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Showing: 2401 - 2420 of 66151 results

  1. pICE-HA-NLS-I-PpoI

    Plasmid
    #46963
    Purpose
    For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.
    Depositor
    Steve Jackson
    Publication
    Britton et al J Cell Biol. 2013 Jul 29.
    Insert
    NLS-I-PpoI
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV-TET
    Available since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46963-plasmid-map-sequence-id-66854

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  3. pMaster12

    Plasmid
    #58527
    Purpose
    Reprogram somatic cells to iPS cells
    Depositor
    Mario Capecchi
    Publication
    Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    neo
    tk
    oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    Myc (MYC Human)
    EBNA-1
    Amp
    Lin28 (LIN28A Human)
    NANOG (NANOG Human)
    IRES
    NR5A2 (NR5A2 Human)
    miR302/367 (MIR367, MIR302A Human)
    Expression
    Mammalian
    Available since
    Feb. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58527-plasmid-map-sequence-id-138420

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  8. delta PE OCT4-GFP-2A-PURO transgenic reporter

    Plasmid
    #52380
    Purpose
    BAC recombineering generated construct, used as a reporter for OCT4 expression
    Depositor
    Jacob Hanna
    Publication
    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GFP-2A-puro
    OCT4 (POU5F1 Human)
    Expression
    Mammalian
    Mutation
    deleted -1620 - -1011 upstream from OCT4 ATG
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52380-plasmid-map-sequence-id-82023

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  9. pMH-SFB-BRCA2

    Plasmid
    #99395
    Purpose
    Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA2
    Depositor
    Junjie Chen, Michael Huen
    Publication
    Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BRCA2 (BRCA2 Human)
    Tags
    SFB (S protein-FLAG-Streptavidin binding peptide)
    Expression
    Mammalian
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99395-plasmid-map-sequence-id-190616

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  10. pCMV-PE7-P2A-hMLH1dn

    Plasmid
    #222997
    Purpose
    Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)
    Depositor
    Brittany Adamson
    Publication
    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE7-P2A-hMLH1dn
    Tags
    SV40 bpNLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Deletion of residues 754-756 in MLH1 as described…
    Promoter
    CMV
    Available since
    Aug. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    222997-plasmid-map-sequence-id-454423

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  18. Villinpromoter-blue-FlpOERT2

    Plasmid
    #67278
    Purpose
    4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Depositor
    Jannik Elverløv-Jakobsen
    Publication
    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do
    Insert
    Villin-mTQ2-P2A-FlpO-ERT2
    Tags
    mTQ2, linked to FlpO through an P2A ribosomal ski…
    Expression
    Mammalian
    Promoter
    Villin
    Available since
    July 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    67278-plasmid-map-sequence-id-115887

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  19. Str-KDEL_SBP-EGFP-GPI

    Plasmid
    #65294
    Purpose
    synchronize trafficking of EGFP-GPI from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPI anchor
    Tags
    EGFP and IL-2 Signal Sequence and Streptavidin Bi…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65294-plasmid-map-sequence-id-110502

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Showing: 2401 - 2420 of 66151 results