 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

RNA binding motif protein 34 (RBM34 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TmiRc3_shNT

Plasmid

#81067

Purpose

Gateway entry vector encoding non-targeting shRNA

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

non-targeting shRNA

Use

Cloning

Promoter

N/A

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pH6Z-806

Plasmid

#85255

Purpose

Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

Depositor

Philip Pellett

Article

Lindquester et al Arch Virol. 1996;141(2):367-79.

Insert

fragment of the human herpesvirus 6B strain Z29 genome

Use

Cloning vector

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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piiTRE-Bi-SG-Tii

Plasmid

#26121

Depositor

Liqun Luo

Article

Li et al PLoS One. 2010 . 5(7):e11503.

Insert

Synaptophysin (Syp Mouse)

Tags

mut4EGFP

Expression

Mammalian

Available Since

Nov. 1, 2010

Availability

Academic Institutions and Nonprofits only