We narrowed to 5,634 results for: pcas

1. hPLD3-sgRNA-Cas9_mcherry

   Plasmid

   #199342

   Purpose

   encodes sgRNA for human PLD3 KO, (target Exon 7) plus Cas9-P2A-mCherry

   Depositor

   David Nemazee

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   P2A-RFP

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. hPLD4-sgRNA-Cas9_mcherry

   Plasmid

   #199343

   Purpose

   encodes sgRNA for human PLD4 KO, (target Exon 5) plus Cas9-P2A-mCherry

   Depositor

   David Nemazee

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Mutation

   sgRNA: accagtagtatgaagccacg

   Promoter

   human U6

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti.Cas9.BFP.Blast

   Plasmid

   #196714

   Purpose

   WT SpCas9 with BSD-TagBFP for selection and sorting

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAVS1-CAG-hCAS9-neo

   Plasmid

   #166026

   Purpose

   AAVS1 targeting vector carrying CAG-SpCas9-neo

   Depositor

   Kosuke Yusa

   Insert

   AAVS1-CAGCas9IRESneo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLXIN2-RFP

   Plasmid

   #99205

   Purpose

   RFP expressing bicistronic retroviral vector

   Depositor

   Saroj Mathupala

   Insert

   Red fluorescent protein
   

   Use

   Retroviral; Bicistronic retroviral expression vec…

   Tags

   Expression

   Mammalian

   Mutation

   The dsRed cDNA was PCR amplified off AddGene plas…

   Promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. BII-gR-PnTW

   Plasmid

   #133356

   Purpose

   Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS

   Depositor

   Blair Madison

   Insert

   tdTomato-NLS
   

   Use

   Tags

   HA-tagged tdTomato

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CaSpeR dhd 3X FLAG

   Plasmid

   #113002

   Purpose

   P element vector for transposon insertion of Flag-tagged Drosophila dhd into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Insert

   dhd (dhd Fly)
   

   Use

   P insertion vector

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. mPLD3-sgRNA-Cas9-mcherry

   Plasmid

   #199700

   Purpose

   encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry

   Depositor

   David Nemazee

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   P2A-RFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AtS/MAR10 insulator sequence (GB3459)

   Plasmid

   #160639

   Purpose

   pEGB3a2 AtS/MAR10

   Depositor

   Diego Orzaez

   Insert

   AtS/MAR10 insulator sequence
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. C2 H5 png-Ha/His in Casper

    Plasmid

    #112969

    Purpose

    P element vector for transposon insertion of png-HA/His into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Insert

    png-HA/His (png Fly)
    

    Use

    P insertion vector

    Tags

    HA/His epitope

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHes1-DsRed

    Plasmid

    #13767

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Hes1 promoter
    

    Use

    Tags

    DsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLexm

    Plasmid

    #99844

    Purpose

    mammalian expression

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNSU299_GAL2

    Plasmid

    #166088

    Purpose

    Plasmid for constituive spCas9 and tet-inducible GAL2 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    GAL2 (GAL2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)

    Plasmid

    #160629

    Purpose

    TU for the expression of LbCas12a

    Depositor

    Diego Orzaez

    Insert

    AtUBQ10-:LbCpf1(Cas12a):Tnos
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    UBQ10

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNrl-DsRed

    Plasmid

    #13764

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Nrl promoter
    

    Use

    Tags

    DsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCVA

    Plasmid

    #32470

    Depositor

    Yule Liu

    Article

    Tang et al Plant Physiol. 2010 Jun;153(2):632-41. E

    Type

    Empty backbone

    Use

    Plant expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCVB

    Plasmid

    #32471

    Depositor

    Yule Liu

    Article

    Tang et al Plant Physiol. 2010 Jun;153(2):632-41. E

    Type

    Empty backbone

    Use

    Plant expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNSU299_SEO1

    Plasmid

    #166076

    Purpose

    Plasmid for constituive spCas9 and tet-inducible SEO1 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    SEO1 (SEO1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSBtet-RB-PE2

    Plasmid

    #173903

    Purpose

    SB-transposon with inducible expression of SpCas9 PE2

    Depositor

    Ronald Cohn

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCC1R

    Plasmid

    #213497

    Purpose

    Encodes the receptor array and inducible aiiA gene of the three-node Turing circuit

    Depositor

    Mark Isalan

    Inserts

    pcaU
    nahR
    rpaR (rpaR )
    phlF
    cinR
    vanR
    aiiA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSBbi-RB-PE2

    Plasmid

    #173902

    Purpose

    SB-transposon with constitutive expression of SpCas9 PE2

    Depositor

    Ronald Cohn

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pNSU299_MET16

    Plasmid

    #166091

    Purpose

    Plasmid for constituive spCas9 and tet-inducible MET16 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    MET16 (MET16 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    May 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pNSU299_CPR1_1

    Plasmid

    #166074

    Purpose

    Plasmid for constituive spCas9 and tet-inducible CPR1 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    CPR1 (CPR1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNSU299_RRT8

    Plasmid

    #166080

    Purpose

    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RRT8 for double stranded break formation in yeast.

    Depositor

    Rebecca Burgess

    Insert

    Promoter of RRT8 (RRT8 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNSU299_CPR1_2

    Plasmid

    #166071

    Purpose

    Plasmid for constituive spCas9 expression and tet-inducible expression of an sgRNA targeting an intergenic site near CPR1 for double stranded break formation in yeast.

    Depositor

    Rebecca Burgess

    Insert

    Intergenic region near CPR1
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNSU299_GAL7

    Plasmid

    #166089

    Purpose

    Plasmid for constituive spCas9 and tet-inducible GAL7 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    GAL7 (GAL7 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNSU299_CUP1-1

    Plasmid

    #166086

    Purpose

    Plasmid for constituive spCas9 and tet-inducible CUP1-1 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    CUP1-1 (CUP1-1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pNSU299_GLK1

    Plasmid

    #166090

    Purpose

    Plasmid for constituive spCas9 and tet-inducible GLK1 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    GLK1 (GLK1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNSU299_LHS1

    Plasmid

    #166092

    Purpose

    Plasmid for constituive spCas9 and tet-inducible LHS1 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    LHS1 (LHS1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNSU299_APL3

    Plasmid

    #166073

    Purpose

    Plasmid for constituive spCas9 and tet-inducible APL1-targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    APL3 (APL3 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge