We narrowed to 13,107 results for: CRISPR-Cas9

1. Lenti-eCas9-sgMIB1

   Plasmid

   #140239

   Purpose

   Lentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   sgRNA targeting human MIB1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-spCas9-N57

   Plasmid

   #169920

   Purpose

   Expression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.

   Depositor

   Zhili Rong

   Article

   Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

   Insert

   SpCas9-N57
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   N57 is the PAI domain of SB100X

   Promoter

   CAG

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. xCas9(3.6)-BE4

   Plasmid

   #108386

   Purpose

   Mammalian expression vector for xCas9(3.6)-BE4

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9(3.6)-BE4
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. xCas9(3.6)-BE3

   Plasmid

   #108385

   Purpose

   Mammalian expression vector for xCas9(3.6)-BE3

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9(3.6)-BE3
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ150-Cas9(dpiRNA)

   Plasmid

   #107940

   Purpose

   eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.

   Depositor

   Heng-Chi Lee

   Article

   Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

   Insert

   Cas9(dpiRNA)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   eft-3

   Available since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-Sa-SauriCas9

   Plasmid

   #135967

   Purpose

   Expresses Sa-SauriCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRGEB-VirD2-Cas9

   Plasmid

   #139803

   Purpose

   Expressing VirD-Cas9 fusion under ubiquitin promoter

   Depositor

   Magdy Mahfouz

   Article

   Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

   Insert

   VirD2-Cas9
   

   Use

   Tags

   VirD2-Cas9

   Expression

   Plant

   Mutation

   Promoter

   Ubiquitin

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX459-HypaCas9-TUBA1B_sgRNA

   Plasmid

   #183889

   Purpose

   pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   TUBA1B sgRNA spacer (TUBA1B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-TAC-OKMS-Cas9

   Plasmid

   #120353

   Purpose

   piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OKMS cassette
   

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Klf4 [10-483]

   Promoter

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. eSpCas9(1.1)_No_FLAG_ATP1A1-G7

    Plasmid

    #173203

    Purpose

    Expresses the ATP1A1 G7 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 17. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G7 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-AGG-SpCas9

    Plasmid

    #214603

    Purpose

    encode SpCas9; in vitro transcribed modified RNA vector

    Depositor

    Yuxuan Guo

    Article

    Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

    Insert

    SpCas9
    

    Use

    CRISPR and Unspecified

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. L2_NOP1gRNA-Cas9-CsA

    Plasmid

    #136139

    Purpose

    Plasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:HygR p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

    Plasmid

    #187960

    Purpose

    AID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

    Plasmid

    #187963

    Purpose

    mAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Inserts

    AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
    osTIR1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid

    #218710

    Purpose

    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo

    Plasmid

    #120555

    Purpose

    Express Cas9, 2xNES, 2 tandem ERT2 and GCN4 in mammalian cells

    Depositor

    Yu Wang

    Article

    Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

    Insert

    Cas9, NES, ERT2 and GCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)

    Plasmid

    #47869

    Purpose

    All-in-one plasmid targeting tdTomato

    Depositor

    Erik Sontheimer, James Thomson

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Inserts

    NmCas9
    U6pr-tracrRNA
    tdTomato crRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP

    Plasmid

    #127560

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Cas9-T2A-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    EGFP-D102G see depositor comments below

    Promoter

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFUGW hUbC-Sga-dCas9-KRAB-T2A-GFP

    Plasmid

    #227009

    Purpose

    Lentiviral Expression of dCas9-KRAB from S. gallolyticus fused to the repressive KRAB domain

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFUGW hUbC-Sin-dCas9-KRAB-T2A-GFP

    Plasmid

    #227010

    Purpose

    Lentiviral Expression of dCas9-KRAB from S. iniae fused to the repressive KRAB domain

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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